Serva蛋白质电泳产品.doc

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SERVALYTTM两性电解质载体 (40% W/V)两性电解质是低分子量的两性离子体。他们是合成的并可组成一系列变化的等电点。在琼脂糖和聚丙烯酰胺凝胶中含有两性电解质，跑电泳时会形成线性pH梯度。两性电解质分子带有净电荷，可以在电极间的电场移动直到到达相应的等电点位置，然后停止移动并形成固定的平台。SERVALYTTM是一种合成混合物，平均分子量在400到1000道尔顿之间。具有如下优点：l 由多种聚合体构成而得到的高分离效果l 可以在短时间内染色或脱色l 清晰的背景，染料的非特异结合非常低l 在三氯乙酸中具有很高的溶解度（固定时迅速去除两性电解质） 为了使等电聚焦达到良好的分离效果，需要稳定的pH梯度并且具有广泛并恒定的缓冲能力。由不同等电点的低分子量物质组成的良好的两性电解质混合物在等电点具有良好的传导性。如果能够维持两性电解质的成分，每次实验都能得到可靠的结果并具有良好的可重复性。Serva能够提供以下三类两性电解质：宽pH值：跨度超过3个pH单位（例如SERVALYTTM3-10）窄pH值：跨度为2-3个pH单位（例如SERVALYTTM3-6）超窄pH值：跨度为1个pH单位（例如SERVALYTTM3-4）SERVALYTTM两性电解质载体为40%的水浓缩物，过滤消毒（0.2mm）。通常的工作浓度在3-5%。在4度条件下，未打开的SERVALYTTM可以储存长达三年。T级别的SERVALYTTM两性电解质载体没有过滤消毒，280nm的UV吸收高于分析（analytical）级别的SERVALYTTM两性电解质载体，边缘的缓冲能力也低。SERVALYTTM3-10 IsoDalt （cat. 42951）尤其适于2D-电泳。宽和窄pH值的SERVALYTTM是可以立即使用的。通常不需要与其他pH组分混合。但是有一些例外也需要注意。非常酸（pH2-4）或者非常碱（pH7-9/9-11）可能在聚合时引起问题。我们推荐加入20%SERVALYTTM3-10（cat.42940）。在倒胶前将8ml的酸或者碱SERVALYTTM与2ml的宽pH值SERVALYTTM混合即可。对于超窄pH值SERVALYTTM，我们推荐进行预混合。SERVALYTTM两性电解质载体 (40% W/V)产品名称产品号规格价格(元)SERVALYTTM pH2-442902.0142902.0210ml25ml1500.003080.00SERVALYTTM pH2-1142900.0142900.0210ml25ml1500.003080.00SERVALYTTMpH2-9 Seed-Mix42935.0142935.0242935.0310ml25ml100ml1500.003080.009400.00SERVALYTTM pH3-442922.0142922.0210ml25ml1864.003555.00SERVALYTTM pH3-542903.0142903.0210ml25ml1500.003080.00SERVALYTTM pH3-642944.0142944.0210ml25ml1500.003080.00SERVALYTTM pH3-742945.0142945.0210ml25ml1500.003080.00SERVALYTTMpH3-10 Standard42940.0142940.0210ml25ml1500.003080.00SERVALYTT M pH3-10Iso-Dalt, for 2D 1)42951.0142951.0210ml25ml1864.003555.00SERVALYTTM pH4-542923.0142923.0210ml25ml1864.003555.00SERVALYTTM pH4-642904.0142904.0210ml25ml1500.003080.00SERVALYTTM pH4-742948.0142948.0210ml25ml1500.003080.00SERVALYTTM pH5-642924.0142924.0210ml25ml1864.003555.00SERVALYTTM pH5-742905.0142905.0210ml25ml1500.003080.00SERVALYTTM pH5-7 PGM42936.0142936.0210ml25ml1864.003555.00SERVALYTTM pH5-842949.0142949.0210ml25ml1500.003080.00SERVALYTTM pH5-942950.0142950.0210ml25ml1500.003080.00SERVALYTTM pH6-742925.0142925.0210ml25ml1864.003555.00SERVALYTTM pH6-842906.0142906.0210ml25ml1500.003080.00SERVALYTTM pH 6-942913.0142913.0210ml25ml1500.003080.00SERVALYTTM pH7-942907.0142907.0210ml25ml1500.003080.00SERVALYTTM pH9-1142909.0142909.0210ml25ml1500.003080.00SERVALYTTM pH4-9 T 2)42910.0142910.0242910.0310ml25ml100ml806.001596.005846.00二. 