运动论文：不同运动训练时间大鼠心肌t-mTOR和p-mTOR蛋白的表达.doc

运动论文：不同运动训练时间大鼠心肌t-mTOR和p-mTOR蛋白的表达【中文摘要】:通过大鼠运动训练后心肌t-mTOR和p-mTOR蛋白表达的变化,来探讨它们在运动诱发心肌功能变化过程中的作用以及运动对mTOR信号通路的影响。方法:急性组运动模型:选用雄性清洁级SD大鼠50只,随即分为安静组11只和一天急性训练组(运动后即刻13只、运动后3小时13只、运动后24小时13只),安静组不运动,只进行正常的笼内生活,大鼠适应实验环境3天后开始训练,运动组参照Bedford渐增负荷跑台运动模型训练。一个月组运动模型:雄性清洁级SD大鼠50只,随即分为安静组11只和一个月训练组(运动后即刻13只、运动后3小时13只、运动后24小时13只),安静组不运动,只进行正常的笼内生活,大鼠适应实验环境3天后开始训练,参照Bedford方法和王蕴红的长期递增负荷耐力训练建立运动模型,采用跑台方式训练。三个月组运动模型:雄性清洁级SD大鼠50只,大鼠适应环境后随机分为安静组11只和耐力训练组(运动后即刻13只、运动后3小时13只、运动后24小时13只),大鼠适应实验环境3天后开始训练,参照Bedford方法和王蕴红的长期递增负荷耐力训练建立运动模型,采用跑台方式训练。大鼠每周训练六天,周日休息,共训练10周,具体的训练方案是:安静对照组大鼠为正常笼内饲养,不进行任何运动。运动组大鼠每周周一至周六运动,周日休息。每周一开始运动之前称体重并记录。末次运动后按实验设计的各个时间点取材。取大鼠左心室心尖。然后收集蛋白样品(研磨,提蛋白,测蛋白,调蛋白,煮蛋白,分装),电泳,转膜,封闭,一抗孵育,二抗孵育,蛋白检测。所得数据用SPSS13.0统计软件分析。结果:急性一天训练组大鼠t-mTOR和p-mTOR在大鼠左心室心肌的表达安静组与运动组表达没有规律性变化。一个月训练组大鼠t-mTOR和p-mTOR在大鼠左心室心肌的表达情况为:运动后3小时,运动后24小时与安静对照组相比表达升高,差异不显著。三个月训练组大鼠t-mTOR和p-mTOR在大鼠左心室心肌的表达情况为:运动后3小时,运动后24小时与安静对照组相比表达升高,差异显著。结论:1急性运动后大鼠左心室心肌t-mTOR和p-mTOR蛋白表达没有显著变化。2一个月训练组大鼠t-mTOR和p-mTOR在大鼠左心室心肌的表达情况为:运动后3小时,运动后24小时与安静对照组比表达升高但没有显著性差异。表明一个月训练后也没有引起t-mTOR和p-mTOR蛋白的显著变化。3三个月训练组大鼠t-mTOR和p-mTOR在大鼠左心室心肌的表达为:运动后3小时,运动后24小时与安静对照组相比表达升高,差异显著。运动恢复期蛋白合成增加。表明mTOR信号通路与心肌肥大密切相关。mTOR信号通路可能参与了心肌功能的调节和形态的变化。【英文摘要】: to explore the role of exercise in my ocardial function in the process, as well as the impace of the exercise,we observe the expression of the member of t- mTOR p-mTOR.Methods: Model of acute training:we selected 50 male clean SD rats for actute exercise and they were randomly divided into a control group and acute exercise (instantly after exercise, control ,3 hour, 24 hours after exercise), the rats control group just lived a normal life in cafe in cage and dont exercise, after 3 days adaption,the actute exercise training group awere trained on the treadmill which refered to the Bedford increasing load model.Model of a month exercise group: were randomly divided into a control group and Group of a month exerciseing (instantly after exercise, control,3 hour, 24 hours after exercise),in 50 SD male rats. the rats control group just lived a normal life in cafe in cage and dont exercise, after 3 days adaption,the exercise training groups were trained on the treadmill which refered to the Bedford and wangyunhong increasing load model.Model of 3 month exercise group: were randomly divided into a control group and Group of a month exerciseing (instantly after exercise, control,3 hour, 24 hours after exercise),in 50 SD male rats. the rats control group just lived a normal life in cafe in cage and dont exercise, after 3 days adaption,the exercise training groups were trained on the treadmill which refered to the Bedford and wangyunhong increasing load model. Big mouse each week trains six days resting at Sunday, training 10 weeks. the concrete training plan is: the rats control group just lived a normal life in cafe in cage and dont exercise, trains group exerciseing on monday to Saturday, resting on Sunday.we record the body weight on Every Monday.The rats were killed after the last trainging according to the schedual time.following we took out Apex cordis of Left ventricle in the heart. Then the collection protein sample (attrition, raises protein, measures protein, adjusts protein, boils protein, subpackage), the electrophoresis, transfers the membrane, the seal, a anti-foster, two anti-fosters, protein examination.The obtained data counts the software analysis with SPSS13.0.Result: in acute training the expression of t-mTOR and p-mTOR does not have the change on myocardial.A month training group the expression of t-mTOR and p-mTOR on myocardial :the express increased in 3 hours,24 hours after exercise compared with control group. The difference is not remarkable.3 months training group the expression of t- mTOR and p-mTOR on myocardial :the express increased in 3 hours, 24 hours after exercise compared with control group. The difference is remarkable.Conclusion:1 in acute training the expression of t-mTOR and p-mTOR does not have the regular change on myocardial. it indicated mTOR signal passage is not remarkable on myocardial in acute training.2 A month training group the express increased in 3 hours, 24 hours after exercise compared with control group.the express increased in 3 hours, 24 hours after exercise compared with control group. The difference is not remarkable. A month training that did not cause the change of t-mTOR and p-mTOR protein.3 3 months training group the expression of t-mTOR and p-mTOR on myocardial :the express increased in 3 hours, 24 hours after exercise compared with control group. The difference is remarkable.mTOR signaling pathways closely related with myocardial hypertrophy.mTOR signaling pathways may be involved in the regulation and morphological change of myocardium function.【关键词】运动 心肌肥大 t-mTOR p-mTOR【英文关键词】exercise cardiac hypertrophy t-mTOR p-mTOR【目录】不同运动训练时间大鼠心肌t-mTOR和p-mTOR蛋白的表达中文摘要5-7Abstract7-81 前言9-171.1 选题依据9-101.2 文献综述10-171.2.1 mTOR 的分子结构及其复合物11-141.2.2 mTOR 的信号传导通路14-161.2.3 运动对mTOR 信号的影响16-172 研究方法17-212.1 研究对象17-182.1.1 实验动物及分组172.1.2 实验动物饲养17-182.2 动物模型182.2.1 急性组运动模型182.2.2 一个月组运动模型182.2.3 三个月组运动模型182.3 实验动物取材18-192.4 测试指标19-202.4.1 心系数192.4.2 Western blot 测定mTOR 和p-m TOR 蛋白的表达19-202.4.3 数据采集和统计处理202.5 实验仪器及试剂20-213 研究结果21-303.1 心系数21-243.1.1 急性组心系数的变化21-223.1.2 一个月训练组心系数的变化22-233.1.3 三个月训练组心系数的变化23-243.2 运动训练中t-mTOR 表达的变化24-273.2.1 急性组t-mTOR 表达的变化24-253.2.2 一个月训练组t-mTOR 表达的变化25-