类器官研究中的细胞因子

类器官研究中的细胞因子选择与应用李田子,Ph.D.

高级产品经理北京义翘神州科技股份有限公司09-2024义翘神州CONTENT类器官培养的关键细胞因子“配方”1经类器官培养验证的细胞因子2义翘神州类器官培养的关键细胞因子“配方”01义翘神州类器官研究-市场情况义翘神州√√√√√Homan./10.1002/adbi.202200334HanbyeolLee;etal./10.51335/organoid.2021.1.e11.Source:终端客户可能应用Biotchenology&Pharmaceuticalindustries主要用于建立类器官模型和类器官芯片以及基于模型的药物开发，药敏反应等Academic&ResearchinstitutesHospitals&DiagnosticCenters类器官研究-研究进展和潜在应用义翘神州Source:NCBI-PubMedCentralhttps://ClinicalTBasicresearchBiobankingPrecisionmedicine-Responsetodrugs-ResourcesforregenerativemedicinecoupledwithgeneticengineeringDiseasemodeling-Mechanismsofhumandiseases-InheritablegeneticdisordersDrug-screeninganalysesJihoonKim;etal./10.1038/s41580-020-0259-3.类器官研究-创新模型2024年8月14日,Nature刊发了一篇关于具有自体组织驻留免疫系统的肠道类器官（IIOs）模型传统的肠道类器官主要关注上皮细胞的分化和功能，而未将免疫系统纳入其中炎症性肠病（IBD）和癌症等疾病中，免疫反应会影响疾病进展和治疗效果文献中建立的类器官可能能更准确地表现出肠道病生理的状态义翘神州TimothyRecaldinetal./10.1038/s41586-024-07791-5.SchematicoverviewofestablishmentofautologousIIOs.肠道类器官-药物筛选应用场景疾病研究题目临床#药物筛选结直肠癌ProspectiveObservationontheAccuracyofinVitroScreeningofColorectalCancerChemotherapyDrugsBasedonOrganoids-ona-chipNCT04996355TheCultureofAdvanced/Recurrent/MetastaticColorectalCancerOrganoidsandDrugScreeningNCT05304741Patient-derivedOrganoidsofRAS/RAFWild-typeMetastaticRightColonCancertoTesttheSensitivityandClinicalConsistencyofCombinedTreatmentofCetuximab.NCT04906733AProspectiveMulticenterRandomizedControlledTrialoftheClinicalEfficacyofNeoadjuvantTherapyBasedonOrganoidsDrugSensitivityVersusEmpiricalNeoadjuvantTherapyintheTreatmentofAdvancedRectalCancerNCT05352165义翘神州QinyingWang;etal./10.1038/s41392-022-01194-6.RSPO-1EGFNogginWnt肠道类器官激活小肠隐窝的Wnt信号协助小肠增殖隐窝数量增多维持肠道干细胞义翘神州ToshiroSato;etal./10.1053/j.gastro.2011.07.050.肠道类器官培养-主要细胞因子Figure.Human-derivedorganoids类器官研究-相关细胞因子BrainBreastLungProstatePancreaticHeartIntestinalGastricLiverEGFEGFEGFEGFEGFEGFFGF2FGF2FGF2FGF2FGF2FGF2FGF2FGF7TGFB1FGF10FGF10FGF10FGF10FGF10FGF10FGF10NOGNOGNOGNOGNOGNOGNOGNOGINHBAINHBAINHBAINHBAHGFRSPO1RSPO1RSPO1RSPO1RSPO1RSPO1RSPO1OSMDKK1DKK1GDNFLIF义翘神州OVARYBRAINBREASTLUNGSKIDNEYBLADDERPROSTATEEYESINNEREARESOPHAGUSSTOMACHINTESTINEandCOLONLIVERPANCREASBenedettaArtegiani;etal./10.1093/hmg/ddy187.经类器官培养验证的细胞因子02义翘神州细胞因子产品线特点概述GMP认证书250+细胞因子类分子，800+细胞因子类产品按照ISO9001:2015和ISO13485:2016质量管理体系要求进行生产涉及免疫细胞培养、干细胞研究、类器官培养等热门应用领域覆盖生长因子、趋化因子、干扰素、白介素、淋巴因子等严格质控造就更优细胞活性高批间一致性高纯度（20+GMP级细胞因子）义翘神州2024年值得关注的生长因子供应商细胞因子产品设计种属多：Human、Mouse、Monkey等，尽可能满足不同的动物实验模型倾向于无标签或者小标签真核或者原核表达系统低内毒素活性有竞争力应用场景数据展示义翘神州多种属：Human、Mouse、Monkey等，尽可能满足不同动物实验模型的需求义翘神州小鼠膀胱类器官培养MouseBladderOrganoidsafter14daysinculture25ng/mLofRecombinantMouseKGF/FGF-7Protein(50394-M07E)wasusedfor2weeksinBMEsubmergedinmousebladdermedia.DataprovidedbyDr.YinatWashingtonUniversity,St.Louis,MO.经过14天的培养：细胞因子产品设计LPS处理的胚状体中胚层的生物标志物会消失且不能分化成有功能的成骨细胞LPS处理的胚状体会出现凋亡的现象义翘神州KavithaSivasubramaniyan;etal./10.2217/1746073.细胞因子产品设计低内毒素Panel(A)showsnormal5-day-oldembryoidbodiesand(B)shows5-day-oldembryoidbodiesexposedtolipopolysaccharidesfor48h.Picturewereacquiredat10×magnifications.Cometassayshowingthedegreeofapoptosisinducedincontrolandlipopolysaccharides-treatedembryoidbodies.CD14+

