常用载体通用引物.doc

载体名称上游引物（5primer）下游引物（3primer）P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152)pAc5.1/V5-His(_A,_B,_C)pAc5-5BGH revpAcG2TpGEX-5not availablepACT T7EEVT3/EBVrevpACT2 GAL4 ADforpGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site) PBRforBamPBRrevBampACYCDuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pACYCDuet-1(MCSII) DuetUp2T7-TerminatorpAdEasy-1not availablenot availablepAdTrack-CMVnot availablenot availablepAS2-1 GAL4 Bdfor/P24990pGAL4-BDrv/P31430pB42AD pB42ADF PB42ADRpBABE pBMN_5pBacPAK8 BAC1 BAC2pBADpBAD-FpBAD-RpBAD/HisABCpBAD-5pBBR1MCST3/M13revT7/M13forpBD-GAL4 pBD-GAL4-F PBD-GAL4-RpBI121 35S NOSpBINDGAL4-BdforT3,EBVrevpBK-CMV M13F/T7 M13R/T3pBluescriptIIKS(+/-)T3/M13revT7/M13for（优先用M13F）pBluescriptIISK(+/-)T3/M13revT7/M13forpBluescriptKS(+/-)T3/M13revT7/M13forpBluescriptSK(+/-)T3/M13revT7/M13forpBR322(BamHI-site) PBRforBamPBRrevBampBR322(EcoRI/HindIII-sites) pBRforEcopBRrevHindpBR325(BamHI-site) PBRforBamPBRrevBampBR329(BamHI-site) PBRforBamPBRrevBampBridge(MCSI)GAL4-Bdfor3BDpBridge(MCSII)not availablenot availablepBS-T IIT3/M13revT7/M13forpBS（AMP）T7T3pBSR T3 T7terpBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrevpBudCE4.1(MCSII)EF-1 FwdBGHpBudCE4.1/lacZ/CATCMV-Profor,T7c-mycrev,EBVrevpBV220 pBV220F PBV220RpCAL-cT7/Tac-ProforT7-TerminatorpCAL-nT7/Tac-ProforT7-TerminatorpCAMBIA1301(1300)pCAMBIA1301-5(ECORI)/M13RpCAMBIA1301-3(HindIII)pCAMBIA1304pCAMBIA1301-5(ECORI)pCAMBIA1301-3(HindIII)pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49)pCANTB5E S1 S6pCAT3-enhancer RVP3 此载体无反向引物pCDFDuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pCDFDuet-1(MCSII) DuetUP2 T7TERpcDNA1.1CMV-Profor,T7 pCDM8-3pcDNA2.1M13for/T7 M13rev/Tac-ProforpcDNA3T7/CMV-ProforBGH rev/SP6pcDNA3.1(+/-)pcDNA3.1F/T7/CMV-ProforBGH revpcDNA3.1/His(_A,_B,_C)T7/CMV-ProforBGH revpcDNA3.1/myc-His(_A,_B,_C)T7/CMV-ProforBGH rev/c-mycrevpcDNA3.1/V5-His(_A,_B,_C)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Hygro(+,-)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Zeo(+.-) T7BGHrevpcDNA3.3TOPOTACMV-ProforTKPAreversepcDNA4/HisMax(_A,_B,_C) XpressforBGH revpcDNA4/HisMax-TOPO XpressforBGH revpcDNA4/HisMax-TOPO/lacZ XpressforBGH revpcDNA4/myc-His(_A,_B,_C) T7,CMV-ProforBGH rev,c-mycrevpcDNA4/TOTREfor/CMV-ProforBGH revpcDNA4/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5revpcDNA4-TET/ON/AMP+CMV-ProforpcDNA5/FRTT7/CMV-ProforBGH revpcDNA6/myc-His(_A,_B,_C)CMV-Profor,T7BGH rev,c-mycrevpcDNA6/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5revpCEP4 pCEP-F/CMV-ProforEBVrevpCIT7（17BASE）EBVrevpCI-neoT7（17BASE）EBVrev,T3pCMS-EGFP T7 T3 pCMS-EGFPT7EBVrev,T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F PCMV5RpCMV6CMV-ProforpCMV6-3、HGHrevpCMV6-XL4CMV-Profor/T7pCMV6-3、HGHrevpCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrevpCMV-MYC(AMP+) PCMV-F PCMV-R,EBVrevpCMVmyc/nucCMV-ProforBGH rev,c-mycrevpcmv-scriptEXVectorT3T7pCMV-Sport6T7/M13forSP6/M13revpCOLADuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pCOLADuet-1(MCSII) DuetUP2 T7TERpCR2.1T7,M13forM13revpCR2.1-TOPOM13for,T7M13revpCR3.1T7BGH revpCR3.1-UniT7BGH revpCR4T3/M13revT7/M13forpCR4-BluntT3/M13revT7/M13forpCR4-TOPOM13rev,T3M13for,T7pCR-BluntM13revT7,M13forpCR-BluntIIM13rev/SP6T7/M13forpCR-BluntII-TOPOM13rev/SP6M13for/T7pCRIIM13rev/SP6T7/M13forpCRII-TOPOM13rev/SP6M13for/T7pCR-ScriptSK(+)T7/M13forT3/M13revpCR-XL-TOPOM13revT7,M13forpCS2+(MCSI)SP6 EBVrev,T7?pCS2+(MCSII)T3pSG5-3pDB_GAL4camT7PpDB_leuPDBLeu-5PDBLeu-3pDEST22DEST22FDEST22RpDEST15pGEX-5T7-TerminatorpDEST20pGEX-5pFastBac-3pDEST26TREfor/CMV-ProforT7/M13forpDisplayT7/CMV-Proforc-mycrevpDNR-LIB(MCS_A)M13for,T7pSG5-3,M13RpDonar M13F M13RpDONR201 PDONR-F PDONR-RpDONR207 PDONR-F PDONR-RpDONR223 M13F M13R pDriveT7/M13revSP6/M13forpDsRED1-C1 (DsRed1-N-f) DsRed1-C-rpDsRed1-N1CMV-ProforRFP-Nrev,CMV-R?pDsRed2-N1 (DsRed1-N-f) DsRed1-C-rpDsRed2-C1 (DsRed1-N-f) DsRed1-C-rpDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3pDsRed-Express dsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-RpDSRED-monomer-N1(KAN+) dsRed1_N_primerpDSRED-N1(KAN+) pEGFP-N-5 pDSRED-N-R pEASY-Blunt M13FT7/ M13RpEASY-BluntSimple M13FT7/ M13R（距离插入位点合适，优先安排）pEASY-T1 Simple M13FM13R（距离插入位点合适，优先安排）pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6pECFP-N(C) PECFP-N-5. PECFP-N-3,pEF/myc/cyto/ER/mito/nuc pEF-5 Pcdna3.1R/BGH revpEF1(4,6/myc-His)T7/pEF-5 Pcdna3.1R/BGH revpEF1(4,6/V5-His)T7/pEF-5 Pcdna3.1R/BGH revpEF1(4;6/His_A;_B;_C)T7/Xpressfor Pcdna3.1R/BGH revpEF5/FRT/V5-D(-TOPO)T7/pEF-5 Pcdna3.1R/BGH revpEGFPM13revEGFP-NrevpEGFP-1not availableEGFP-Nrev/ PEGFP-N-3pEGFP-C PEGFP-C-5 PEGFP-C-3pEGFP-C1EGFP-CforSV40-pArevpEGFP-C2EGFP-CforSV40-pArevpEGFP-C3EGFP-CforSV40-pArevpEGFP-FEGFP-CforSV40-pArevpEGFP-N PEGFP-N-5 PEGFP-N-3pEGFP-N1CMV-ProforEGFP-NrevpEGFP-N2CMV-ProforEGFP-NrevpEGFP-N3CMV-ProforEGFP-NrevpENTRM13FM13RpET-11(-a,-b,-c,-d)T7T7-TerminatorpET-12(-a,-b,-c)T7T7-TerminatorpET-14bT7T7-TerminatorpET-15bT7T7-TerminatorpET-16bT7T7-TerminatorpET-17bT7T7-TerminatorpET-17xbT7T7-TerminatorpET-19bT7T7-TerminatorpET-20b(+)T7T7-TerminatorpET-21(-a,-b,-c,-d)(+)T7T7-TerminatorpET-22b(+)T7T7-TerminatorpET-23(-a,-b,-c,-d)(+)T7T7-TerminatorpET-24(-a,-b,-c,-d)(+)T7T7-TerminatorpET-25b(+)T7T7-TerminatorpET-26b(+)T7T7-TerminatorpET-27b(+)T7T7-TerminatorpET-28a(-b,-c)(+)T7T7-TerminatorpET-29a(-b,-c)(+) S.tag/T7T7-TerminatorpET-3(-a,-b,-c,-d)T7T7-TerminatorpET-30_Ek/LICT7T7-TerminatorpET-30_Xa/LICT7T7-TerminatorpET-30a(-b,-c)(+) S.tag/T7T7-TerminatorpET-31b(+)not availableT7-TerminatorpET-32_Ek/LICTRXforT7-TerminatorpET-32_XA/LICTRXforT7-TerminatorpET-32a(-b,-c)(+) S.tag/TRXforT7-TerminatorpET-33b(+)T7T7-TerminatorpET-34b(+) S.tagT7-TerminatorpET-35b(+) S.tagT7-TerminatorpET-36b(+) S.tagT7-TerminatorpET-37b(+) S.tagT7-TerminatorpET-38b(+)not availableT7-TerminatorpET-39b(+) S.tagT7-TerminatorpET-40b(+) S.tagT7-TerminatorpET-41_Ek/LICGST-CforT7-TerminatorpET-41a(-b,-c)(+)S.tag/pGEX-5T7-TerminatorpET-42a(-b,-c)(+) S.tag/pGEX-5T7-TerminatorpET-43.1a(-b,-c)(+) S.tagcoliDOWNpET-43.1a_EK/LIC S.tagnot availablepET-44a(-b,-c)(+) S.tagT7-TerminatorpET-44a_EK/LIC S.tagnot availablepET-45b(+)T7T7-TerminatorpET-46_Ek/LICT7T7-TerminatorpET-5(-a,-b,-c)T7T7-TerminatorpET-52b(+)T7T7-TerminatorpET-7T7T7-TerminatorpET-9(-a,-b,-c,-d)T7T7-TerminatorpETcoco-1T7T7-TerminatorpETDuet-1(MCSI) pETUP1 DuetDOWN1pETDuet-1(MCSII) DuetUp2 T7-TerminatorpET-T4T7T7-TerminatorpEYFP-C1 pEGFP-C-5 pEGFP-C-3 pEYFP-N1 pEGFP-N-5,CMV-Profor pEGFP-N-3 pFLAG-ATSN26for pTrcHis-rv；C24revpFLAG-CTCN26for pTrcHis-rv；C24revpFLAG1N26for pTrcHis-rv；C24revpFLAG-CMV(-2) CMV-30；CMV-ProforCMV-24pFLAG-CMV1 CMV-30CMV-24pGAD424 GAL4_ADfor 3ADpGADGH GAL4_ADfor 3ADpGADT7 T7/GAL4_ADfor 3ADpGADT7-Rec T7/GAL4_ADfor 3AD pGADT-T T7 3,BDpGAD10GAL4_ADfor 3AD pGAPZa-A a-FACTOR 3AOX pGBKT7 T7 3,BDpGBT9MATCHMAKER5DNA-BD/GAL4BDMATCHMAKER3DNA-BDpGEM-1T7SP6pGEM-3T7SP6pGEM-3Zf(+)(-)SP6/M13revT7/M13forpGEM-4T7SP6pGEM-4ZT7/M13forSP6/M13revpGEM-5Zf(+)(-)SP6/M13revT7/M13forpGEM-7Zf(+)(-)SP6/M13revT7/M13forpGEM-9Zf(+)(-)T7/M13forSP6/M13revpGEM-TT7/M13forSP6/M13revpGEM-T_EasyT7/M13forSP6/M13revpGene/V5-His_A(_B,_C)pGENE-5BGH rev,V5revpGEX-2TpGEX-5pGEX-3pGEX-2TKpGEX-5pGEX-3pGEX-3XpGEX-5pGEX-3pGEX-4T-1(-2,-3)pGEX-5pGEX-3pGEX-5X-1(-2,-3)pGEX-5pGEX-3pGEX-6P-1(-2,-3)pGEX-5pGEX-3pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2)pGL3-Basic(MCSI)RVP3(PGL3+)?GLP2(PGL3-)?pGL3-Basic(MCSII)RVP4,PLXSN-3?pGL4pGlow(-TOPO)T7pGlow-3pGM-T