imagej的使用(分析荧光图片).ppt

Gao yan ImageJ Features nRuns Everywhere: nImageJ is written in Java, which allows it to run on Linux, Mac OS X and Windows, in both 32-bit and 64-bit modes. nOpen Source: nImageJ and its Java source code are freely available and in the public domain. No license is required. nPlugins: nExtend ImageJ by developing plugins using ImageJs built in text editor and Java compiler. More than 500 plugins are available. nAnalysis: nMeasure area, mean, standard deviation, min and max of selection or entire image. Measure lengths and angles. Use real world measurement units such as millimeters. Calibrate using density standards. Generate histograms and profile plots. Installation nLinux nMac OS X nMac OS 9 nWindows Windows /ij/download.html Download ImageJ 1.44 bundled with 32-bit Java 1.6.0_20 (28MB), with 64- bit Java 1.6.0_20 (24MB; requires 64- bit Windows) or without Java (3MB). Open Reads an image and displays it in a separate window. Files must be in TIFF, GIF, JPEG, DICOM, BMP, PGM or FITS format. Also opens ImageJ and NIH Image lookup tables (with “.lut“ extension). Additional file formats are supported via plugins installed in the Import submenu. Subtract background Merge channel Scale bar nScale bars should be present on all publication/presentation images/movies. Its worth putting them in sooner rather than later. Choose a standard scale bar size for all your images if possible to avoid confusion. n If you know the size of a feature (previously applied scale bar for instance) you can use this command to apply a calibration. nUsing the line selection tool, draw a ling along the length of the feature/scale bar. nRun the menu command “Plugins /Spatial calibration/Set scale”1. nEnter the dimensions of the object/scale bar in the “known distance” box and set the units in the Unit of length box. nDo not check Global unless you wish all your images to have this calibration! Click OK. n Analyze Particles and count cell nThis command counts and measures objects in binary or thresholded images. It works by scanning the image or selection until it finds the edge of an object. It then outlines the object using the wand tool, measures it using the Measure command, fills it to make it invisible, then resumes scanning until it reaches the end of the image or selection. Press t