增生性玻璃体视网膜病变增生膜中α┐平滑肌肌动蛋白的表达.doc

:; :PVR-SMA.14PVRPRM.-SMA14PRMPRM.200PRMC-SMA35/80D-SMA50/60;.:PRMC-SMA12.31.D-SMA23.09C1.88P<0.01.-SMAPVR. Keywords:vitreoretinopathyproliferative;actins;immuno-histochemistry Abstract:AIMToinvestigatetheexpressionofalpha-smoothmuscleactin-SMAwithintheperiretinalmem-branePRMofPVR.METHODSFourteenspecimensofPRMwereobtainedduringvitrectomiesofpatientssufferedfromPVRofdifferentgrades.-SMAexpressioninPRMweredetectedbyimmunohistochemicalstainingandquanti-tativeanalysisperformedbyimageprocessofimmunofluorescence.RESULTS-SMAexpressionwasdetectedinall14PRMs.Thedistributionsof-SMAwerepresentinthecytoplasmaandaccordancewiththedirectionofstressfibers.-SMAexpressionwasstrongerinPVR/DthaninPVR/C.CONCLUSIONExpressionof-SMAwasacom-monfeatureofPRMandtheexpressioncorrelatedwithseverityofPVRandtheprocessofmembranetraction. 0 proliferativevitreo-retinopathyPVRPRM12.RPEPVRRPE3.-SMA 4.McGillem5:PVR-SMA-SMAPVR.PVR-SMA. 1 1.1 1998-07/1999-11PVR144632mo2a.PVR1983C3-D3.59.40g・L-130min4.5m.-SMA1200;DABDAKOAvidinTexasRedMolecularProbes.1.2 1.2.1 7.5mL・L-1H2O2-PBS3730min.3730min.PBS3IgG3730minABC3730min.TexasRed3730min.;RPE. 1.2.2 DAB:OLMPUSBX50200. TexasRedBIO-REDMR-1024:. 2 :DAB;TexasRed595nm. DAB14PRM-SMA.6PVR/C3PRM-SMA35/80:;Fig1;8DPRM-SMA50/60Fig2. DAB.CFig3;DFig4. 3 14PVR-SMA-SMACDPRMC;DPRM.PVR-SMAPRM. -SMA.6.7.8. RPEPVR9RPE1011.Lewis12PRMRPE.13. RPEPVR14.RPE-SMA5.-SMAPVR15. CPRM-SMA-SMA;DPRM-SMA-SMADC1.88.PRM.RPERPECPRM-SMA;DPRMRPE-SMA-SMARPEPRM.PVRRPERPE. : 1WellerMWiedemannPHeimannK.Proliferativevitreo-retinopathyisitanythingmorethanwoundhealingatthewrongplaceJ.IntOphthalmol1990;142:105-117. 2HuiYNSorgenteNRyanSJ.PosteriorvitreousseparationandretinaldetachmentinducedbymacrophagesJ.GraefesArchClinExpOphthalmol1987;1073:1147-1151. 3VinoresSACampochiaroPAMcgeheeR.UltrastructuralandimmunocytochemicalchangesinretinalpigmentepitheliumretinalglialandfibroblastsinvitreouscultureJInvestOph-thalmolVisSci1990;3112:2529-2545. 4StodtlerMMietzHWiedemannP.ImmunohistochemistryofanteriorproliferativevitreoretinopathyJ.IntOphthalmol1994-95;186:323-328. 5McGillemGSDacheuxRF.Rabbitretinalmullercellsundergoantigenicchangesinresponsetoexperimentallyinducedprolif-erativevitreoretinopathyJ.ExpEyeRes1999;685:617-627. 6GrisantiSGuidryC.Transdifferentiationofretinalpigmentepi-thelialcellsfromepithelialtomesenchymalphentypeJ.In-vestOphthalmolVisSci1995;362:391-405. 7SchurchWSeemayerTAGabbianiG.Myofibroblastinstern-bergssedHistologyforpathologistsM.NewYork:RavenPress1992;109-114. 8NedeleeMGhaharyAScottPG.Controlofwoundcontrac-tion.BasicandclinicalfeaturesJ.HandClin2000;1612:289-302. 9TotiPGrecoGCatellaAM.Morphologicalandpathogeneticaspectsofproliferativevitreo-retinopathy.AhistologicalandimmunohistochemicalstudyJ.DocOphthalmol1994;882:105-112. 10VinoresSACampochiaroPAMcgeheeR.Simultaneousex-pressionofkeratinandglialfibrillaryacidicproteinbythesamecellsinepithelialmembranesJ.InvestOphthalmolVisSci1992;3312:3361-3366. 11CampochiaroPA.PathogenicmechanismsinproliferativeviteroretinopathyJ.ArchOphthalmol1997;1152:237-241. 12LewisGPMatsumotoBFiaherSK.Changesintheorganiza-tionandexpressionofcytoskeletalproteinsduringretinalde-generationinducedbyretinaldetachmentJ.InvestOphthal-molVisSci1995;3612:2404-2416. 13GabbianiMajnoG.Dupuytrenscontracture:Fibroblastcon-traction?anultrastructuralstudyJ.AmJPathol1972;661:131-146. 14CampochiaroPA.Pathogenicmechanismsinproliferativeviteroret