T7 SP6pGWC SP6 T7pHook-2T7/CMV-Profornot availablepIND/V5-His_A(_B,_C)not availableBGH rev,V5revPinpointTMVector PinpointPrimer SP6pIRES(MCSI)T7pIRES-3pIRES(MCSII)not availableT3,EBVrevpIRES2-EGFPCMV-Profor,pIRES2-EGFP.P5 IRESrev,pIRES2-EGFP.P3pIRES-hrGFP-2aCMV-Profor,T3pIRES-hrGFP-3pIRESneo2T7/CMV-ProforpIRES-3pIVEX(all)T7/pBRrevBamT7-TerminatorpLEXA pLEXA-F pLEXA-R pLNCX pLNCX-F pLNCX-RpLNCX2 CMV-ProforpRevTRE-3pLVTHM（H1启动子下游）H1primerSP6pLXSN pLXSN-F pLXSN-R pMALc MalE primer M13F(-47) pMAL-c2E MalE primer M13F(-47)pMAL-C2x Pmal-c2x-F(P5) Pmal-C2X-R(P3 )pMALd MalE primer M13F(-47) pMALe MalE primer M13F(-47) pMAL-P4X MalE primer M13F(-47) pMAL-p2X MalE primer M13F(-47) pMET A/B/C AUG1F AUG1RpMD18-T M13R(-48)ECORI M13F(-47)(HIND)pMD19-T M13F47 M13R48pMD20-T M13F47 M13R48pMOSBlueT7/M13revM13forpMSCV-puro pLXSNfw pMSCVrevpMT/V5-His_A(_B,_C)MtforBGH rev,V5revpNTAPT3T7pCTAPpCTAP5primerT7pOTB7M13rev/T7M13for/SP6pOptiVEC-TOPO TACMV-F/CMV-ProforEMCVIRES reversepPC86 PPC86-F PPC86-RpPC97PBD-GAL4-FPBD-GAL4-RpPIC3.5K 5AOX 3AOXpPic9k(A+,Z+) 5AOX(Ppic9k-f)/a-factor 3AOX(Ppic9k-R)pPICZa 5AOX/PPICZa-F 3AOXpPROEXHTA M13R(-48) 无反向引物pProEX-HTa(b,c) M13R48/Tac-Profor pBADrv/pTrcHis-3pPD129.36M13F20/M13FpQE30or40or60(AMP+) PQE30-F PQE-30RpRC/CMV2/CATCMV/T7pRECEIVER CMV-F 此载体 无反向引物pRECEIVER-M03pRECEIVER-M03FpRECEIVER-M03RpREP4 pREP4 forward primer EBV reverse primerpRK5SP6pSG5-3pRSET T7/Xpressfor T7terpRSFDuet-1(MCSI) DuetUP1 DuetDOWN1pRSFDuet-1(MCSII) DuetUP2 T7TERpSecTac2T7/CMV-ProforBGH rev/c-mycrevpSG5pSG5-5,/T7PSG5-R(SV40)pSHlox1T7SP6pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSIT7EBVrevpSilencer1.0-U6T7T3pSilencer2.0-U6T7M13forpSilencer2.1-U6neoT7M13forpSilencer3.1-H1hygro M13F(-47) 3.0REV pSilencer4_1-CMVneopSilener4.1-5CMV-ProforpSILENCEV2.0-U6 T7 2.0REV pSIneoT7EBVrev,T3PsitT7T7TpSK01-T M13F M13R pSK-CMV(KAN+) T7 T3 pSOSPsos5Psos3pSP64SP6M13revpSP64/65 SP6 此载体无反向引物pSP65SP6M13revpSP70SP6T7pSP71SP6T7pSP72SP6T7pSP73SP6T7pSport1 SP6/M13F(-47) T7/M13R pSPORT1SP6/M13forT7/M13revpSPT18SP6T7pSTBlueT7/M13revSP6/M13forpSUPER.BasicT7/M13FM13R pSUPER T7 T3 pSURE-TM13FM13RpSURE-TsimpleM13F-47M13R-48pSuperCospBRforEcoT7pT3T7M13rev/T3M13for/T7pT7-7T7not availablepT7Blue T7 M13F(-47) pT7T3_18U M13rev/T7M13for/T3pT7T3D-PACM13rev/T7M13for/T3pTA2 M13F/T7(优先使用M13F） T3/M13RpT-AdvM13revT7,M13forpTAgT7/M13revM13forpTARGETTM pTarget.F(在T7前面）/T7 