个体化医疗的现状与未来.ppt

个体化医疗的现状与未来四.蛋白质微阵列技术,.,TheMajorTechniquesinCurrentProteomics,Two-dimensionalelectrophoresisIEFstripseparationSDS-PAGEgelseparationMassSpectrometryProteinsequencingPeptidemappingOthersYeasttwohybridassayProteinmicroarray,传统蛋白质组研究方法,二维凝胶电泳、质谱技术-需要复杂的设备、样品的需求量大，可重复性低，-在低丰度蛋白检测存在缺陷，且对蛋白水解为多肽不易控制，不利于可靠的数据分析-不能用来作为蛋白质生物标记物的验证,AndersonLetal.JPhsiol563(1):23-60,2005,ProteinMicroarrays,Theoreticalconsiderationsmacrospot-microspot,RogerEkins:ambientanalytetheory,AdvantagesofMicroarray,HighsensitivityMuchhigherthroughputMoreflexibilityLesssampleconsumptionQuantitationDirecttargetdetection,Smallerisbetter,一种蛋白质的所有变异体一个蛋白质家族的所有成员一条信号通路中的所有蛋白质一种组织、器官或有机体的所有蛋白质,一个芯片上可以容纳,DifferentKindsofProteinArrays,AntibodyArrayAntigenArrayLigandArray,Detectionby:SELDIMS,fluorescence,SPR,electrochemical,radioactivity,microcantelever,抗体微阵列(antibodymicroarray),将抗体作为捕获分子固定于载体表面，通过抗原抗体反应，实现对样品中特定蛋白质的平行性、高通量检测。基于既往对疾病的理解，对疾病相关蛋白质进行高通量的靶向分析。,HaabBB.AACR97thAnnualMeetingApril1-5,2006,Applications,DiscoverproteinexpressionpatternsformolecularclassificationofdiseasesScreenmoleculartargetsfordrugdevelopmentDistinguishmolecularmechanismsofdrugactionDefinebiomarkersfordiseasemanagementMonitorcytokineprofilesofbiologicalsamples,Keystepsinantibodymicroarraydesign,thecapturemolecules:chemicallyrobust,highaffinityImmobolization:strongattachment,retentionoffunctionthedetectionsystems:sensitive,signalamplified,easyobserved,AttachmentMethods:,DiffusionAbsorptionnitrocelluloseCovalentCrosslinkingReactivesurfacesAffinityAttachmentAffinitytags,CapturemoleculesSourceTechnique,mAbMouseHybridomascFv/FabdiabodiesAntibodylibrariesPhagedisplay,invitroAffibodiesMicroorganismHeterologousexpressionAptamersLibrarySELEX/mRNAdisplay,(DNA/RNA/peptide)ReceptorligandsSyntheticCombinatorialchemistryAffinitybindingagentsRecombinantInvitroevolution,Capturemolecules,Detection,国内生物芯片市场,上海博星,上海生物芯片,北京博奥,陕西超英生物科技,西安联尔2002年，国家药品和食品监督管理局批准了5种生物芯片，除江南生物的地中海贫血基因检测芯片外，