免疫学试题A卷及答案.doc

The final examination of medical immunologyfor seven year students of Grade 2003(2005/12/6)Paper A 题号一二三四五总分统分人得分得分 评卷人I. Explain the following items (2 points for each, 18 points)1. Lymphocyte recirculation 扣分2. CDR 扣分3. cytokine 扣分4. MHC restriction 扣分5. PRR 扣分6. Immune tolerance 扣分7. ITAM 扣分8.APC 扣分9. TI-Ag 扣分II. Choose all correct answers (1 point for each,15 points)得分 评卷人 No.12345678AnswerNo.910111213Answer1. Which of the following statement(s) about the spleen is(are) true: A. It filters antigens out of the blood.B. Lymphatic vessels draining the tissue spaces enter the spleenC. It contains germinal centersD. It functions to remove old and defective red blood cells 2.Which kinds of Ig expressed mainly on the surface of mature B cells? A. mIgG B. mIg A C. mIgD D.mIgM3.Which can function as C3 convertase in activation of complement system?A.C4b2b B.C4b2b3b C.C3bBb D.C3bnBb4.About the characteristics of cytokines, which of the following(s) is (are) correct?A. One cytokine can act on different cells and play multiple biological effects B. The cytokine produced by one cell only acts on neighbor cells C. Combined with corresponding receptor to play roleD. One cytokine can inhibit or enhance effects of other cytokine5.Which of the following molecules can stimulate T cells activation?A. LPS B. PWM C. Con A D. PHA 6.What are the functions of B cells?A. produce AbB. secrete cytokinesC. kill target cells directlyD. present Ag7.Which of the cytokines are secreted by Th1 cells?A. IL-4 B. IL-2 C. IL-10 D. IFN-8.Which of the diseases belong to type hypersensitivity?A. Transfusion reactionB. Arthus reactionC. Contact dermatitisD. Rheumatoid arthritis9.Which of the following is(are) correct about the characteristics of memory cells? A. Can come from either B cell or T cellB. Can respond to antigen at low consentrate effectivellyC. Only exist in HID. React to a second invasion of an antigen more rapidly and stronger10.What abilities do +T cells gain after maturation?A. self-reactiveB. self-toleranceC. express functional TCRD. self MHC restriction 11.Which of the followings are correct statements about dendritic cells?A. Can stimulate nave T cell proliferationB. Express high level of MHC class II moleculesC. Express high level of MHC class I moleculesD. Mature dendritic cells with strong function of antigen-capture 12.About AICD, which statements are correct?A. Can be mediated by FasL-Fas interactionB. Is a kind of cell death of activated T cellC. Play important roles in immunological regulationD. IL-4 can promote AICD13. The processes of antigen-activated B cells differentiation in germinal center include: A. Ig Class switchingB. Somatic hypermutation and Ig affinity maturationC. Differentiate into BmD. Apoptosis14.Which of the following factors affect the antigen immunogenecity?A. molecule weight B. foreignessC. conformation D. chemical composition15. About somatic hypermutation, which statements are correct?A. Occurs during B cells maturationB. Result in affinity maturation of antibodiesC. Mainly occurs in CDR regionD. Can occur during T cells maturationAnswer the following questions briefly (5 point for each, 30 points)得分 评卷人 1. How can T helper cells play important roles in an antibody mediated response. 扣分2.What cells express the HLA II molecules? What are the functions of HLA II molecules on these cells? 扣分3.If we want to label the antibody with enzyme for ELISA experiment, what part of antibody does enzyme should bind to? Why? 扣分4.How do effector specific CTLs kill tumor cells? What are the characteristics for this process? 扣分5. How are endogenous antigens recognized by CD8+ T cells? 扣分6. What is Neonate hemolysis? Please describe its mechanism. 扣分IV. Answer following question (12 points)得分 评卷人 1. How the innate and adaptive immunity act in cooperative and inter-dependent ways to protect the host.? 