真核表达载体的构建

真核表达载体的构建Tag内容描述：<p>1、含报告基因的凋亡素基因真核表达载体构建研究 * 王福庆 1? 王庆宝2? 韩国新2 ( 1 . 滨州市人民医院, 山东 滨州 ? 256600 ; 2 . 泰山医学院, 山东 泰安?271016) 摘要: 目的? 构建含报告基因的凋亡素基因真核表达载体。方法? 对 pMD18T?VP3质粒进行 EcorI和 Ba mHI 双酶切, 回收 366 bp(含凋亡素基因完整序列 )片段。对增强型绿荧光蛋白 pEGFP?C2质粒酶切, 回收载体大片段。 将凋亡素基因通过连接反应, 连接到增强型绿荧光蛋白 ( EGFP)基因 C末端的终止密码前, 并使两者读框不移位, 构建成 pEGFP?VP3质粒, 并对其进行酶切分析和测序鉴定载体。</p><p>2、DNA甲基转移酶3A siRNA 真核表达载体的构建和鉴定【摘要】 目的:构建DNA甲基转移酶3A（DNA methyltransferases 3A,DNMT3A)siRNA重组质粒稳定表达载体，为进一步探讨DNMT3A在多种生物学过程及肿瘤发生发展中的机制提供工具。方法：依据DNMT3A基因的cDNA序列，利用siDESIGN软件获得RNAi靶位点后设计出对应的siRNA寡核苷酸模板，体外合成寡核苷酸片段经退火形成短双链，克隆到真核表达载体pSUPEREGFP1中，构建DNMT3A siRNA重组质粒载体，经酶切鉴定后转染人肝癌细胞系SMMC7721，荧光实时定量PCR初步分析DNMT3A经RNA干涉后的沉默效应。结果：。</p><p>3、三叶因子2基因真核表达载体的构建作者：张绍荣，杨晓强，邢锐，宋于刚，陈学清【关键词】 胃溃疡Construction of eukaryotic expression vector of human TFF2 gene【Abstract】 AIM: To construct a eukaryotic expression vector of human TFF2 gene. METHODS: We assembled a TFF2 gene in pfu mix reaction system and cloned it to pGMT easy vector. The positive colonies were first confirmed by restriction enzyme digestion and sequencing and then were inserted to eukaryotic expression vector pcDNA3.1 and verified by。</p><p>4、人cbl 基因真核表达载体的构建及表达【Abstract】AIM： To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1(+). The recombined vector was then transfected into COS7 cells with lipofectin and the expression of cbl gene was detecte。</p>