TM29基因在番茄花序及果实发育中作用

Down-RegulationofTM29,aTomatoSEPALLATA

Homolog,CausesParthenocarpicFruitDevelopmentand

FloralReversionCharlesAmpomah-Dwamena,BretA.Morris2,PaulSutherland,BruceVeit3,andJia-LongYaoZhangYunyi09/06/16Down-RegulationofTM29,aTom1SEPfunctionsinArabidopsisSequenceanalysisofTM29SpatialandtemporalexpressionofTM29intomatoTM29transgenicplantphenotypesTM29proteininteractswithSlHDACsConclusionReferencesContextSEPfunctionsinArabidopsisCo2SEPfunctionsinArabidopsisBandCfloralorganidentityfunctionsrequire

SEPALLATA

MADS-boxgenesPelazetal.,Nature

405,200-203(11May2000)SEPfunctionsinArabidopsisB3SEPfunctionsinArabidopsisSequenceanalysisofTM29SpatialandtemporalexpressionofTM29intomatoTM29transgenicplantphenotypesTM29proteininteractswithSlHDACsConclusionReferencesContextSEPfunctionsinArabidopsisCo4TM29GroupstotheSEPSubfamilyPhylogeneticanalysisSequenceanalysisTM29GroupstotheSEPSubfami5SEPfunctionsinArabidopsisSequenceanalysisofTM29SpatialandtemporalexpressionofTM29intomatoTM29transgenicplantphenotypesTM29proteininteractswithSlHDACsConclusionReferencesContextSEPfunctionsinArabidopsisCo6SpatialandTemporalExpressionofTM29inTomatofb,Flowerbudsfr,Youngfruitslf,Youngleavessh,Shoottipsrt,

RootsNorthern-blothybridizationpa,Pre-anthesisa,AnthesisDPA,Fruitat3,6,14,and21dayspost-anthesislf,

YoungleavesTM29expressionoccursfromtheearlystagesofflowerdevelopmenttotheearlystagesoffruitdevelopment.SpatialandTemporalExpressio7SpatialandTemporalExpressionofTM29inTomatoSympodialbudFloral(fm)/Inflorescence(im)meristemSepalOvaryInsituhybridizationsb,sympodialbud;lf,leaf;fm,floralmeristem;im,inflorescencemeristem;vb,vascularbundle;se,sepalprimordiuman,antherregion;ov,ovaryprimordium;per,pericarpregion;st,stamen;tap,tapetalregion;SpatialandTemporalExpressio8SEPfunctionsinArabidopsisSequenceanalysisofTM29SpatialandtemporalexpressionofTM29intomatoTM29transgenicplantphenotypesTM29proteininteractswithSlHDACsConclusionReferencesContextSEPfunctionsinArabidopsisCo9TM29TransgenicPlants

PhenotypesSepallata-likeflowersDelayedsenescencepetalstamenTM29TransgenicPlants

Phenoty10TM29TransgenicPlants

PhenotypesDelayedripeningprocessEctopicshootsgrewinsidethefuritTM29TransgenicPlants

Phenoty11TM29TransgenicPlants

Phenotypesel,Ectopicleaf;ef,Ectopicflower;fr,Fruit;es,Ectopicinflorescence;ov,ovule;per,Pericarp;pl,Placenta;sd,SeedTM29mayberequiredforthemaintenanceoffloralmeristemidentifyTM29TransgenicPlants

Phenoty12SEPfunctionsinArabidopsisSequenceanalysisofTM29SpatialandtemporalexpressionofTM29intomatoTM29transgenicplantphenotypesTM29proteininteractswithSlHDACsConclusionReferencesContextSEPfunctionsinArabidopsisCo13vTM29proteininteractswithSlHDACs

SlHDACs=SolanumlycopersicumHistoneDeacetylasesYeasttwo-hybridassayPull-downassayvTM29proteininteractswithS14SEPfunctionsinArabidopsisSequenceanalysisofTM29SpatialandtemporalexpressionofTM29intomatoTM29transgenicplantphenotypesTM29protein

interactswithSlHDACsConclusionReferencesContextSEPfunctionsinArabidopsisCo15TM29sharesahighaminoacidsequencehomologytotheArabidopsis

SEP1,2,and3genesTM29isinvolvedinfloralorgansdevelopmentlikeSEPgenesTM29mayalsoplayaroleinthemaintenanceoffloralmeristemidentityandfruitdevelopmentintomatoTM29proteinmayrecruitHDACstoregulategeneexpressioninreproductivedevelopmentintomatoConclusionTM29sharesahighaminoacid16SEPfunctionsinArabidopsisSequenceanalysisofTM29SpatialandtemporalexpressionofTM29intomatoTM29transgenicplantphenotypesTM29proteininteractswithSlHDACsConclusionReferencesContextSEPfunctionsinArabidopsisCo17SorayaPelaz1,GaryS.Ditta1,ElviraBaumann2,EllenWisman2&MartinF.Yanofsky1(2000)BandCfloralorganidentityfunctionsrequireSEPALLATAMADS-boxgenes.Nature405,200-203CharlesAmpomah-Dwamena,BretA.Morris,2PaulSutherland,BruceVeit,3andJia-LongYao4,*(2002)Down-RegulationofTM29,aTomatoSEPALLATAHomolog,CausesParthenocarpicFruitDevelopmentandFloralReversion.PlantPhysiol.2002Oct;130(2):605–617FlanaganCA1,MaH(1994)SpatiallyandtemporallyregulatedexpressionoftheMADS-boxgeneAGL2inwild-typeandmutantarabidopsisflowers.PlantMolBiol.1994Oct;26(2):581-95Linma