扣分The final examination of medical immunologyfor seven year students of Grade 2003(2005/12/6)Paper A I. Explain the following items (2 points for each, 18 points)1.Lymphocyte recirculation: is the process by which lymphocytes continuously move between sites throughout the body(1) through blood and lymphatic vessels, and it is critical for the initiation and effector phases of immune responses(1).2. CDR: Complementarity-determining region (0.5) the three HVR of L chain and H chain are brought together to form antigen-binding surface, so HVR is also called CDR(1)3. cytokine: A group of(0.5) low molecule weight proteins(0.5) which are secreted by activated immunocytes or some matrix cells(0.5) and take effects by binding corresponding receptor on cells(0.5). 4.MHC restriction: In interaction of T cell and antigen-presenting cell(APC) or target cell(0.5) ,T cell not only recognizes specific antigen peptide, but also recognizes polymophic residue of MHC molecules(0.5).5. Pattern recognition receptor(PRR): The receptor on macrophage(0.5) which can recognize and bind specific molecular structure on some pathogens , injured or apoptotic cells(0.5). 6. Immune tolerancea specific nonreactivity 1.0towards a given antigen 0.5in other circumstance is able to induce cell-mediated or humoral immunity 0.5 7. ITAMimmunoreceptor tyrosine-based activation motif 0.5transduct activation signal 1.0composed of two tyrosine residues separated by around 18 amino acid-when antigen binds, the tyrosine become phosphorylated by the receptor associated tyrosine kinases to transduct activatory signal 0.5 8.APCcells that can capture（0.5）, process antigen （0.5）, and present the antigenic peptide/MHC class I or II molecules（0.5） to T cells （0.5） are called antigen-presenting cells. They are also called A cell (Accessory cell). 9. TI-Ag (thymus independent Ag) : Ag which can stimulate B cells to produce Ab(0.5) without the help of T cell and M(0.5). II. Choose all correct answers (1 point for each, 15 points)No.12345678AnswerACDCDACACDBCDABDBDBDNo.9101112131415AnswerABDBCDABCABCABCDABCDABCAnswer the following questions briefly (5 point for each, 30 points)得分 评卷人 1. How can T helper cells play important roles in an antibody mediated response.(1) Express CD40L(0.5),combine with CD40 expressing on B cell(0.5),provide co-stimulation signal during the activation of B cell(1).(2) Secrete IL-4,5,6(0.5) etc. to promote proliferation and differentiation of B cell(1)(3) Secrete cytokines to promote Ig isotype switching(1.5).2.What cells express the HLA II molecules? What are the functions of HLA II molecules on these cells? cells express the HLA II molecules (2) professional APC(macrophage, dentritic cell and B cell), activated T cell, thymus epithelial cell et al.the functions of HLA II molecules (1 point each) Participate in controlling Th cell- APC recognition in immunoreaction Participate in processing and presenting of antigen Participate in inducing the differentiation and development of T cell in thymus3.If we want to label the antibody with enzyme for ELISA experiment, what part of antibody does enzyme should bind to? Why? Fc fragment(2),because:Fab binds to Ag specifically(1)Fc has no effect on Ag binding, so Fc can be labeled to detect Ag-Ab complex without effection on its Ag binding activity(2) 4.How do effector specific CTLs kill tumor cells? What are the characteristics for this process? Specific recognition and binding of target cell by CTL Lethal hit to target cell Perforin 1 Granzyme 1 Fas/FasL pathwayapoptosis 0.5 Specific killing 1 classMHC molecule restriction 1 Continuous killing of target cells in short time, and no injury of CTL 0.55. How are endogenous antigens recognized by CD8+ T cells?u By class I MHC pathway 1u Processing of endogenous Ag：proteosome -LMP2, LMP7，antigen peptides(8-13aa) 1u Transporting of antigen peptide into ER：TAP(transporter associated with antigen processing) 1u Peptide loading of MHC I molecules：ER，antigen peptideclass MHC complexes 1u Presenting to CD8+T cells：TCR-peptide, CD8-3 16. What is Neonate hemolysis? Please describe its mechanism.Definition: Maternal IgG specific for the fetal blood groups crosses the placenta and destroys the fetal red blood cells 1Mechanism:Mainly induced by Rh antigen. It is Type II hypersensitivity 0.5Rh antigens on the surface of fetal red blood cells induce IgG antibody production within mother body-the first pregnancy. 1IgG crosses the placenta and bind with Rh antigen to form Ag-Ab complex-the second pregnancy. 1Cell lysis: Ag-Ab complex