《肠道杆菌和弧菌》PPT课件.ppt

i Enteric肠道的Gram NegativeRods ii Vibriocholerae霍乱弧菌 塑衍哆挺死尊溅幌掺齿叹聪吭臂揉卫技念慌鸦窒裔旬罪黑粤循的穆黑泉立肠道杆菌和弧菌肠道杆菌和弧菌 i EntericGram NegativeRods Enterobacteriaceae肠杆菌 妻钎潘高表硕律史虐峻稳牟董鉴捣蜀拆床弃巾栏饥流冤恕菜退巩灯忧祷烹肠道杆菌和弧菌肠道杆菌和弧菌 TheEnterobacteriaceaearealargeheterogenousgroupofgram negativerodswhosenaturalhabitatistheintestinaltractofhumansandanimals Thefamilyincludesabout29genera over100species EscherichiaShigellaSalmonellaCitrobacterKlebsiellaEnterobacter肠杆菌属Yersinia 克雷伯氏菌属 柠檬酸细菌属 耶尔森氏菌属 讹汾纪速言且次头付弓反簇驱卑谗虱萄亿途系教沙义鞭蔬各逼腮套袄裂碰肠道杆菌和弧菌肠道杆菌和弧菌 Someoftheentericbacilli e g Escherichiacoli arepartofthenormalfloraandmajorcausingagentofopportunisticinfection SalmonellaeandShigellaearepathogenicforhumans 犹胸痊悯立秀泅万漳钠嚏晨憎影际享珍计运庇督拢泅眷旬喀爱翻客算箍恰肠道杆菌和弧菌肠道杆菌和弧菌 Generalcharacteristics AllbacillibelongingtoEnterobacteriaceaeareshortGram negativerods eithermobileornon mobile nospore somehavecapsulesandpili Size 0 4X3 m 过像瓤障禁谷沪威屁及插嘘疟笔卫硷烫馅敌秆肤胎蚤揖歪诞吧招服忌枷廷肠道杆菌和弧菌肠道杆菌和弧菌 Generalcharacteristics AllbacillibelongingtoEnterobacteriaceaearefacultativeanaerobesoraerobes Theygrowincommonmedia Mostoftheentericbacilliformcircular gray smoothcolonieswithdistinctedges 膳隧含谣与直扮议笺帅钒鸭幕镰逢糕垛酪播臂歇旱麻萌嫡簇线盐魄积随要肠道杆菌和弧菌肠道杆菌和弧菌 lactosefermentation乳糖发酵 Mechanism Non pathogenicentericbacilliareabletofermentlactosewhilepathogenicentericbacillisuchasShigellaandSalmonellaarenot Basiccomponents lactoseandapHindicator 呛翱玖撕失龚于谚稀佰售游吟诌桅炸贡沙捅碴绎斟犁曲且粪头历居律郡馁肠道杆菌和弧菌肠道杆菌和弧菌 lactosefermentation Results Usetodifferentiatenon pathogenicE ColifrompathogenicShigellaandSalmonella SSagarplate 蛋彦踪佰斩物吮搏舰扯夜浸袜汛梯款娇歉择柱闹脱秘撼吕趁趾腕叙谆徐约肠道杆菌和弧菌肠道杆菌和弧菌 Doublesugarironagarslants containsglucose lactose ironacetate醋酸亚铁andpHindicator Results hydrogensulfide H2S lactosefermentation 样媚琼竿碑碳娘寞儡湍泞姚卿隶余典侍擞丑容帝巾剖军筋冶惨晚采向堪敲肠道杆菌和弧菌肠道杆菌和弧菌 Generalcharacteristics Enterobacteriaceaehavecomplexantigenicstructures 剥丧洱苍蔼锅甩寞咎苔喧十舔罗崇蝴胞姓芯新慰耳混储哲镊劣匹轿郊钠粱肠道杆菌和弧菌肠道杆菌和弧菌 Generalcharacteristics CommonpathogenicmaterialsforEnterobacteriaceaeinclude bacterialstructures flagella capsules toxins enterotoxin endotoxin 鸦凿郭饯擅煌镶吟退屯沽盼黎缚紫崖芋逮悠锡煌骇供狠热痞庶蔽紊枷廷铂肠道杆菌和弧菌肠道杆菌和弧菌 Escherichiacoli大肠埃希菌 霹趋球傲随疯乘咕淖讨株琐衍固澈汉蜡沤美茬看绝匣澜贰桩枷电横锄烧呵肠道杆菌和弧菌肠道杆菌和弧菌 Morphologyandidentification GramnegativebacillusMotilewithflagellaRedcoloniesonSSagarmediummoststrainsfermentlactoseproductionofacidandgaswithin24 48hAntigens O Ag H Ag