病原性球菌ppt课件PPT专业课件.ppt

1 1 Pathogeniccocci2 Otherbacteria 2 1 PathogeniccocciConceptofpathogenicbacteria13minStaphylococcus Esp S aureus 56minStreptococcus Esp S pyogenes 38minEnterococcusspeciesandVRE5minNeisseriameningitidesNeisseriagonorrhoeae13min2 OtherbacteriaPseudomonasaeruginosa10min Highlightsofcontent 3 Uptoeverypathogen thefollowingwillbetalked BiologicalpropertiesPathogenicityImmunityMicrobiologicaldetectionsPreventionandtreatment 4 Pustule 脓疱 byS aureus 5 Pustule 脓疱 byS aureus Paronychia 甲沟炎 byS aureus 6 Sorethroat 咽喉炎 byS pyogenes 7 Untreatederysipelas 丹毒 byS pyogeneswithskinsloughingandnecrosis 8 NecrotizingFasciitis 坏死性筋膜炎 byflesh eatingbacteria S pyogenes anaerobicbacteria etc andinsects bee etc stinging 9 Chestx rayFilm1justafewhoursofrigorsandproductivecough Chestx rayFilm212hourslaterconsolidationintherightupperlobe Lobarpneumonia 大叶性肺炎 byS pneumoniae 10 Meningococcalsepticemia 脑膜炎球菌性败血症 byN meningitidis widespreadecchymoses 11 Gonococcalconjunctivitis 淋菌性结膜炎 byN gonorrhoeae 12 Causativeagentsofaboveclinicaldiseases 13 WhatisPathogeniccocci alsocalled Pyogeniccocci 化脓性球菌 Including Staphylococcus S aureus S epidermidis 金黄色葡萄球菌与表皮葡萄球菌 Streptococcus GroupAStreptococci S pyogenes A群链球菌or化脓性链球菌 Streptococcuspneumoniae 肺炎链球菌 Neisseriagonorrhoeae 淋病奈瑟菌 Neisseriameningitidis 脑膜炎奈瑟菌 14 Importantcocci 15 Staphylococcusaureus ImportantpyogeniccoccusLeadingpathogenofwoundinfections includingotherstaphylococci 16 Shape Stain Culturespherical elliptical irregulargrape likeclusterGram G Growwellonbasicmedium Biologicalproperties 17 18 19 AntigenicstructureStaphylococcalproteinA SPA OnekindofsurfaceproteinincellwallSinglechainpeptideActions Binds bacterium tohost sIgG1 IgG2 IgG4coagglutinationtestCompetesw Fcreceptorsofphagocytesresistsphagocytosis 20 Coagglutinationtestwithinertparticles e g heat killedS aureusorlatexbeads 21 SPAmayresistphagocytosisbycompetivelybindingwithFcreceptorsofphagocytes 22 CapsuleTeichoicacid polysaccharide polysaccharideA S aureuspolysaccharideB S epidermidis Phasesofphagocytosis 23 ImportantPropertiesof3MainSpeciesofStaphylococci 24 Pathogenicity immunity 25 Pathogenicsubstances 26 Coagulase 27 VirulenceProtectionactionsInfectionlocalized Boundcoagulase 结合凝固酶 orclumpingfactor 凝聚因子 plasmatobeclotedorbacteriatocoagulateSlidecoagulasetest 28 classifiedinto toxin toxin makesporesincellmembranes Staphylolysin 葡萄球菌溶素 29 killsordegranulatesneutrophils macrophages 30 includingtypeA typeBcausesstaphylococcalscaldedskinsyndrome SSSS Exfoliatin 表皮剥夺毒素 31 32 33 Others heat stablenuclease 耐热核酸酶 34 35 36 Clinicaldiseases Invasivediseases Toxin mediateddiseases Pyogenicinfections localinfections systemicinfections foodpoisoning staphylococcalscaldedskinsyndrome SSSS toxicshocksyndrome TSS 37 Folliculitis 毛囊炎 byS aureus 38 BullousImpetigobyS aureus 39 AS aureusinfectionona12 year oldchild scheek 40 Pathogenesisofstaphylococcalinfections 41 Microbiologicaldiagnosis 42 Identificationofpathogenicstaphylococci Coagulase Heat stablenuclease Goldenpigment Hemolyzation Mannitefermentation 43 Prevention treatment 44 Methicillin resistantS aureus MRSA 耐甲氧西林金黄色葡萄球菌 Coagulase negativestaphylococci CNS 凝固酶阴性葡萄球菌 45 Comparethefeaturesofcommonpyogenicbacteria DiscussthepathogenicfeaturesandidentificationpointsofStaphylococcusaureus Questions 46 Streptococci 47 G spherical elliptical arrangedinchainGrowwellonnutrientmediumw blood Biologicalproperties Shape Stain Culture 48 49 50 Antigenicstructure Proteinantigen surfaceAg outsideCantigen type specific includingM T R SinGroupAPolysaccharidantigen CAg group specificNucleoprotein PAg non specific 51 Classification 52 Pathogenicity 53 GroupAStreptococci S pyogenes 54 