等电聚焦(IEF)电泳预制胶Serva提供一系列的聚丙烯酰胺预制胶用于水平电泳。聚丙烯酰胺胶层结合到支持物上：或者是惰性聚酯膜GEL-FIXTM，该物质活化后能够不可逆的结合到聚丙烯酰胺胶上；或者是聚酯编制物Net FixTM，该物质活化后能够结合聚丙烯酰胺胶上但是胶的两面仍是通透的。如果电泳后需要做blotting的话尤其适合使用Net FixTM，它能够稳定胶但是胶仍旧是通透的，适合转移蛋白。所有预制胶的表面都有保护以免损伤和变干。Serva预制胶是分别包装的，每片胶都包裹在Aluthen袋中以延长保质期。Serva预制胶分为薄型和超薄型。在大多数情况下推荐使用0.33mm胶，在需要高分辨率并且样品量较少时推荐使用0.15mm胶。胶的染色与其它胶相同。1产品性能Properties of SERVA Precast Gels for IEFSERVALYTTMPRECOTESTMSERVALYTTMPreNetsTMBlank PRECOTESTMBlank PreNetsTMPolymer concentration5 % (T)5 % (T)5 % (T)5 % (T)Crosslinker (N,N-methylene-bisacrylamide)3 % (C)3 % (C)3 % (C)3 % (C)SERVALYTMconcentration5 %5 %-Support film thickness (GEL-FIXTM for PAGE)180 um-180 um-Cover film thickness (GEL-FIXTMfor Covers)75 um75 um75 um75 umPolyacrylamide gel layer0.15 mm,0.3 mm0.3 mm0.3 mm0.3 mmRecommended storage4444Shelf life time12 months12 months12 months12 monthsGel formatsLarge standard size245 x 125 mm-245 x 125 mm245 x 125 mmStandard size125 x 125 mm-125 x 125 mm125 x 125 mmMini size( compatible with the PhastSystem1)45 x 50 mm-45 x 50 mm-Delivery/packagingLarge size (245 x 125 mm)5gels/package5gels/package5gels/package5gels/packageStandard size (125 x 125 mm)5gels/package5 gels/package5 gels/package5gels/packageMini size (45 x 50 mm)10gels/package-10 gels/package-2.产品列表PAG LayerSizeCat.No.Quantity priceSERVALYT PRECOTES Wide Range pH 3 - 10150 um125x125 mm42965.035 pcs./包 ￥2607.00150 um245x125 mm42967.025 pcs./包 ￥3444.00300 um125x125 mm42866.025 pcs./包 ￥2598.00300 um245x125 mm42867.025 pcs./包 ￥3444.00300 um45x50 mm42881.0110 pcs/包 ￥1422.00SERVALYT PRECOTES Wide Range pH 3 - 6150 um125x125 mm42974.025 pcs./包 ￥2547.00150 um245x125 mm42919.035 pcs./包 ￥3444.00300 um125x125 mm42874.025 pcs./包 ￥2621.00SERVALYT PRECOTES Wide Range pH 4 - 6150 um125x125 mm42975.025 pcs./包 ￥2547.00150 um245x125 mm42942.035 pcs./包 ￥3444.00300 um125x125 mm42875.025 pcs./包 ￥2621.00SERVALYT PRECOTES Wide Range pH 5 - 7150 um125x125 mm42979.025 pcs./包 ￥2547.00150 um245x125 mm42946.035 pcs./包 ￥3444.00300 um125x125 mm42879.025 pcs./包 ￥2621.00SERVALYT PRECOTES Wide Range pH 6 - 9150 um125x125 mm42978.025 pcs./包 ￥2547.00150 um245x125 mm42954.025 pcs./包 ￥3444.00300 um125x125 