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DCs义翘神州细胞因子产品设计应用场景数据展示-1：免疫细胞应用场景义翘神州产品详情页下拉：点开产品详情页：细胞因子产品设计应用场景数据展示-2：类器官应用场景iPSC-derivedhumanvascularorganoids(Day7)wereculturedwithFGF2(10014-HNAE),VEGF165(11066-HNAH),EGF(10605-HNAE).Redarrowsrepresentvascularorganoids.Imagetakenat10xmagnification.DataprovidedbyDr.WangatSchoolofBasicMedicalSciencePekingUniversity义翘神州活性比较数据批间一致性数据MeasuredinacellproliferationassayusingBalb/C3T3mouseembryonicfibroblasts.EGF

(Cat#:10605-HNAE)义翘神州批次1义翘神州批次2义翘神州批次3国外知名品牌义翘神州批次1义翘神州批次2高纯度（≥95%）FGF2(Cat#:10014-HNAE)VEGF165(Cat#:11066-HNAH)血管类器官培养类器官培养相关产品数据高纯度（≥95%）类器官相关产品的引用文献义翘神州文献名称(年份)-类器官类型影响因子所用货号HumanearlysyncytiotrophoblastsarehighlysusceptibletoSARS-CoV-2infection(2022)-滋养层类器官11.710463-HNAS(HGF);11083-HNAS(RSPO1)Single-celltranscriptomicsrevealscellatlasandidentifiescyclingtumorcellsresponsibleforrecurrenceinameloblastoma(2024)-成釉细胞瘤类器官10.810014-HNAE(FGF2)CUL4B-DDB1-COP1-mediatedUTXdownregulationpromotescolorectalcancerprogression(2023)-结直肠癌类器官9.411083-HNAS(RSPO1);50688-M02H(NOG);50482-MNCH(EGF);10573-HNAE(FGF10)FeedbackactivationofEGFR/wild-typeRASsignalingaxislimitsKRASG12DinhibitorefficacyinKRASG12D-mutatedcolorectalcancer(2023)-结直肠癌类器官6.911083-HNAS(RSPO1);50688-M02H(NOG);50482-MNCH(EGF)EvaluationofB7-H3TargetedImmunotherapyina3DOrganoidModelofCraniopharyngioma(2022)-颅咽管瘤类器官4.810605-HNAE(EGF);10573-HNAE(FGF10)2022~2024类器官相关引用文献数量滋养层类器官培养-文献中产品应用案例扩增潜力干细胞-滋养层干细胞来源的滋养层类器官义翘神州50ng/mLHumanHGF(SinoBiological,10463-HNAS)50ng/mLHumanEGF80ng/mLHumanR-spondin1(SinoBiological,11083-HNAS)培养中用到的细胞因子100ng/mLHumanFGF2DegongRuan;etal./10.1016/j.xcrm.2022.100849.