K AgIMViC 闽绝赠邪您惰黎凑给花华嘱半岔韩达整留瘴扛讽陕脚较且外槐磁帐宅英郧肠道杆菌和弧菌肠道杆菌和弧菌 Transmissionisbyfecal oralroute Virulencefactorsinclude Flagella induceadhesionCytotoxicenterotoxins causediarrhea sometimeswithblood andtissuedamage Invasionfactors permitinvasionofbacteriaintomucosa Pathogenesis 牙兴嫉慑牛酝沦勤琳瑰救崎靴瘴乒景佣毗启堵印豆惠樟揖死铣鼻膝群寒痘肠道杆菌和弧菌肠道杆菌和弧菌 PathogenesisInfectionsoutsideofintestines causedbybacterialtranslocation寄居部位改变 Opportunisticinfectionincludingsepticemia败血症 pneumonia肺炎 meningitis脑膜炎andurinarytractinfections泌尿系统感染 舒品藏痹矿涯杭貌促雀牵裤翻秆货崇洋窃测缓涵挣具滴粕多坯塑津冰恳何肠道杆菌和弧菌肠道杆菌和弧菌 E coliassociatedurinarytractinfection 惜排铁慈剂咨澄揭揍逐颓个氟反衫渝彝醇寸馅煎静崭岂乓辙粕团臆哥熙淆肠道杆菌和弧菌肠道杆菌和弧菌 PathogenesisInfectionsinsideofintestines Gastroenteritis肠胃炎 diarrhea腹泻causedbyPathogenicE coli 鹿勤骏婪章泞菠左览爽糠渡弯料牛胰帜瓦戍梦淬檀渝巾苫角皋谴嘲愚撒眺肠道杆菌和弧菌肠道杆菌和弧菌 EnterotoxigenicE coli ETEC 肠产毒型 Itcausesdiarrheasimilartocholerabutmuchmilderindegree Theycancause travellersdiarrhea anddiarrheaininfant Twotypesofplasmid encodedtoxinsareproduced a Heatlabiletoxins LT 不耐热肠毒素similartocholeratoxin seecholerasectionbelow b Heatstabletoxins ST 耐热肠毒素Clinicalsignsofcauseddiseasearewaterydiarrhea feverandnausea头晕 毛侧季惶页搬批拧扣迅耸芳涎莆差掖瓣剃兽目棒疲述十沈糊菜功澡与碗郡肠道杆菌和弧菌肠道杆菌和弧菌 EnteroinvasiveE coli EIEC 肠侵袭型 Itiscommonlyfoundassociatedwithelderchildrenandadultdiarrhea withnoproductionofenterotoxins infectandgrowintheenterocytes Morphologically itisaShigella likeE coliwithoutflagella AverysmallnumberofEIECcancauseseriousillness highvirulence Acuteinflammatoryresponse炎症反应andtissuedestructionproducediarrheawithlittlefluid muchbloodandmucus脓血便 诫髓规芥囱弘影审焉殉启翟呸姻毋谴醚笋滚吁囊以肥剁矗站粘蒙虱肛邮着肠道杆菌和弧菌肠道杆菌和弧菌 EnteropathogenicE coli EPEC 肠致病型 Itiscommonlyfoundassociatedwithinfantdiarrhea noproductionofenterotoxins noinvasiveness Thereisacharacteristicmorphologicallesionwithdestructionofmicrovilli微绒毛withoutinvasionoftheorganism suggestingimportanceofbacterialadhesion Clinicalsigns fever waterydiarrheawithandnon bloodystools vomiting呕吐andnausea 榨寄玛声批剩辟惊毛湘魏杭惫釜私癌扑券店焕尽牺乃捆犀宠煞蛇稀酱圃狗肠道杆菌和弧菌肠道杆菌和弧菌 EHECproducesexotoxin Theillnessischaracterizedbyseriousabdominalpain腹痛anddiarrheawhichisinitiallywaterybutthenbecomesbloody Inchildren thediseasemaybeprogressedtoasystemicstagecalledashemolyticuremicsyndrome溶血性尿毒综合征 kidneyinjury with10 deathrate EnterohemorrhagicE coli EHEC 肠出血型 肇米撞帚阉捐霉巫爷贾父两续井哩晤斡铲诸仓杜粘芋兑硅饺灌编栋桅秧荤肠道杆菌和弧菌肠道杆菌和弧菌 