LysoTracker-Red-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemELysoTrackerRed(solution)Cat.No.:HY-DY1040CASNo.:231946-72-8分⼦式:C₂₀H₂₄BF₂N₅O分⼦量:399.25作⽤靶点:FluorescentDye;BiochemicalAssayReagents作⽤通路:Others储存⽅式:-20°C,protectfromlight.Avoidrepeatedfreezingandthawing*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light.Avoidrepeatedfreezingandthawing)BIOLOGICALACTIVITY⽣物活性LysoTrackerRed(solution)为红⾊荧光标记的溶酶体探针，具有577/590nm的最⼤激发/发射波长。结构上由⼀个荧光团和相连的弱碱构成，可⾃由穿过细胞膜，⼀般聚集在球形细胞器上，适⽤于观察溶酶体内部⽣物合成及相关发病机理。溶剂及浓度：DMSO：1mM体外研究PreparationofLysoTrackerRedworkingsolution1.Workingfluidconfiguration:1.1Restorethesolutiontoroomtemperatureandconcentrateitatthebottomofthetubebyinstantaneouscentrifugation.1.2Dilute1mMstoragesolutiontotheworkingsolutionusingamediumorappropriatebuffer(suchasPBS).Therecommendedworkingsolutionis50-100nM;Note:PleaseadjusttheconcentrationoftheLysoTrackerRedworkingsolutionaccordingtotheactualsituation,anduseitnow.2.Cellstaining2.1Forsuspensioncells:Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Foradherentcells:Discardthecellculturemedium,andaddtrypsintodissociatecellstomakeasingle-cellsuspension.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.2.2Add1mLofLysoTrackerRedworkingsolution,andthenincubateatroomtemperaturefor5-30minutes.2.3Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.2.4WashtwicewithPBS,5minuteseachtime.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.5Resuspendcellswithserum-freecellculturemediumorPBS,andthendetectbyfluorescencemicroscopeorflowcytometer.REFERENCES[1].PriceOT,etal.Quantitativefluorescenceof5-FU-treatedfetalratlimbsusingconfocallaserscanningmicroscopyandLysotrackerRed.CytometryA.2003;53(1):9-21.[2].PereiraLC,etal.AnautophagicprocessisactivatedinHepG2cellstomediateBDE-100-inducedtoxicity.Toxicology.2017;376:59-65.[3].NagahamaM,etal.CellularvacuolationinducedbyClostridiumperfringensepsilon-toxin.FEBSJ.2011;278(18):3395-3407.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearch