DiI-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEDiI(solution)Cat.No.:HY-DY1012CASNo.:41085-99-8分⼦式:C₅₉H₉₇ClN₂O₄分⼦量:933.89作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:-20°C,protectfromlight.Avoidrepeatedfreezingandthawing*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light.Avoidrepeatedfreezingandthawing)BIOLOGICALACTIVITY⽣物活性DiI(solution)⼀种长链碳菁染料。碳菁染料⼴泛⽤作亲脂性⽰踪剂，⽤于标记细胞、细胞器、脂质体、病毒和脂蛋⽩。溶剂及浓度：DMSO：5mM体外研究GeneralProtocol1.PreparingStainSolutionsofDiIa.Prepareworkingsolutions:Dilutethestocksolutionsintoasuitablebuffersuchasserum-freeculturemedium,HBSSorPBStomake1to5μMworkingsolutions.Wedonotrecommendstoringtheaqueoussolutionformorethanoneday.2.Suspensioncellsa.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Thecelldensityis1×106/mL.b.Add1mLofDiIworkingsolution,andthenincubateatroomtemperaturefor5-30minutes.c.Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.d.WashtwicewithPBS,5minuteseachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyorflowcytometry.3.Adherentcellsa.Cultureadherentcellsonsterilecoverslips.b.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor5-30minutes.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEd.Washtwicewithmedium,5minuteseachtime.Observationbyfluorescencemicroscopyorflowcytometry.体内研究DiI-labeledmotoneuronshaveremainedviableforupto4weeksincultureanduptooneyearinvivo[1]REFERENCES[1].DPKuffler,etal.Long-termsurvivalandsproutinginculturebymotoneuronsisolatedfromthespinalcordofadultfrogs.JCompNeurol.1990Dec22;302(4):729-38.[2].GanWB,etal.Multicolor"DiOlistic"labelingofthenervoussystemusinglipophilicdyecombinations.Neuron.2000Aug;27(2):219-25.[3].DPKuffler,etal.Long-termsurvivalandsproutinginculturebymotoneuronsisolatedfromthespinalcordofadultfrogs.JCompNeurol.1990Dec22;302(4):729-38.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchu