Cell-Base ELISA试剂盒.docx

R&D systems公司出品的可取代WB的Cell-Base ELISA试剂盒检测蛋白磷酸化，可以不用天天做Western Blot。可以不用裂解细胞、不用电泳、不用转膜，可以一次检测96个样品，只需要有一台荧光酶标仪，用优宁维公司代理的R&D systems公司的Cell-Based ELISA就可以完成。该试剂盒是双荧光检测手段，可在完整细胞条件下替代传统的WB方法同时检测2个生物分子。不需要裂解细胞，更简单，更灵敏。原理如下：试剂盒组分： 与目的蛋白结合的抗体 与Normalization蛋白特异结合的抗体 HRP标记的二抗 AP标记的二抗 与HRP特异结合的F1物质 与AP特异结合的F2物 质 洗脱液 封闭液 封板膜 96孔板试剂盒优势： 不用裂解细胞 至少10,000个细胞每孔 可同时检查两种蛋白 Design allows for normalization of well-to-well variations 即用型kit，尽量减少客户优化体系 两种检测方法 Suitable for high-throughput analysis of non-adherent cells 是替代WB和双抗夹心ELISA试剂盒方法的理想选择 提供6种基础试剂盒，可任意组装自己的试剂盒如下是磷酸化EGFR cell-base elisa kit的结果示例：产品信息有：CATALOG #DESCRIPTIONSIZEKCB1095Human Phospho-EGF R (Y1068) Cell-Based ELISA1 KitKCB887Phospho-Akt (S473) Pan Specific Cell-Based ELISA1 KitKCB1935Human/Mouse Total HIF-1 alpha Cell-Based ELISA1 KitKCB1018Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Cell-Based ELISA1 KitKCB2288Human/Mouse/Rat Phospho-Histone H2AX (S139) Cell-Based ELISA1 KitKCB7226Phospho-RelA/NFkB p65 (S536) Cell-Based ELISA1 KitKCB2720Human Phospho-Tie-2 (Y992) Cell-Based ELISA1 KitKCB3226Phospho-Smad3 (S423/S425)/Smad2 (S465/S467) Cell-Based ELISA1 KitKCB4809Human/Mouse/Rat Phospho-IkB-alpha (S32/S36) Cell-Based ELISA1 KitKCB5677Human Phospho-ErbB3/Her3 (Y1262) Cell-Based ELISA1 KitKCB4607Human/Mouse Phospho-STAT3 (Y705) Cell-Based ELISA1 KitKCB2894Human Phospho-STAT1 (Y701) Cell-Based ELISA1 KitKCB7660Phospho-Smad1 (S463/S465)/Smad5 (S463/S465) Cell-Based ELISA1 KitKCB8963Human/Mouse Phospho-p70 S6 Kinase (T389) Cell-Based ELISA1 KitKCB2316Human Phospho-PDGF R beta (Y1021) Cell-Based ELISA1 KitKCB2510Human/Mouse/Rat Phospho-CREB (S133) Cell-Based ELISA1 KitKCB4418Human Phospho-ErbB4/Her4 (Y1188) Cell-Based ELISA1 KitKCB4198Human/Mouse Total COX-2 Cell-Based ELISA1 KitKCB001Cell-Based ELISA Base Kit 1 Goat Anti-Mouse IgG AlkPhos and Goat Anti-Rabbit IgG HRP Affinity Purified PAbs1 KitKCB5126Human/Mouse/Rat Phospho-FRS2 (Y436) Cell-Based ELISA1 KitKCB006Cell-Based ELISA Base Kit 6 Goat Anti-Mouse IgG HRP and Goat Anti-Rabbit IgG AlkPhos Affinity Purified Pabs1 KitKCB8871Phospho-Akt (T308) Pan Specific Cell-Based ELISA1 KitKCB3717Human/Mouse Phospho-STAT6 (Y641) Cell-Based ELISA1 KitKCB4319Human Phospho-STAT4 (Y693) Cell-Based ELISA1 KitKCB9502Human Total iNOS Cell-Based ELISA1 KitKCB4299Human Total IkB-alpha Cell-Based ELISA1 KitKCB4190Human/Mouse Phospho-STAT5a/b (Y694/Y699) Cell-Based ELISA1 KitKCB1329Human/Mouse Total beta-Catenin Cell-Based ELISA1 KitKCB4438Human Phospho-ErbB2/Her2 (Y1196) Cell-Based ELISA1 KitKCB7499Human/Mouse/Rat Phospho-c-Jun (S63) Cell-Based ELISA1 KitKCB911Human Total MMP-9 Cell-Based ELISA1 KitKCB2890Human Phospho-STAT2 (Y689) Cell-Based ELISA1 KitKCB5479Rat Phospho-TrkA (Y785) Cell-Based ELISA1 KitKCB004Cell-Based ELISA Base Kit 4 Donkey Anti-Rabbit IgG HRP and Donkey Anti-Goat IgG AlkPhos Affinity Purified Pabs1 KitKCB2114Human/Mouse Phospho-PDGF R alpha (Y742) Cell-Based ELISA1 KitKCB1205Phospho-JNK (T183/Y185) Cell-Based ELISA1 KitKCB7517Human/Mouse/Rat Phospho-Bad (S112) Cell-Based ELISA1 KitKCB705Human Cleaved Caspase-8 (Asp391) Cell-Based ELISA1 KitKCB1663Human/Mouse Total HSP70/HSPA1A Cell-Based ELISA1 KitKCB1042Mouse Phospho-PDGF R beta (Y1021) Cell-Based ELISA1 KitKCB002Cell-Based ELISA Base Kit 2 Donkey Anti-Goat IgG AlkPhos and Donkey Anti-Mouse IgG HRP Affinity Purified Pabs1 KitKCB003Cell-Based ELISA Base Kit 31 KitDonkey Anti-Mouse IgG AlkPhos and Donkey Anti-Goat IgG HRP Affinity Purified PAbsKCB005Cell-Based ELISA Base Kit 5 Goat Anti-Rabbit IgG HRP and Goat Anti-Rat IgG AlkPhos Affinity Purified Pabs1 KitKCB835Human Cleaved Caspase-3 (Asp175) Cell-Based ELISA1 KitKCB2480Human Phospho-HGF R/c-MET (Y1234/Y1235) Cell-Based ELISA1 KitKCB1947Human Phospho-MSP R/Ron (Y1238/Y1239) Cell-Based ELISA1 KitKCB1767Human Phospho-PDGF R beta (Y751) Cell-Based ELISA1 KitKCB5955Human Phospho-Progesterone R/NR3C3 (S294) Cell-Based ELISA1 KitKCB1047Human Total p21/CIP1/CDKN1A Cell-Based ELISA1 KitKCB970Human Total TIMP-1 Cell-Based ELISA1 KitKCB2314Human/Mouse Phospho-HSP27 (S78/S82) Cell-Based ELISA1 KitKCB6890Human/Mouse Phospho-PRAS40 (T246) Cell-Based ELISA1 KitKCB3776Human/Mouse Total HO-1/HMOX1 Cell-Based ELISA1 KitKCB7798Human/Mouse/Rat Phospho-Histone H3