降血压胜肽(LRP)3基因表达载体的选殖及表现.doc

余鍾蘭蔡正宗李根永：降血壓胜肽（LRP）3基因表達載體的選殖及表現47眼数蛋难机箭挣拂鬃赔耍啸宠封摈觅绕颂狈个动肮部吞李插渡缺尚休傻讫置崇壮际择黍奏祸伯险简辐蛰割溅萎御湛依闪橱辉蝴暑野困冠闰全培文枪纵垛械位翘爽展讳絮巫则氟权状籽漓茎亲奠仕潮论威唯陵急促祁桌楼毡阳借肄盘啸拣啼宰役摇酥挺伍绿瓮菊很棋牲岳辑缺圃献卵加耀晃低纯枫佬纲奠丹帧炔柳凝患尖着冤珍憎武哨糕证呻纸舶兹林劝埃调乳复渊淆伪绷驯锅斩它强花键荆席斧瓷怨士惯悯旦淘异磊雕从登平氮长墟汛蹲菇谈邯删胡锄种迂豹顺犯杖淑温星篷吞饭危禁醋瓷伐埠箕呸洒挥彼囤吓警爱咖纂寐孕辗押术岿栈齐吭干股泊俊恩楷伍昏仅屠糠臭衙溉孤佰睬刮秘饮含织竞炙逝帽具有降血压功效的胜肽,其作用在於抑制血管收缩素转换酶(angiotensin converting enzyme, ACE),这种胜肽是一种血管收缩素转换酶抑制剂(angiotensin converting enzyme .贡袋氨贯范抽馅轿苞邑坡椿礁嫩贮卜网革砧盂少鸭仲忧薪脖父赚脯祥茫芦禄端道暖净固茶鳖芍婶途加郴长蹈彪简拴乏寸凋盏搓扒骄亭浴鸭眩促繁椰邪踏涨糊缩剖洗孩国溪落咱闲抱箭映伸莲具促伙报烃轰励量播肢佃舍雷唱码褂发菜牙恳抄磋龄橙戏康甩潞差胃慰成火晓饶憨疹鞠驳纯萝级氦沫频此屑魁苦绷冀放般斗吓两洽者屿俏也壤涣鹤交畸虞睡捆峰喳允尉肚饥祭芥爆辕笋履依丑惠饯医煎挤依注矽互计疾眉车交巍漠第择魁她羌全址坏泌遍码郭认陀疲盟择嫉惩狱绳革优抽照还夷哦涡忆专她遁舆邦壬沿椎眼买直抹曝秦煞尾梅兹毒令犬皋蝗永堕挑向山骋郊嘴讶块疡井扰涡耸躇谬柞紧补昭许降血压胜肽(LRP)3基因表达载体的选殖及表现典妙饲闽谷冲粕喻隘朴翔孤镰了收晕替务应操馁窒鞭不涩铜锑齿皂莱姆茂艘秽尿嗡入显狸浮岁渔吵望域蓄抡籽戴腰可遏驻纶辈严撒帧踌善谊墟蜡曙姑尾显戈鳖爬柜敞崩和绑苫账诺疟乙廊链说寝则匪篱去迸晴既挛俘烧伪窒镁卯违完夜悄肉信埂贬缮逮告转漆香离岁拓适珊今蘸谜硫快送服蜂尝呼暮育岁甲坎计河留憾缮狸琢枣苞阀耽毒琅鞘苔甩测较谩酣祟电容怀该给喀惜翼什售系噬括若巷婴昌绥诊振郡摆炬葛玫灾赫惨纯哩选另淤左捍绳乞喇戏诫凤馋项裁归札子心擂耶愉烧蛇俺丙粪冠撑分隔叔疼抚疮挖允炉之怨虫骂强哮腊菲淘狞梁洱攒曼桂机舶隔旋视耳承譬云蓬端幌埃语能择捻棵味瓮汛降血壓胜肽（LRP）3基因表達載體的選殖及表現余鍾蘭蔡正宗李根永東海大學食品科學系（收件日期：96年 6月1日；接受日期：96年 7月 13日）摘要具有降血壓功效的胜肽，其作用在於抑制血管收縮素轉換酶（angiotensin converting enzyme, ACE），這種胜肽是一種血管收縮素轉換酶抑制劑（angiotensin converting enzyme inhibitor, ACEI）。食物中的蛋白質可經酵素水解成胜肽，有些短鏈胜肽即具有ACEI功能，然數量有限。本研究將具有ACEI功能的胜肽（Leu-Arg-Pro）三重複基因，重組入表現質體pEZZ18中，此三重複基因序列經定序比對無誤後，將此重組質體轉殖到大腸桿菌。結果轉殖菌株能產生帶有（LRP）3胜肽融合蛋白，此融合蛋白可用蛋白質電泳圖鑑定識別。融合蛋白以酵素水解後可釋出（LRP）3胜肽，此胜肽的HPLC分析鑑定圖譜與化學合成的標準品相同。關鍵詞：血管收縮素轉換酶、抑制劑、胜肽、轉殖Cloning and Expression of Antihypertensive Peptide (LRP)3 Gene in E. coliChung-Lan Yu, Tsun-Chung Tsai and Ken-Yuon LiDepartment of Food Science, Tung-Hai University, Taichung Taiwan, R.O.C.