《分子生物学习题》PPT课件.ppt

名词解释 1. Chromosome 2. Nucleosome 3. Overlapping gene 4. Split gene 5. Gene family 6. Pseudogene 7. Melting temperature 8. Gene superfamily 9. Replication 10. Semi-conservative replication 11. Okazaki fragment 12. Semi-discontinuous replication 13. DNA repair 14. DNA recombination 15. DNA transposition 16. Transposon 17. Replicon 18. Monocistron 19. Polycistron 20. Replisome 21. Retrotransposon 问答题 1、真核生物基因组中的DNA重复序列主要有哪些类型？简要说明基因 组重复序列可能的生物学意义以及基因组重复序列在分子标记研究中 的应用。 2、试述保证DNA复制精确性的机理。 3、什么是DNA双螺旋结构？ 4、比较原核生物、真核生物及病毒基因组之间的异同。 5、两个等长的DNA片段，一个GC含量为70%，一个GC含量为30%， 哪个TM值高？为什么？ 6、说说DNA损伤与DNA突变之间的区别和相互关系。 7、原核生物与真核生物存在哪些类型的转座子？转座机理有哪些？ 8、转座有哪些遗传学效应？ 1. What is replicon? How many origins and termini shall a replicon contain? 2. What is semi-conservative replication? 3. Please list at least three main difference between bacterial DNA replication and eukaryotic DNA replication. 4. Please define the term “mutation”, and list the major types of DNA mutations and their corresponding phenotypic effects. 5. Please define the term “DNA lesion”. Please list the three types of DNA lesions and the chemical or physical mutagens cause these lesions. 6. Will all the DNA lesions result in mutations? Why and How? 7. What are the three main DNA recombination technologies that the cells can apply to change their genetic materials? 8. How is the high fidelity of DNA replication ensured? 9. Suppose that you would like to introduce mutations in E. coli genome. What would happen in cells if you use low dose of UV light? How about high dose? How about using base anolog 5