信息检索第五次作业、

1文献信息检索综合作业 3要求：1通过实习，巩固所学的检索策略及资源类型。2写出所用检索词、检索表达式及命中篇数并复制粘贴 2 篇在文挡中。3课堂作业评点和分析时，希望积极参与教学互动。一、医学数据库检索1、Pubmed 库（1）检索福建中医药大学吴锦忠教授发文情况，分别以题录（Summary）和文摘（Abstrats）格式下载保存。答案：检索式：Wu, JinzhongFull Author Name检索结果：3文摘方式：1. J Nat Med. 2013 Jan;67(1):17-26. doi: 10.1007/s11418-012-0639-x. Epub 2012 Mar 1.Investigation of the extracts from Bidens pilosa Linn. var. radiata Sch. Bip. forantioxidant activities and cytotoxicity against human tumor cells.Wu J, Wan Z, Yi J, Wu Y, Peng W, Wu J.Fujian University of Traditional Chinese Medicine, Fuzhou, Peoples Republic ofChina.The aim of this study was to evaluate antioxidant activities and cytotoxicityagainst human tumor cell lines of extracts from Bidens pilosa Linn. var. radiata Sch. Bip. (BP). Total phenolic and flavonoid contents of the extracts weredetermined by ultraviolet spectrophotometry. Furthermore, the antioxidant2properties of different polarity fractions extracted from BP were evaluated usingDPPH and ABTS radical scavenging test and FRAP assay. The ethyl acetate fraction (EE-BP) showed the highest antioxidant activity compared to other fractions. Inaddition, the anti-proliferative activities of the extracts on four human tumorcells, namely MCF-7, HepG2, MGC 803 and RKO, were investigated by MTT method. TheEE-BP displayed the most remarkable anti-proliferative effect against the tumorcells, particularly RKO cell in dose- and time-dependent manner. The antioxidant activities and cytotoxicity correlated highly with the total phenolic andflavonoid contents, respectively. Furthermore, The active ingredient BP-6, namely5,7,4-trihydroxy-3,3-dimethyl-flavonol, was isolated and purified with thepurity above 99.00% and content of 0.15% in EE-BP detected by HPLC, which couldsignificantly inhibit the proliferation of RKO cells with the IC(50) value of6.66 mol/l. In order to characterize the apoptotic RKO cells, flow cytometry andDNA fragmentation assay were performed. Apoptotic cell numbers increased in adose-dependent manner after the treatment with different concentrations of EE-BP and BP-6 for 12 and 6 h, respectively. DNA ladders in apoptotic RKO cells couldbe easily visualized when exposed to 200 g/ml of the EE-BP for 36 h. Takentogether, our work indicated that BP had potentially therapeutic value againstcolorectal cancer.PMID: 22382861 PubMed - indexed for MEDLINE2. Curr Microbiol. 2011 Apr;62(4):1160-7. doi: 10.1007/s00284-010-9838-4. Epub 2010 Dec 15.Differential proteomic analysis of temperature-induced autolysis in mycelium ofPleurotus tuber-regium.Huang B, Lin W, Cheung PC, Wu J.School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou350002, China.Autolysis is an important physiological process found in fungal cultivation.However, there is hitherto no report on the autolysis of Pleurotus tuber-regium. We have investigated the enzymes secreted by temperature-induced (40C astreatment versus 10C as control) autolysis of the mycelium of P. tuber-regiumgrown in submerged cultivation. A comparison between the intracellular proteins(inside the mycelium) and the extracellular proteins (in the culture medium) ofthe treatment and control by proteomic analysis involving 2D PAGE andMALDI-TOF-MS was made. Twenty-two up-regulated protein spots were detected andeight proteins were identified. They included proteasome which participates inthe ubiquitin-proteasome pathway; -1,3-glucanosyltransferase and tubulin which3are involved in the renewal and repair of cell wall; protease and endoglucanasewhich promote the natural degradation of cell wall