3)工艺优化.pdf

Filtration Separation Solution SM Life SciencesLife Sciences Pall Corporation 2002 Running an Optimization Trial 优化切向流工艺参数优化切向流工艺参数 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Presentation Agenda 摘要摘要 Planning切向流工艺方案设计切向流工艺方案设计 Optimization优化切向流过滤参数优化切向流过滤参数 Concentration浓缩工艺摸索浓缩工艺摸索 Diafiltration洗滤工艺摸索洗滤工艺摸索 Product Recovery 产品回收工艺摸索产品回收工艺摸索 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Planning 设计工艺方案设计工艺方案 Process objectives工艺目标工艺目标 Consider large scale process and process requirements e g SIP 考虑大生产工艺要求考虑大生产工艺要求 Select correct platform选择合适的平台选择合适的平台 Select membrane rating MWCO选择截留精度选择截留精度 Select correct channel format选择适合的通道类型选择适合的通道类型 Determine area to be used for trial考虑实验场所考虑实验场所 Acquire appropriate volume of sample准备合适的样品准备合适的样品 Sample should be truly representative of product 和实际工艺物料一致和实际工艺物料一致 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Planning 设计工艺方案设计工艺方案 Trial considerations实验设计实验设计 Establish volume to be used for trial料液体积料液体积 Membrane area to volume ratio面积与体积比率面积与体积比率 Approximate to full scale process接近实际工艺接近实际工艺 Concentration factor required浓缩因子浓缩因子 Hold up volume and dilution死体积和稀释死体积和稀释 Diafiltration requirements 是否需要洗滤是否需要洗滤 Product stability e g temp 产品稳定性产品稳定性 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Planning 设计工艺方案设计工艺方案 Equipment requirements设备要求设备要求 Pump 泵泵 Hold up Min working volumes 最小工作体积 最小工作体积 Know it and minimize it 越小越好 越小越好 Correct range of instruments设备正确安放设备正确安放 Calibrated instrumentation校验计量设备校验计量设备 Pressure gauges 压力表压力表 Thermometer 温度计温度计 Temperature control 是否需要控制温度是否需要控制温度 Flowmeter 是否需要流量计是否需要流量计 Use spreadsheet if possible 最好使用电子表格记录数据最好使用电子表格记录数据 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Recirculation TFF System Configuration Optimization 系统配置优化系统配置优化 PUMP RETENTATE FEED FEED VESSEL PERMEATE Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Flux Vs Transmembrane Pressure 料液过滤通量与过膜压力关系料液过滤通量与过膜压力关系 PROCESS FLUID物料物料 MEMBRANE CONTROLLED REGION 膜控制区膜控制区 GEL LAYER CONTROLLED REGION 凝胶层控制区凝胶层控制区 Transmembrane Pressure过膜压力过膜压力 Filtrate Flux Rate通量通量 CONSTANT CF OPTIMALOPTIMAL P 恒定切向流速恒定切向流速 最佳过膜压力最佳过膜压力最佳过膜压力最佳过膜压力 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 TFF System Configuration Optimization工艺优化演示工艺优化演示 PUMP RETENTATE FEED FEED VESSEL PERMEATE PUMP RETENTATE FEED FEED VESSEL PERMEATE Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 PUMP RETENTATE FEED FEED VESSEL PERMEATE TFF System Configuration Optimization工艺优化演示工艺优化演示 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 PUMP RETENTATE FEED FEED VESSEL PERMEATE TFF System Configuration Optimization工艺优化演示工艺优化演示 Add the permeate collected back into the feed vessel 将滤出样品倒回进料罐将滤出样品倒回进料罐 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Optimization工艺优化工艺优化 Flux Vs TMP Plot通量 过膜压力曲线通量 过膜压力曲线 Flux Vs TMP 0 00 10 00 20 00 30 00 40 00 50 00 0 000 501 001 502 00 Transmembrane Pressure Permeate Flux Rate LMH 0 00 0 25 0 50 0 75 1 00 1 25 Process FluxDifferential Pressure Crossflow 0 7L min 1 00 bar P Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 PUMP RETENTATE FEED FEED VESSEL PERMEATE TFF System Configuration Optimization工艺优化演示工艺优化演示 PUMP RETENTATE FEED FEED VESSEL PERMEATE PUMP RETENTATE FEED FEED VESSEL PERMEATE Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Optimization工艺优化工艺优化 进料压力回流压力滤出压力过膜压力压差回流速度滤出速度滤过通量进料压力回流压力滤出压力过膜压力压差回流速度滤出速度滤过通量 0 00 00 00 00 00 00 00 0 0 700 001 000 00 51 03019 5 0 251 251 00 00 700 754026 0 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Optimization工艺优化工艺优化 Flux Vs TMP 0 00 10 00 20 00 30 00 40 00 50 00 0 000 501 001 502 00 Transmembrane Pressure Permeate Flux Rate LMH 0 00 0 25 0 50 0 75 1 00 1 25 Process FluxDifferential Pressure Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Optimization工艺优化工艺优化 进料压力回流压力滤出压力过膜压力压差回流速度滤出速度滤过通量进料压力回流压力滤出压力过膜压力压差回流速度滤出速度滤过通量 0 00 00 00 00 00 00 00 0 0 700 001 000 00 51 03019 5 0 251 251 00 00 700 754026 0 0 501 501 00 00 701 05032 5 0 751 751 00 00 701 255837 7 1 002 001 00 00 691 506340 1 1 252 251 00 00 681 756542 3 Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Optimization工艺优化工艺优化 Flux Vs TMP 0 00 10 00 20 00 30 00 40 00 50 00 0 000 501 001 502 00 Transmembrane Pressure Permeate Flux Rate LMH 0 00 0 25 0 50 0 75 1 00 1 25 Process FluxDifferential Pressure Flux Vs TMP 0 00 10 00 20 00 30 00 40 00 50 00 0 000 501 001 502 00 Transmembrane Pressure Permeate Flux Rate LMH 0 00 0 25 0 50 0 75 1 00 1 25 Process FluxDifferential Pressure Flux Vs TMP 0 00 10 00 20 00 30 00 40 00 50 00 0 000 501 001 502 00 Transmembrane Pressure Permeate Flux Rate LMH 0 00 0 25 0 50 0 75 1 00 1 25 Process FluxDifferential Pressure Flux Vs TMP 0 00 10 00 20 00 30 00 40 00 50 00 0 000 501 001 502 00 Transmembrane Pressure Permeate Flux Rate LMH 0 00 0 25 0 50 0 75 1 00 1 25 Process FluxDifferential Pressure Pall Corporation 2005 Filtration Separation Solution Filtration Separation Solution SMSM Pall Corporation 2003 Optimization工艺优化工艺优化 Flux Vs TMP 0 00 10 00 20 00 30 00 40 00 50 00 0 000 501