Pathogenicsubstances 55 56 StreptolysinS SLS 链球菌溶素S lowMWglycopeptide oxygen stable causes hemolysisonBloodAgarplate non antigenic StreptolysinO SLO 链球菌溶素O proteinw SHgroup oxygen labile destroysmanykindsofcells anti SLOtest ASOtest helpfulfordiagnosis Streptolysin 57 codedbylysogenicbacteriophage proteinsincludingserotypeA B C causesscarletfever 猩红热 onekindofsuperantigens alsocausesTSS Streptococcalpyrogenicexotoxin SPE 链球菌致热外毒素 58 Streptokinase SK 链激酶 alsocalledasstreptococcalfibrinolysin 链球菌溶纤维蛋白酶 Streptodornase SD 链道酶 hydrolyzesDNA Hyaluronidase 透明质酸酶 alsocalledasspreadingfactor 扩散因子 hydrolyzeshyaluronicacidinconnectivetissue helpfulforspreadingofbacteria 59 60 Clinicaldiseases Pyogenicinfections Toxin mediateddiseases Allergicdiseases latecomplications scarletfever 猩红热 Streptococcaltoxin mediatedTSS rheumaticfever 风湿热 acuteglomeruloniphritis 急性肾小球肾炎 61 Erysipelas 丹毒 byS pyogenes 62 Cellulitis 蜂窝组织炎 byS pyogenes 63 Honey crust lesiontypicalofS pyogenesimpetigo 64 PathogenesisofS pyogenesinfections 65 Samples Pus Blood Nasopharynxswab Isolationw bloodagarplate distinguishingfromS pneumoniae Smear microscopy G streptococci distinguishingfromstaphylococciOtherstreptococci hemolysis Assist diagnosisforrheumaticfever hemolysis ASOtest Microbiologicaldiagnosis 66 S pneumoniaeGroupBstreptococciGroupDstreptococciViridans hemolytic streptococciStreptococcussuis 猪链球菌 Enterococcusspecies 肠球菌 VRE Otherstreptococci 67 EnterococcusspeciesandVRE 68 Enterococcusspp 肠球菌 PreviouslyclassifiedasGroupDstreptococciIn1984 namedasgenusEnterococcusGram cocciinpairsorshortchainsMostarefacultativeanaerobesComplexnutritionalrequirementsGroupDspecificteichoicacidantigen 69 Enterococcusspp viewedbydirectfluorescentantibody FA reaction 70 Enterococcusspp Commonlyfoundintheintestinesofnormalhealthyindividuals humansandanimals 2importanthumandiseases relatedspp E faecalis 粪肠球菌 andE faecium 屎肠球菌 CauseUrinarytractinfectionsBacteremia septicaemiaSubacuteendocarditisWoundinfectionsFoodbornediseasesMeningitisNosocomialinfections 71 Oneofimportantlife threateningnosocomialpathogensVancomycinResistantEnterococci VRE 耐万古霉素肠球菌 Since1986 VREinfectionshasbecomeagrowingproblem 72 1 DiscussthepathogenicfeaturesofStreptococcuspyogenes 2 Discussidentificationpointsbetweenhemolyticstreptococciandotherpyogenicbacteria Questions 73 Neisseriagonorrhoeae 淋病奈瑟菌 74 Biologicalproperties Shapeandstain G diplococciinoronPMNsonpussmear Noendospore flagellum w capsule pili 75 76 Culture Obligateaerobes need5 CO2whileprimaryisolation Highnutritionalrequirement ChocolateBloodAgarPlate Antigenicstructureandclassification Pilusproteinantigens Outermembraneproteinantigens PI PII PIII LOS lipooligosaccharide 脂寡糖 LPS like 77 N gonorrhoeaecoloniesonChocolateBloodAgarPlate 78 Pathogenesis Virulentfactors Pili Outermembraneproteins LOS IgA1protease Others 79 Clinicalfindings Gonorrhea Gonococcalconjunctivitis 80 Gonorrheasymptoms 81 ClinicalmanifestationsofN gonorrhoeaeinfection 82 Immunity HumoralimmunitymoreeffectiveNopersistentimmuneresponsesInfectiontrendto chronic repeatedoccurrence 83 Microbiologicaldetections Nucleicacidsdetection soon Samples Pussecretafromgenito urinarytracts eyes others Isolation OxidasetestSugarfermentationtestImmunofluorescenceassay Smear microscopy G diplococciinoronPMNs Colonies microscopicfindings Biochemicalreactions serologictests Othertests 84 Prevention treatment TakepreventionsasmainmeasureChoosepenicillinasNo 1drug takecareofproblemsofdrugs resistantbacterialstains 85 1 WhatarethemaindifferencesbetweenNeisseriameningitidesandNeisseriagonorrhoeae Question 86 Pseudomonasaeruginosa 铜绿假单胞菌 P190 87 Shape Stain CultureG bacilisingleterminalflagellumappearscapsuleins