滋养层类器官类器官培养相关产品数据义翘神州活性比较数据批间一致性数据MeasuredbyitsabilitytoinduceactivationofßcateninresponseinaTopflashLuciferaseassayusingHEK293Thumanembryonickidneycells.RSPO1(Cat#:11083-HNAS)国外知名品牌义翘神州义翘神州批次1义翘神州批次2义翘神州批次3活性比较数据批间一致性数据MeasuredbyitsabilitytoneutralizeTGF-betamediatedinhibitiononMv-1-Lucellproliferation.HGF(Cat#:10463-HNAS)义翘神州国外知名品牌义翘神州批次1义翘神州批次2义翘神州批次3肠道类器官培养-文献中产品应用案例1义翘神州AdvancedDMEM/F12medium50ng/mLMouseEGF(SinoBiological,50482-MNCH)100ng/mLMouseNoggin(SinoBiological,50688-M02H)50mg/mLHumanR-spondin1(SinoBiological,11083-HNAS)培养主要组成结直肠癌组织分离培养包埋在Matrigel中接种到12孔板上添加类器官培养基每2~3天更换新鲜培养基结直肠癌类器官用于：药敏试验药物作用机制研究Fenget/10.1038/s41388-023-02676-9.结直肠癌类器官--结直肠癌患者治疗效果的研究肠道类器官培养-文献中产品应用案例2义翘神州AdvancedDMEM/F12medium50ng/mLMouseEGF(SinoBiological,50482-MNCH)100ng/mLMouseNoggin(SinoBiological,50688-M02H)500ng/mLHumanR-spondin1(SinoBiological,11083-HNAS)培养主要组成结直肠癌组织分离培养包埋在Matrigel中接种到48孔板上添加类包含药物的培养基每3天更换新鲜培养基结直肠癌类器官用于：疾病研究药物作用机制研究DakuiLuoet/10.1186/s40164-023-00440-z.100ng/mLHumanFGF10(SinoBiological,10573-HNAE)结直肠癌类器官--疾病研究不同时间点颅咽管瘤类器官的形态

脑类器官培养-文献中产品应用案例义翘神州10ng/mLHumanFGF10(SinoBiological,10573-HNAE)10ng/mLHumanEGF(SinoBiological,10605-HNAE)培养中用到的细胞因子Xiao-YanTang;etal./10.1038/s41392-022-01024-9.脑肿瘤类器官类器官培养相关产品数据义翘神州CellproliferationassayusingBaF3mousepro-BcellstransfectedwithhumanFGFR2b.FGF10(Cat#:10573-HNAE)活性比较数据国外知名品牌义翘神州高纯度（≥95%）CellproliferationassayusingBaF3mousepro-BcellstransfectedwithhumanFGFR2b.FGF7/KGF(Cat#:10210-H07E)活性比较数据国外知名品牌义翘神州高纯度（≥95%）类器官培养相关产品数据义翘神州

InhibitproliferationofMPC-11cells.>1000U/mg活性比较数据义翘神州国外知名品牌

GMP级HumanActivinA

(Cat#:GMP-10429-HNAH)高纯度（≥95%）CellproliferationassayusingTF-1humanerythroleukemiccells.>30,000Units/μg批间一致性数据义翘神州批次1义翘神州批次2