mm42878.025 pcs./包 ￥2621.00三等电聚焦(IEF)电泳预制胶试剂盒 l 使用简单，可立即使用l Large format (245 x 125 mm) 用于样本的高通量常规分析l Standard format (125 x 125 mm) 用于筛选分析l 产品进行了优化，适于使用l 有详细的使用说明，结果具有可重复性 1) IEF Starter Kit产品名称产品号规格价格(元)IEF Starter Kit39060.011kit2520.00试剂盒内装：* 3 预制胶 SERVALYTTMPRECOTESTM 125 x 125 mm with PAG layer 300 um* 10 x 2 电极芯electrode wicks * 1 applicator strip* 2 x 10 ml electrode buffer solutions* 5 ml heat exchange liquid (Bayol F)* 100 mg SERVA Blue W tablets,* 100 mg SERVA Violet 17 tablets,* 60 ul IEF marker 3-10 SERVA liquid mix.Determination of pI 3 102) SERVALYTTM PRECOTESTM CSF Kit (用于脑脊髓液的 IEF试剂盒),standard format产品名称产品号规格价格(元)SERVALYTTM PRECOTESTM CSF Kit (用于脑脊髓液的 IEF)42800.011kit4550.00The kit contains: 5 SERVALYT TMPRECOTESTM CSF gels 245 x 125 mm, 300 m Anode and cathode buffer solutions Applicator strips and electrode wicks Optimized protocol for silver stainingSERVALYTTM PRECOTESTM CSF Kit (用于脑脊髓液的 IEF)42801.011kit3297.00The kit contains: 5 SERVALYTTMPRECOTESTMCSF gels 125 x 125 mm, 300 m Anode and cathode buffer solutions Applicator strips and electrode wicks Optimized protocol for silver stainingCerebropinal Fluid3) SERVALYTTMPRECOTESTMHp Kit (血清结合珠蛋白 IEF试剂盒), large format产品名称产品号规格价格(元)SERVALYTTMPRECOTESTMHp Kit (血清结合珠蛋白 IEF)42805.011kit4550.00The kit contains: 5 SERVALYTTMPRECOTESTM Hp gels 245 x 125 mm, 300 m Anode and cathode buffer solutions Applicator pieces and electrode wicks Optimized protocol for silver stainingHaptoglobin Subtyping4) SERVALYTTMPRECOTESTMTf Kit (铁传递蛋白 IEF 试剂盒)产品名称产品号规格价格(元)SERVALYTTMPRECOTESTMTf Kit (铁传递蛋白 IEF 试剂盒)42803.011kit4550.00The kit contains: 5 SERVALYTTMPRECOTESTMTf gels 245 x 125 mm, 300 m Anode and cathode buffer solutions Applicator strips and electrode wicks Instructions for IEF and stainingTransferrin Subtyping5) SERVALYTTMPRECOTESTMPGM Kit (for phosphoglucomutase subtyping),产品名称产品号规格价格(元)SERVALYTTMPRECOTESTMPGM Kit (for phosphoglucomutase subtyping葡萄糖磷酸变位酶)42888.011kit5972.00The kit contains: 5 SERVALYTTMPRECOTESTMPGM gels245 x 125 mm Anode and cathode buffer solutions Applicator strips and electrode wicks Instruction for IEF and detectionSERVALYTTMPRECOTESTMPGM Kit (for phosphoglucomutase subtyping葡萄糖磷酸变位酶)42895.011kit3444.00The kit contains: 5 SERVALYTTMPRECOTESTMPGM gels 