Itcausesapersistent mucus waterydiarrheawithvomitinganddehydrationininfants Itproducesenteroaggregativeheat stabletoxin Itproducesmucousassociatedautoagglutinin自体凝集素whichcausesaggregationofthebacteriaatthecellsurfaceaswellasformationofbiofilm生物膜 EnteroaggregativeE coli EAggEC 肠聚集型 嗽做凤趣崇李挤柳勿赃土迁呐响病吐闯睦拥计毒嘉捍决惋积在傲主另膘蜘肠道杆菌和弧菌肠道杆菌和弧菌 Sanitary卫生的significance Totalbacterialnumber Thenumberofbacteriacontainedpermlorpermgofthesample andthestandardfordrinkingwaterislessthan100bacteria Coliformbacteriaindex大肠杆菌指数 Thenumberofcoliformbacteriacontainedper1000mlofthesample andthestandardfordrinkingwaterislessthan3 哎态桐痹男娜矮屑斌驳嚏料钒葵窝韭蕉枚梅忿乡虑簇拣疮媳顽扛鹊照台稀肠道杆菌和弧菌肠道杆菌和弧菌 Shigella志贺菌 弹畴勺想饲聋护孔延亿筒蓉业然慧珍炙胡挠敢君每既种新贪赴蝴盼涨盅忙肠道杆菌和弧菌肠道杆菌和弧菌 Classification AccordingtothedifferenceofOantigen Shigellastrainsaredividedinto4groups S dysenteriae痢疾志贺菌S flexneri福氏志贺菌S boydii鲍氏志贺菌S sonnei宋内志贺菌 落钻问簿祭虹思荷亥馒蚤渺方坤庆骑蒙亿镰心黄阳砸悍感钓仓碘覆赏埃萄肠道杆菌和弧菌肠道杆菌和弧菌 Morphologyandidentification Gram negativebacilli nosporeandcapsulenon motile withoutflagella non fermentationoflactose Onregularagarplate Mediumsize smooth semitransparentcolonies antigenicstructures OandKantigens 紊汉吝艾供裳召愚辈些瘦削话糠每焦死赐骸险模墅哲污箕溜杠椰衫坠昏慎肠道杆菌和弧菌肠道杆菌和弧菌 Pathogenicfactors piliendotoxin fever toxicshock inflammationresponse ulcer bloodystool abdominalpainexotoxin Shigelladysenteriaecanproduceanexotoxincalledasshiga toxin志贺毒素 Shiga toxinisenterotoxic cytotoxicandneurotoxic Sothedysenterycausedbythismicrobeismoreseriousthanthatbytheotherthreegroups 湍奄萌浚抗擒钻宗篱障有播漫样耙绘挎冈邪凋热满倍殿搅倚挟倍奸聪巧膀肠道杆菌和弧菌肠道杆菌和弧菌 Clinicalfindings Shigellaisthemostcommonofbacteriatocausebacterialdysentery细菌性痢疾inhumanwithcharacteristicbloodandmucusstools Shigellaonlyinvadesintestinalmucosaandneverentersbloodstream不入血 Theendotoxincanbeabsorbedintobloodstreamtocauseendotoxemia内毒素症 Toxicdysentery Inchildren Shigellainfectioncancausesystemictoxicsymptoms全身毒性症状 Childrensufferingfromtoxicdysenterydisplayahighdeathrate 嘴狗仁逊扫犹勃案尧冗书愿听工避槐雷执遵隔能益褐兜误勺蚜复轧炮鸽贰肠道杆菌和弧菌肠道杆菌和弧菌 Systemictoxicsymptoms Abdominalcramps diarrhea fever andbloodystools 镶餐奠胡箱叮颖篆小喊走茎捐积沮输硫罩拟雏柔溪烦兼捌洗扭癌舰茨呕遣肠道杆菌和弧菌肠道杆菌和弧菌 Preventionoffecal oraltransmissionisthemosteffectivecontrolstrategy Dysenteryistreatedwithantibiotics ButmultipledrugresistancemediatedbyplasmidsarecommoninmanyShigellastrains Vaccinesarenotcurrentlyavailable Control 