M29基因在番茄花序及果实发育中的作用.pptx

Down-Regulation of TM29, a Tomato SEPALLATA Homolog, Causes Parthenocarpic Fruit Development and Floral Reversion,Charles Ampomah-Dwamena, Bret A. Morris2, Paul Sutherland, Bruce Veit3, and Jia-Long Yao,Zhang Yunyi 09/06/16,SEP functions in Arabidopsis Sequence analysis of TM29 Spatial and temporal expression of TM29 in tomato TM29 transgenic plant phenotypes TM29 protein interacts with SlHDACs Conclusion References,Context,SEP functions in Arabidopsis,B and C floral organ identity functions require SEPALLATA MADS-box genes Pelaz et al., Nature 405, 200-203(11 May 2000),SEP functions in Arabidopsis Sequence analysis of TM29 Spatial and temporal expression of TM29 in tomato TM29 transgenic plant phenotypes TM29 protein interacts with SlHDACs Conclusion References,Context,TM29 Groups to the SEP Subfamily,Phylogenetic analysis,Sequence analysis,SEP functions in Arabidopsis Sequence analysis of TM29 Spatial and temporal expression of TM29 in tomato TM29 transgenic plant phenotypes TM29 protein interacts with SlHDACs Conclusion References,Context,Spatial and Temporal Expression of TM29 in Tomato,fb, Flower buds fr, Young fruits lf, Young leaves sh, Shoot tips rt, Roots,Northern-blot hybridization,pa, Pre-anthesis a, Anthesis DPA, Fruit at 3, 6, 14, and 21 days post-anthesis lf, Young leaves,TM29 expression occurs from the early stages of flower development to the early stages of fruit development.,Spatial and Temporal Expression of TM29 in Tomato,Sympodial bud,Floral(fm) /Inflorescence(im) meristem,Sepal,Ovary,In situ hybridization,sb, sympodial bud; lf, leaf; fm, floral meristem; im, inflorescence meristem; vb, vascular bundle;,se, sepal primordium,an, anther region; ov, ovary primordium; per, pericarp region; st, stamen; tap, tapetal region;,SEP functions in Arabidopsis Sequence analysis of TM29 Spatial and temporal expression of TM29 in tomato TM29 transgenic plant phenotypes TM29 protein interacts with SlHDACs Conclusion References,Context,TM29 Transgenic Plants Phenotypes,Sepallata-like flowers Delayed senescence,petal,stamen,TM29 Transgenic Plants Phenotypes,Delayed ripening process Ectopic shoots grew inside the furit,TM29 Transgenic Plants Phenotypes,el, Ectopic leaf; ef, Ectopic flower; fr, Fruit;,es, Ectopic inflorescence; ov, ovule; per, Pericarp; pl, Placenta; sd, Seed,TM29 may be required for the maintenance of floral meristem identify,SEP functions in Arabidopsis Sequence analysis of TM29 Spatial and temporal expression of TM29 in tomato TM29 transgenic plant phenotypes TM29 protein interacts with SlHDACs Conclusion References,Context,v,TM29 protein interacts with SlHDACs,SlHDACs= Solanum lycopersicum Histone Deacetylases,Yeast two-hybrid assay,Pull-down assay,SEP functions in Arabidopsis Sequence analysis of TM29 Spatial and temporal expression of TM29 in tomato TM29 transgenic plant phenotypes TM29 protein interacts with SlHDACs Conclusion References,Context,TM29 shares a high amino acid sequence homology to the Arabidopsis SEP1, 2, and 3 genes TM29 is involved in floral organs development like SEP genes TM29 may also play a role in the maintenance of floral meristem identity and fruit development in tomato TM29 protein may recruit HDACs to regulate gene expression in reproductive development in tomato,Conclusion,SEP functions in Arabidopsis Sequence analysis of TM29 Spatial and temporal expression of TM29 in tomato