N-Methylmesoporphyrin-IX-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEN-MethylmesoporphyrinIX(solution)Cat.No.:HY-DY1075CASNo.:142234-85-3分⼦式:C₇₀H₈₀N₈O₈分⼦量:1161.46作⽤靶点:G-quadruplex作⽤通路:CellCycle/DNADamage储存⽅式:-20°C,protectfromlight.Avoidrepeatedfreezingandthawing*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light.Avoidrepeatedfreezingandthawing)BIOLOGICALACTIVITY⽣物活性N-MethylmesoporphyrinIX(NMM)(solution)⼀种⼴泛使⽤的G-quadruplexDNA特异性荧光结合剂，是监测Aβ纤颤的有效探针。N-MethylmesoporphyrinIX⼀种原位抑制剂，体外和细胞内Aβ淀粉样蛋⽩⽣成的⾮原位监测器。N-MethylmesoporphyrinIX对G-四链体DNA敏感，但对双链体、三链体和单链形式的DNA没有反应。N-MethylmesoporphyrinIX单独或在单体Aβ环境中不发出荧光，但通过与Aβ组装体堆叠发出强荧光。溶剂及浓度：DMSO：2mM体外研究ByuseofN-MethylmesoporphyrinIX(NMM)asanexsituprobe,NMMisaddedintotheincubatedAβ40solution.TheconcentrationofNMMisfixedat1μM.ToexaminetheinfluenceofRhodamineB,1μMNMMwith0.1μMor0.5μMRhodamineBalongwith10μMAβ40aremeasured.InthestudyofusingNMMasaninsituinhibitor,NMM(10μM)isco-incubatedwithAβ40(50μM)for7daysat37°C.AdditionalNMMisaddedbeforePLmeasurementtomaketheconcentrationofNMMconstant(1μM).ThefluorescencespectraofNMMarecollectedfrom550to700nmwithanexcitationwavelengthof399nm[1].N-MethylmesoporphyrinIX(NMM)issensitivetoG-quadruplexesDNAbuthasnoresponsetoduplexes,triplexesandsingle-strandedformsDNA.UponbindingtoquadruplexDNA,forefficientπ-πstacking,NMMcanadjustitsmacrocyclegeometrytomatchtheterminalfaceofaG-quadruplex,leadingtoanenhancementinitsfluorescence[1].REFERENCES[1].MengLi,etal.N-MethylMesoporphyrinIXasanEffectiveProbeforMonitoringAlzheimer'sDiseaseβ-AmyloidAggregationinLiving1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECells.ACSChemNeurosci.2017Jun21;8(6):1299-1304.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-82