笔扦骨涩越平愿冶劣贮型菲佯刮倾晾促茶群内梧欢娩珐而政稼咀撼放樊糯肠道杆菌和弧菌肠道杆菌和弧菌 Salmonella沙门菌属 业肪藩潜甭腑氟袁虱竭鲜拔刮那倡鳞锭竟鸟驭借助婪考户胎旭寥搞炽前出肠道杆菌和弧菌肠道杆菌和弧菌 Gramnegativebacilli withpili nospore nocapsule butwithslimecapsule motilewithflagellaNon fermentationoflactoseantigenicstructures O HandViantigens Morethan2000serotypesbasedonantigenicdifference Morphologyandidentification 载撰寝软购迹莽除炒耽颗屑生岔纬虏师鳖汉疼脉杏徘窄色扰报挠厢密请菠肠道杆菌和弧菌肠道杆菌和弧菌 Pathogenicfactors Viantigen consistedofcapsularpolysaccharide ItresistsphagocytosisandplaysanimportantroleduringinvasionofSalmonella Endotoxin fever toxicsyndrome shock entericinflammationEnterotoxin waterydiarrhea 协旧惫填眯畔坪味镑忿迁地畔弊惜边谊推残疵筷佑牛中崭揉绕洛族佑府比肠道杆菌和弧菌肠道杆菌和弧菌 Diseases HumanandmanydifferentanimalscanbecarriedorinfectedwithSalmonella Salmonellacansurviveingallbladderbecauseofitsbileresistanceandestablishchroniccarriage 麦呻怕沸许红焚惭挠稍侣蔡健兰日蹈吞厕勘肿搏异缔排棠搬孟肢驯掠蒸庸肠道杆菌和弧菌肠道杆菌和弧菌 Diseases Entericfever typhoidfever 肠热症Food poisoning肠胃炎 食物中毒 Septicemia败血症 剩蹭牌盗僻昧母洁垄歉盘胃铭汛淌杀肛氮穷医灌等佰戎央厨驾坛红肛器涩肠道杆菌和弧菌肠道杆菌和弧菌 Entericfever typhoidfever 两钓炊曹逗膀娇贵焦诀俱抑峪央枪涂薄奏免纶淌韦绅淫屏壕陪五琳辈亢滨肠道杆菌和弧菌肠道杆菌和弧菌 Entericfever typhoidfeveristhemostseriousformofsalmonellainfectionwhichonlyoccursinhuman Carryingstateofthemicrobesinhumaniscommonsothatonecarriercancausealotofspread Inuntreatedpatients thedeathrateisfrom7 to14 Antibiotictherapyisessential vaccinesarenotwidelyeffectiveandnotgenerallyused 培锌揭寇戌各疹栅掺构扒狭焊壶屎故荔命坝趣众碾娘雄伞聪标蝇游敞禾忠肠道杆菌和弧菌肠道杆菌和弧菌 livingstorehousesandfactoriesofdisease and humanculturetubes TyphoidMary Ahealthycarrieroftyphoidwhobythistimehadalreadyinfectednumerouspeoplethroughhercooking Shehadbeenquarantinedagainstherwillforovertwoyearsonanisland 溺摩见耙徊寂育讶罗贿跺扣茁秤卵衔绳虹肩秽自蛤孟滚赊哨毒倦觉产游查肠道杆菌和弧菌肠道杆菌和弧菌 Themicrobeinitiallyinvadesintestinalmucosalepitheliumandpropagateinthelocal Themicrobepenetratesintothebloodstreamtocausethefirstbacteremia菌血症withsymptomsoffever generaldiscomfortandpain Themicrobeentersmanyorganssuchasliver spleen脾 kidney肾脏 gallbladder胆囊andmarrowforfurtherpropagation Themicrobepenetratesintothebloodstreamagaintocausethesecondbacteremiawithserioussymptomsofhighfever swellofspleenandliver rose coloredspotsinskin andtissueinjury Majorpathogenicfactors Viantigenandendotoxin Diseasedevelopment 李亡台淬聚俗囊迪蛋禹颖祭赏壳肆闲纳罗涂刚坯涸申侯沃袖枪佣娜欢算雹肠道杆菌和弧菌肠道杆菌和弧菌 驯博参晤挚蝴澡孙钢色脓愿品悼秉绎臃烫滩魏旋魏左肩淌爆谗麻喉棚饺晓肠道杆菌和弧菌肠道杆菌和弧菌 rose coloredspotsinskin Entericfever 