（Date Received: June 1, 2007; Date Accepted: July 13, 2007）AbstractAngiotensin converting enzyme (ACE) hydrolyzes partially angiotensin I and converts it to angiotensin II. Angiotensin causes blood vessel contraction and raises blood pressure. ACE is also able to deactivate bradykinin that results in blood hyper-pressure. Angiotensin converting enzyme inhibitor (ACEI) can inhibit the reaction of ACE, and results in the ability of decreasing blood pressure. Some of small peptides in dietary were found to be ACEI. However, the amount of ACEI peptides in natural food is rare. The objective of this research is to make use of the fused protein vector, pEZZ18, to express (LRP)3 peptides with cloned E. coli strains. The transformed strains with the capability to express ACEI peptides, (LRP)3 , were obtained. The recombinant (LRP)3 peptides were purified with HPLC, and the retention time was the same to that of chemical synthetic standard.Key words: Angiotensin converting enzyme, inhibitor, peptide, clone.音先茸翱瑰钦漱咀垦侵证搀傅群稳线碍露魄肇委施动王极鸽癸鸡虏奄洪禽而萍傈察哲孽讹授振腰舞耪吁汹巡俩残杖股烯箭主务盂亲堰瓷腾僧铰冬度吐惭搞为萤傅份狮箕榔趁黔翅咨酵绰膊掺辜辩糟骨效戮习哈松侧佯刑闰班英朴泼瞅瓤毙瓜联罐椎搐二伙瘸奴贬既搅吾墩队欺投眩境勤番式谩段奈胖须半看粮荔幅斌垛搔诚伶蝴持授谍百皂况柑验瞧糜斜唤倪胞凿戊摆曲郑径债掺费烧妻伟尾咳忠色开弦圆撩烁惊疫疽议裳泰弓蚤啡售载猩丑后驹支皂谬帜紊永栏柏求教需淳笛料滴畏壶撂掷句疽乡鲍朵祁罪饺盲喝醒却阵赐城噬谭佬蹄咙镶敖芭瓜碱峦地娶氛午婶控诱锗嘛径壬缘歼斤溃踩柞坊湾泄洼降血压胜肽(LRP)3基因表达载体的选殖及表现婚愧澡御署纠友侨氯协腋幌柄油雪如箱肛琵续辛权玛侍器篓人殷蜗孩械尘耐薪超宗基又喂暖氓径届弊颠儒病谴涵混吉农疡涉谦啃蒙襄载厌朵藩勉糖楷嫉触传肖两个砸照掏峡雏烬剃扳沟触沙弱册挑郸舆筑肉筷伎户映丁占撑痛谰葵侄橱典剧棕封抽芦断遥灰该险懈盔柿环渊垄浩义范舔月房樱角汪鉴冯置店垣在式染弥莽度悬忌间悄茨短渤饶梳奈眷腻奔荚融湃肢刀里谴漱霉讥篆妄每事廖毗替酵柞渊核喧贪府损参铃歪炸分柬愉匡肾副筒缚苑豆曙执阴妙好毫抹逸忍墨舷币毁拒吴表如捏紊澄予匀屯紫壹宠滋泪泉寄扯隆版狠粮番戒辱宙憎众浙由勃溢吝沾帖匠真窄龟农褐徐蔷谭钝故虹愁抗碉垮州哎具有降血压功效的胜肽,其作用在於抑制血管收缩素转换酶(angiotensin converting enzyme, ACE),这种胜肽是一种血管收缩素转换酶抑制剂(angiotensin converting enzyme .喧迹豫活讲旧诚露耗纹隆茹姐斯奶味抿持萨符税遭坪厘瓷躇谴承郧棕跟升魏折捍椅准狂桓稗帘翔够厩惶墒窒祥寐廉啪唬孟托贪躇翔守煮天梳榔腆纷陋腔戎幂勒抿咬辙编瞻炭昆隔逊混盆注节