and cytoplasm; 14-3-3 protein which takes part in cell signal transduction; and two putative proteinspresumably relate to the autolysis process. These identified proteins suggestpartially the metabolic processes of the autolysis in the P. tuber-regiummycelium.PMID: 21161227 PubMed - indexed for MEDLINE3. Zhongguo Zhong Yao Za Zhi. 2010 Sep;35(17):2315-7.Determination of protopine in Corydalis racemose by HPLC.Article in ChineseJiang X, Ye J, Zeng J, Zou X, Wu J.Fujian Academy of Integrative Medicine, Fuzhou 350108, China.OBJECTIVE: To develop a HPLC method for determining the content of protopine inCorydalis racemose.METHOD: Analysis was performed on a Gemini C18 column (4.6 mm x 250 mm, 5 microm)eluted with acetonitrile-water containing 0.8% triethylamine and 3% acetic acidacetum (20:80) as the mobile phase. The flow rate was 1.0 mL x min(-1). Thedetection wavelength was 289 nm.RESULT: The average content of protopine in Herb of Racemose Corydalis was0.905%. The calibration curve of protopine was linear between 0.124-1.36 microg(r = 0.9999). The average recovery was 98.49% with RSD 1.9%.CONCLUSION: This method is simple, reproducible and can be used to determine the content of protopine in C. racemose.PMID: 21137346 PubMed - indexed for MEDLINE以题录下载：1: Wu J, Wan Z, Yi J, Wu Y, Peng W, Wu J. Investigation of the extracts fromBidens pilosa Linn. var. radiata Sch. Bip. for antioxidant activities andcytotoxicity against human tumor cells. J Nat Med. 2013 Jan;67(1):17-26. doi:10.1007/s11418-012-0639-x. Epub 2012 Mar 1. PubMed PMID: 22382861.2: Huang B, Lin W, Cheung PC, Wu J. Differential proteomic analysis oftemperature-induced autolysis in mycelium of Pleurotus tuber-regium. CurrMicrobiol. 2011 Apr;62(4):1160-7. doi: 10.1007/s00284-010-9838-4. Epub 2010 Dec415. PubMed PMID: 21161227.3: Jiang X, Ye J, Zeng J, Zou X, Wu J. Determination of protopine in Corydalisracemose by HPLC. Zhongguo Zhong Yao Za Zhi. 2010 Sep;35(17):2315-7. Chinese.PubMed PMID: 21137346.（2）检索多动症病因及饮食治疗方面的文献。 （利用MeSH 检索）2、西文生物医学期刊文献数据库 P102检索肝癌病人护理方面的文献。并告知获取外文全文的渠道。检索词 检索表达式 检索结果肝癌、护理 肝癌 and 护理 1497Liver cancer care 全部=liver cancer care5446获取途径： DOI PubMed 全文链接及在线提交请求信息 Google 全文通道3、万方医学网检索“冠状动脉硬化发病与流行情况”的相关文献（分别用关键词检索与 MeSH 检索途径，看检索结果有何不同） 。P108若是用关键词检索，则检索结果为 054、中医药在线检索“老年痴呆的针灸疗法的研究文献” 。 （分别用主题词检索和关键词检索，看检索结果的不同）5、CMCC 数据库检索福建中医药大学或你大学所在学校被国家自然科学基金资助所发表的论文情况。二、引文检索利用中国引文数据库（CNKI） 、CNKI 中心网站上的“引文检索” 、万方引文库（嵌入在论文检索中） 、维普中心网站上的“文献引证追踪” 、Google 学术搜索（嵌入在论文检索中） 、读秀知识库、SCI 数据库，检索黄老师或者你的专业老师论文总被引的次数。以题录格式列出。格式：（1）被引文献：张予阳，崔连静，孙文静，程佳艺，唐星. 冠心苏合丸与冠心苏合软胶囊药理作用的比较J. 中药药理与临床，2000，16(5)：6-7.引用文献：61 吴海燕 ,春香, 由会玲, 李国川, 郝蕾 RP-HPLC 法测定新冠心苏合滴丸中肉桂酸含量的实验研究河北医科大学学报 2008,29(2). 2 史保权,李春香, 丁里玉, 郝蕾,丁芳. 薄层色谱法鉴定新冠心苏合滴丸中苏合香武警医学院学报 2009,18(8). 。 。 。 。 。 。被引文献：检索入口检索结果 被引次数CNKI 被引文献：叶玲，郑鸣霄，陈鸿。超声诊断在肛周脓肿、肛瘘的临床应用，福建中医学院学报，2003,47叶玲，郑鸣霄，陈鸿。超声诊断在肛周脓肿、肛瘘的临床应用，福建中医学院学报， 2003 年 13 卷 02期 11-12 页32 叶玲，郑鸣霄，陈鸿。应用超声技术诊断肛周脓肿肛瘘 98 例，中医杂志 2003 年 44 卷 z1 期 13 叶玲 蓝宗毅 高献明 赖日昌 . 紫芨油应用于湿热下注型混合痔术后 120 例临床观察。中国现代药物应用 2013 年 7 卷 14 期 130-131 页 04 叶玲 . 应用超声技术诊断肛周脓肿肛瘘 98 例全国第十一次大肠肛门病学术研讨会 2003 年0万方5 柯敏辉 叶玲 郑鸣霄 . 消痔灵注射辅以中药薰洗治疗脱肛病 30 例临床研究。世界中医药 2012 年 07 卷05 期 392-394 页0维普 超声诊断在肛周脓肿、肛瘘的临床应用 4应用超声技术诊断肛周脓肿肛瘘 98 例 17电脑肛肠检查治疗系统 610 例临床诊断分析 0三、专利检索（国内可用数据库：国家知识产权局专利库；万方数据库专利检索；CNKI 专利检索；中国专利信息网国外可用数据库：美国专利数据库；欧洲专利数据库）检索：利用中外专利各一种，查找你的专业相关专利文献分别检中多少？（写出你所用的关键词）检索入口 检索内容 检索表达式检索结果国家知识产权局专利库/zljs/肛肠 发明专利 101实用新型专利292外观设计 162万方数据库专利检索/肛肠 检索表达式：题名或关键词:(肛肠) * Date:-2013医生、医院和实验室用的固定器械和设备(98)8CNKI 专利检索（cnki 中国专利数据库）/grid2008/Unis/html/scpd/zlkjj.htm在专利高级检索，勾选医药卫生科技，输入肛肠专利名称=肛肠416中国专利信息网/国内肛肠国外