guan-4-2 单克隆抗体.ppt

1、第4章-2，单克隆抗体单克隆抗体，单克隆抗体、抗体结构和功能，单克隆抗体准备和生产，单克隆抗体改造和应用，人力资源管理，中央循环管理Secondary lymphoid organs？Thymus、bone marrow tonsil、adenoid、lymphatic vessels、lymph nodes、spleen、peyers patches in small intestine、adenoid特征：外源性、结构性(分子表面有稳定的环状结构团作为识别点)、特异性(抗原和抗体反应)。antigens are molecules that react with antibodies，wh

2、ereas immuno gens are molecules that induce an immune responses . substances foreign Such抗原决定基免疫免疫免疫应答通常有多个徐璐不同抗原决定基，因此多个B淋巴细胞可以刺激相应的单克隆抗体的生成，因此血清的抗体称为徐璐不同抗原决定基的单克隆抗体混合物，多克隆抗体。类别、表格、对象之间的比率、时间、Antibodies that arise In an animal In response to typical antigens are hetero generous，Because they are for

3、med byfunctions neutralize toxins and virus opsonize microbes activate complements prevent attachment catalytic capability，Monoclonal antibody once activated by the occurres Classic examples are the antibodies to the child hood diseases chicken pox and measles . the second character istic of as ，it

4、is the first trait of antibodies，their specificity，That makes monoclonal antibody technology so valuable . not only can antibodiesthey can also help to diagnose a wide variety of illnesses，and can detect the presence of drugs，viral and bacterial products，And otherthere are two problems with this met

5、hod 3360 it yields anti serum that contains undesired substances，And it provides a very small amount of usable、structure and function of IgG、amino terminal end、carbody Variable regions。constantregions、fab fratgments、fc fragment、antibody、()Is not only the first class Of antibody to appear On the surv

6、er()Is the precip al class of antibody in secretions including saliva，tears，milk and the respiratory and intestine secretions。人体内5个单克隆抗体(McAb)，定义：抗体B淋巴细胞(antigen stimulated B lymphoblast)和具有无限增殖能力的骨髓瘤细胞(myeloma)仅针对特定抗原确定簇的牙齿单类抗体分子是单克隆抗体。、单抗制过程、动物免疫和亲本细胞的选择细胞融合：淋巴细胞杂交的制备杂交细胞筛选：有限稀释法等单克隆抗体制备及冷冻保存单克隆抗体

7、纯化； monoclonal antibody technology allows us to produce large amounts of pure antibodies in the following way 3360 We can obtain cells that produce Anting tumor cells that can replicate endlessly are fused with mammalian cells that produce an antibody . the result of this cell fusion is a hybridoma Which Which a myeloma is a tumor of the bone marrow that can be adapted to grow permanently in cell culture . when myeloma cells were fused with antibody-pre marrow and the ability to produce l