Texas-Red-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemETexasRed(solution)Cat.No.:HY-DY1056CASNo.:60311-02-6分⼦式:C₃₁H₃₀N₂O₇S₂分⼦量:606.71作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性TexasRed(Sulforhodamine101)(solution)⼀种两性罗丹明红⾊荧光染料(excitation/emission:586/605nm)。TexasRed被⼴泛⽤于研究神经元形态，并作为形胶质细胞的细胞类型选择性荧光标记物，在体内和切⽚制备中都有应⽤。溶剂及浓度：DMSO：10mM体外研究Description:TexasRed(Sulforhodamine101)canbeusedasanastrocytemarkerforhippocampalslicesstainingwithredfluorescence[3].Method:Forcellstaining.1.StainhippocampalsliceswithTexasRed(5μM;35min;34°C)incarbogenatedArtificialCerebroSpinalFluid(ACSF).2.Returnhippocampalslicestodye-freeACSFforatleast30minbeforeimaging.3.Useaconfocallaserscanningmicroscopy(ZeissLSM880)forimage.体内研究Invivo,epilepticactivitycanbeinducedbyintra-hippocampalinjectionofsmallvolumesof10μMTexasRedortopicalapplicationof100μM[1].REFERENCES[1].AxelNimmerjahn,etal.Sulforhodamine101asaspecificmarkerofastrogliaintheneocortexinvivo.NatMethods.2004Oct;1(1):31-7.[2].JKang,etal.Sulforhodamine101induceslong-termpotentiationofintrinsicexcitabilityandsynapticefficacyinhippocampalCA1pyramidalneurons.Neuroscience.2010Sep15;169(4):1601-9.[3].WangT,etal.Malfunctionofastrocyteandcholinergicinputisinvolvedinpostoperativeimpairmentofhippocampalsynapticplasticityandcognitivefunction.Neuropharmacology.2022Oct1;217:109191.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;021