DNA提取实验报告

dna dnadnadnadnadnadnadna 1lblb2lb37(200/) dna()11.5ml 12000rpm302 3 100li54 200liidna5105 150liiiiii56 21/1010dna412000rpm10min7 1ml 708dna51091010 34lteph8.0-20dna1 eppendorf 0.5mldnal102l0.5l2 eppendorf372-33 2l0.1mol/l edtaph8.0 dna1 5tbe100ml1000ml0.5tbe2 3g1000ml300ml 0.5tbe1%3 50-60eb0.5lg/ml0.5tbe4 20l2l10tip5 60-80v40ma2cm6 rnadnarnadnarna1 2 3 4 071 20073305dnadnadnadna1. 2. 1. 1mol/l tris.cl(ph8.0) 12.11g tris80 mlddh20,4.2 ml,ph8.0100 ml2. 0.5 mol/l edta(ph8.0)18.61g na2edta2h2o80 mlddh2o,naohph8.02 g100 ml3. 100 mmol/l tris.cl(ph8.0), 20 mmol/ledta, 500 mmol/l nacl,1. 5%sds4. 80/4/165. 6?loading buffer0.150.15ff5 mmol/l edta501. 15ml5ml602. 1-2g6020 min3. 5000 rpm54. 5 ml /5-105. 5000 rpm56. 7. 5000 rpm5 min8. 1mlddh2o60159. dna5 l1 l6?loading buffer0.7%dna410231dna210120100107011dna _ dna 1dna 2 3dna 4 5dna dnactab) (sds)dna(dnarna)dnadnate dnadnadnactab-dnadnasds,dna,dna()dna() (sds)dnadna dnadnadna:dnadnadnadnadnaebdnadnadnarna260nm260nm260nmdnarna1g / mldnaod2600.02od2601dna50g / mldna37g / mlrna40g / ml30g / ml1dnarna1cm h2odnarnanh2ood260dnadnag/l=50od260 dna/1000rnag/l=40od260 rna/1000dnadna dna260nm280nm230nmod260od280od2302 dnaod260/od2801.8dnaod260/od280 1.9rnaod260/od280 1.6 rna1.7 od260/od2802.0rnaod260/od280 1.7od260/od280 2.0 od230/od2600.4-0.5pcr121dna2:3te45rnaa6/7/870%9 3mol/l naac10 5tbe11 612 1.0%13 eb14 dnamarkers1255642027232243616557941623130bp.15 ddh2o127ml5ml310ul200ul1000ul465 567 37891011120.5cm1cm50ul100uldna1101g250ml100ml 0.5tbe5060eb0.5mg/l0.5tbeeb25uldna1ul 6dna3100v2cm3060min4dna dnadna20ul5mlddh2o teph 8.01000.5cm1.6ml230nm260nm280nmdnadnadnadna1dnadnadnag/l =959.328ng/ul2dnadnaod260/od2801.97od230/od2602.073 od260/od280 1.8dna od260/od280 1.9rna od260/od280 1.6od260/od2801.97rnadna dnadnadna dnadnadnadna 12 5v/cm 0.51.0v/cm ebebdna :taetbetpetaetpednadnatbeedtadnasednaph : dna1dna2dnasednasednaabphph8.0cdedtamg2+3dnaph8.04dnadna5dnadnarnadnarnadna1210dnapcrpcrdna 1131428 1pcr2dna1. pcrpcrdnadnadntptaqdnadna9455dna72tapdntpdnadnadnadnadnadnadna3040dna2. dnadnadnadnadnadnapcr0.2ul1.5ml tapdnadntpbuffer16sdnaddh2odna tbeeb 1. pcr16s rdna v31.1. 1.5ml2.5ul 10buffer 1 ul dntp0.5 ul 341gc0.5 ul 5340.125 ul taq19.375u ddh2opcr1.2. 924 ul89ddh2o1.3. pcrpcr3040 94 3min 94 30s55 30s72 30s72 5min1.4. pcr152. dna3. 10.2g0.5tbe60eb2.2 602.3 2.4 0.5tbe1l5ldna2.5 5v/cm2.6 1cm2.7 1-898200bp9marker100bp11. pcrdnapcrpcr2. dna3. 4. eb1. pcr2. dna1pcrpcr3. 5-gactccagtcgaatctacca-35-aaccgtggcgacaccgctaatmpcr5-gactccagtcgaatctacca-3tm=4g+c+2(a+t)-(51