GMP级HumanOSM(Cat#:GMP-10452-HNAH)高纯度（≥95%）经不同类器官培养验证的细胞因子义翘神州人肠癌类器官人结直肠类器官HumanintestinalcancerorganoidswereculturedwithRSPO1(11083-HNAS),EGF(50482-MNCH),NOG(50688-M02H).(Routinelytested).DataprovidedbyD1MedicalTechnologyHumancolorectumorganoidswereculturedwithRSPO1(11083-HNAS),EGF(50482-MNCH),NOG(50688-M02H).(Routinelytested).DataprovidedbyD1MedicalTechnology.义翘神州人肺癌类器官人肺类器官HumanlungcancerorganoidswereculturedwithFGF2(10014-HNAE),FGF4(16043-HNAE),FGF7(10210-H07E),EGF(50482-MNCH),FGF10(10573-HNAE),NOG(50688-M02H),RSPO1(11083-HNAS).(Routinelytested).DataprovidedbyD1MedicalTechnology.HumanlungorganoidswereculturedwithFGF2(10014-HNAE),FGF4(16043-HNAE),FGF7(10210-H07E),EGF(50482-MNCH),FGF10(10573-HNAE),NOG(50688-M02H),RSPO1(11083-HNAS).(Routinelytested).DataprovidedbyD1MedicalTechnology.经不同类器官培养验证的细胞因子义翘神州人肝癌类器官人胆管癌类器官HumanlivercancerorganoidswereculturedwithFGF2(10014-HNAE),HGF(10463-HNAS),FGF7(10210-H07E),EGF(50482-MNCH),FGF10(10573-HNAE),TGFB1(10804-HNAC),NOG(50688-M02H),RSPO1(11083-HNAS).(Routinelytested).DataprovidedbyD1MedicalTechnology.HumancholangiocarcinomasorganoidswereculturedwithFGF2(10014-HNAE),HGF(10463-HNAS),FGF7(10210-H07E),EGF(50482-MNCH),FGF10(10573-HNAE),NOG(50688-M02H),RSPO1(11083-HNAS).(Routinelytested).DataprovidedbyD1MedicalTechnology.经不同类器官培养验证的细胞因子义翘神州人肾癌类器官人肾类器官HumankidneycancerorganoidswereculturedwithFGF2(10014-HNAE),FGF7(10210-H07E),EGF(50482-MNCH),FGF10(10573-HNAE),NOG(50688-M02H),RSPO1(11083-HNAS).(Routinelytested).DataprovidedbyD1MedicalTechnology.HumankidneyorganoidswereculturedwithFGF7(10210-H07E),EGF(50482-MNCH),FGF10(10573-HNAE),NOG(50688-M02H),RSPO1(11083-HNAS),HGF(10463-HNAS),FGF4(16043-HNAE).(Routinelytested).DataprovidedbyD1MedicalTechnology.经不同类器官培养验证的细胞因子义翘神州人胃癌类器官人胃类器官HumangastriccancerorganoidswereculturedwithEGF(50482-MNCH),

FGF10(10573-HNAE),NOG(50688-M02H),RSPO1(11083-HNAS).(Routinelytested).DataprovidedbyD1MedicalTechnologyHumanstomachorganoidsorganoidswereculturedwithEGF(50482-MNCH),FGF10(10573-HNAE),NOG(50688-M02H),RSPO1(11083-HNAS).(Routinelytested).DataprovidedbyD1MedicalTechnology.经不同类器官培养验证的细胞因子义翘神州人卵巢癌类器官人小肠类器官HumansmallintestinalorganoidswereculturedwithIL22(13059-HNAE),FGF10(10573-HNAE),EGF(50482-MNCH),NOG(50688-M02H).(Routinelytested).DataprovidedbyD1MedicalTechnology.Humanovarianorg