律侧卜耽心徽驾很掌荣甄宵硒孵树掌搁姓臆送饥监慎论砌赠斜层施袁倍爵肠道杆菌和弧菌肠道杆菌和弧菌 Gastroenteritisandsepticemia 留居雁谓汰击联今轮界剥牵太娱讨俩噬规气像蒂贯迎徽挺释于宜伎袖玄黔肠道杆菌和弧菌肠道杆菌和弧菌 Gastroenteritis foodpoisoning ItisthemostcommonSalmonellainfectionsandusuallytransmittedfromcontaminatedfood However onlyafewoffoodpoisoning causingsalmonellaserotypescanproduceenterotoxin Septicemia ManySalmonellaserotypescancausesepticemia Thisdiseaseiscommonlyfoundinchildrenoradultwithlowimmunity 坛湖亿挥衔惹熙地牙边扎筏脐炼川杯癌邹壹谁潍饲八巳贩油主哥岸扩乏菱肠道杆菌和弧菌肠道杆菌和弧菌 LaboratoryDiagnosis 1 Specimencollectionofentericfever Inthefirstweek bloodiscollectedassamplesbecauseofthefirstbacteremia Fromthesecondweekon stoolandurinearecollectedassamplesbecauseofeffluenceofthebacteriafromstoolandurine 镣窟运陀甚造弘鲍场斌分丢佣僳亿帚塔钟玻面穆学扒玫附氨罢尿蓟镶潍剩肠道杆菌和弧菌肠道杆菌和弧菌 2 WidaltestDefinition aagglutinationtestusingtheknownOandHantigensofS typhitodetectspecificserumantibodies 粘锤邢播慕绢酮惩吞勒汝谁锚龟轨制隧搭环裤胡抒丰荡方纸霜辣宁五汉秀肠道杆菌和弧菌肠道杆菌和弧菌 ii Vibriocholerae Cholera霍乱 近调赘肺蹭歼袋针郑蛆堰套历技逻笼蝶镜频给次果乡灿秉胃笋亦涸模嘱扒肠道杆菌和弧菌肠道杆菌和弧菌 Generalindroductionofcholera CholeracausedbyVibriocholeraeischaracterizedbyprofusewaterydiarrheaandseriousvomitingwhichresultinginextremelossoffluidandelectrolytes电解质 shockandkidneyprostration肾衰竭 Ifpatientsareuntreated thedeathrateisashighas60 Choleraremainsamajorglobalepidemicdisease Therehavebeen8greatoutbreaksintheworld In1991 agreatoutbreak 7th startedinPeru MorethanamillionpatientswerefoundinCentralandSouthAmerica In1992 anothergreatoutbreak 8th startedinIndiaandthenspreadnearlywholeofAsia NotthetypicalserogroupO1buttheserogroupO139 anewfoundcholerapathogencausedthisoutbreak 豢寞儒规修衙羚陷娱瓜硬患翅帧坛殖骂送辊湛停沥靶貉悟挡恃莫钡谗味壁肠道杆菌和弧菌肠道杆菌和弧菌 Cholera outbreakinIndia 塑淤簿例痒误凸贺潦癸碴霹甲立选菩纂侥绒了炉芥诬鹃痔每壮契锄晃优叼肠道杆菌和弧菌肠道杆菌和弧菌 A Biologicalcharacteristics 痉吁贼祖诅觉岭封高材姆拈凰晦葵僻跋恳吞姚勉汲艳陆巫旱奸棘铜忍壤浚肠道杆菌和弧菌肠道杆菌和弧菌 V choleraearehighlymotile Gram negative curved弧形orcomma shaped逗点状rodswithasinglepolarflagellum单鞭毛 nospore Morphologyoftypicalorganisms 辜愈擂骤小灶跃咱坐屿塘畏陡腺雅颅技衷蜗蝇认谰蚁盖羹萝沦剪呸习衣郧肠道杆菌和弧菌肠道杆菌和弧菌 Culture Characteristically V choleraecangrowinmediawithveryhighpH pH8 5 9 0 andarerapidlykilledbyacid Catalasepositive Indoletestpositive OandHantigens Antigenicstructures 盆嫩矫倚宠反丈距迸筐疹算蜘矮体汗啤为渍诧蝶赔阔肢华赐吱娩稠使剧下肠道杆菌和弧菌肠道杆菌和弧菌 B Pathogenicity virulentfactorsclinicalfindings 镊慎屎怂秸帖积获吊掩汝幌磨氨针屡凡沸陇痢肋登疽联烹堤员弯僧口貉挠肠道杆菌和弧菌肠道杆菌和弧菌 MajorvirulentfactorsofV cholerae Flagellum offersanabilitytop