125 x 125 mm Anode and cathode buffer solutions Applicator strips and electrode wicks Instructions for IEF and stainingPGM阳极缓冲液Contains 0.56 g L-Histidine Base.For a 0.1 M solution dissolve dry powder in 50 ml distilled water24835.011瓶352.00PGM阳极缓冲液Contains 0.60 g DL-Threonine.For a 0.1 M solution dissolve dry powder in 50 ml distilled water36370.011瓶352.00Sample Application Pieces 10 x 5 mm42880.01200pcs332.00PGM Subtyping四.等电聚焦蛋白Marker由于等电聚焦的高分辨率并能维持自然状态它常被用来鉴定复杂的蛋白混合物，尤其是难以区别的混合物，如一些酶的异构体。在常规分析中，含两性电解质或者固定pH梯度的预制胶可以取得很好的结果。鉴定等电点最常见的方法是同时电泳已知蛋白的混合物。通过比较未知蛋白的位置与已知等电点的marker蛋白的位置能够interpolated非常精确。Serva等电聚焦蛋白Marker的优点：l 可立即用于等电聚焦（ready to use）l 一个标准可用于所有IEF胶（垂直/水平）l 纯化的蛋白组分，不含盐l 可以检测未知蛋白的等电点l 可以监测IEF胶的分离产品名称产品号规格价格(元)IEF Marker 3-10, Liquid Mix39212.01500ml1555.00Protein Test Mixture for pI-Determination, pH 3-1039211.0110mg580.00IEF Marker 3-10, Liquid MixReady-to-use protein marker for isoelectric focusing. Contains 9 proteins pI 3.5 to 10.7 (13 isoforms).Buffer composition: 0.01 % Bromophenol Blue (Na Salt), 0.01 % MethylRed (Na Salt),10 % Glycerol. AmyloglucosidasepI 3.5 Myoglobin horsepI 6.9/7.35Glucose oxidasepI 4.2Lentil lectinpI 7.75/8.0/8.3Trypsin inhibitorpI 4.5Ribonuclease ApI 9.45(beta)-Lactoglobulin pI 5.15/5.3Cytochrome CpI 10.65CarboanhydrasepI 6.0Protein Test Mixture for pI-Determination, pH 3-10pI-Marker proteins for pI determination by isoelectric focusing (IEF). Reconstitute dry powder with 1 ml water (concentration: 10 mg/ml).AmyloglucosidasepI 3.5Myoglobin horsepI 6.9/7.35Glucose oxidasepI 4.2Lentil lectinpI 7.75/8.0/8.3Trypsin inhibitorpI 4.5Ribonuclease ApI 9.45(beta)-LactoglobulinpI 5.15/5.3Cytochrome CpI 10.65CarboanhydrasepI 6.0Schematic representation of marker bands in various pH fractions: Protein (source)PI1) of main bandNo. (band)Cytochrome C (horse, heart)10.79Ribonuclease A (bovine, pancreas)9.58Lectin (Lens culinaris)8.3, 8.0, 7.87 c, m, aMyoglobin (horse, muscle)7.4, 6.9 2)6 c, aCarbonic anhydrase (bovine, erythrocytes)6.05?Lactoglobulin (bovine, milk)5.3, 5.24 c, aTrypsin inhibitor (soybean)4.53Glucose oxidase (Aspergillus niger)4.22Amyloglucosidase (Aspergillus niger)3.51 莆蕿蒀蝿腿蒅葿袁莅莁蒈羃膇芇蒇肆羀薅薆螅膆蒁薆袈罿莇薅羀膄莃薄螀羇艿薃袂节薈薂羄肅蒄薁肇芁莀薀螆肃芆蚀袈艿膂虿羁肂蒀蚈蚀芇蒆蚇袃肀莂蚆羅莅芈蚅肇膈薇蚄螇羁蒃蚄衿膇荿螃羂罿芅螂蚁膅膁螁螄羈薀螀羆芃蒅蝿肈肆莁螈螈芁芇螈袀肄薆螇羃芀蒂袆肅肃莈袅