pTarget.RpTG19-TM13F(-47)M13R(-48)pTARGETTM T7/M13F PTARGET-SEQpThioHisA,B,andC Trx Forward Trx Reverse /M13forpTO-T7 T7 T7TERpTRC99A PTRC99A-F pTrcHis-rvpTrc99a-c PTRC99C-F PTRC99C-RpTRC99a-c PTRC99C-F PTRC99C-RpTrcHisB PTrcHisA-F/Xpressfor PTrcHisA-RpTrcHisA PTrcHisA-F PTrcHisA-RpT-REX-DEST30TREforT7pTriEx1T7/TriExupTriExdownpTriEx1.1T7/TriExupTriExdownpTriEx1.1_HygroT7/TriExupnot availablepTriEx1.1_NeoT7/TriExupIRESrevpTriplEX1TriplEx-LD-5T7,TriplEx-LD-3pTriplEX2TriplEx-LD-5；pTR5T7,TriplEx-LD-3pTWIN-1 T7 T7TERpTXB1T7MxeInteinrevpTXB2T7MxeInteinrevpTXB3T7MxeInteinrevpTYB1T7InteinrevpTYB2T7InteinrevpTYB3T7InteinrevpTYB4T7InteinrevpTZ_18UT7/M13revM13forpUB6/V5-HisUB-FBGH rev,V5revpUC13M13rev/M13rev(-48)M13for/M13for(-49)pUC18M13rev/M13rev(-48)M13for/M13for(-49)pUC18(19)/118(119) M13F(-47) M13R(-48)/PUC19(HIND)pUC19M13rev/M13rev(-48)M13for/M13for(-49)pUC57M13rev/M13rev(-48)M13for/M13for(-49)pUC8M13rev/M13rev(-48)M13for/M13for(-49)pUC9M13rev/M13rev(-48)M13for/M13for(-49)pUM-TM13F(-20)M13R(-20)pUCm-T(AMP+) M13F/T7 M13RpUNI/V5-Hisnot availableBGH rev,V5revpVAX1T7T7/CMV-Profor PCDNA3.1R/BGHRpVP22/mycHisVP22-CforBGH rev,c-mycrevpVP22/mycHis2T7/CMV-ProforVP22-NrevpWE15pBRrevHindT7pYES2T7pYes2.R pYES-DEST52T7/GAL1-ProforV5rev/CYC1-TerminatorpYEX_4T_1ClontechpYEX_4T-3pYEX_4T_2pGEX-5pYEX_4T-4pYEX_4T_3pGEX-5pYEX_4T-4pYEX_4T_4pGEX-5pYEX_4T-4pZeoSV2+/hu-TyrT7not availablepZERO-1M13rev/SP6T7/M13forpZERO-2M13rev/SP6M13for/T7pZome-1-CZome-FZome-RpZome-1-NZome-FZome-RYCp50(BamHI-site)PBRforBamPBRrevBamYEp13(BamHI-site)PBRforBamPBRrevBamYEp24(BamHI-site)PBRforBamPBRrevBamYIp5(BamHI-site)PBRforBamPBRrevBamRNAi-ReadypSIREN-RetroQ-U6ZsGreen U6菌PCDB T7 BGH天为时代TA载体 T7 T3m13（+） T7？ T3？pMSCV-neopMSCV-5pMSCV-3常见的通用引物序列引物名称引物序列T7TAATACGACTCACTATAGGGSP65ATTTAGGTGACACTATAG3M13F5TGTAAAACGACGGCCAGT3M13R5CAGGAAACAGCTATGACC3T7（17BASE）5ACATCCACTTTGCCTTTCTC3T7TER5TGCTAGTTATTGCTCAGCGG3S.TAG5CGAACGCCAGCACATGGACA3T35ATTAACCCTCACTAAAGGGA3M13F（47）5CGCCAGGGTTTTCCCAGTCACGAC3M13R（48）5AGCGGATAACAATTTCACACAGGA3PGEX55GGGCTGGCAAGCCACGTTTGGTG3PGEX35CCGGGAGCTGCATGTGTCAGAGG3PEGFPC55CATGGTCCTGCTGGAGTTCGTG3PEGFPC35TATGGCTGATTATGATCAGT3PEGFPN55TGGGAGGTCTATATAAGCAGAG3PEGFPN35CGTCGCCGTCCAGCTCGACCAG3PBV220F5AAGAAGGGCAGCATTCAAAG3PBV220R5CTG CGT TCT GAT TTA ATC TG35AOX5GACTGGTTCCAATTGACAAGC33AOX5GGCAAATGGCATTCTGACAT3AFACTOR5TAC TAT TGC CAG CAT TGC TGC3M13(96)5CCCTCATAGTTAGCGTAACG3PCMVF-BD5TCTAAAAGCTGCGGAATTGT3PCMVR5TCCAAACTCATCAATGTATC3P171105TACCACTACAATGGATG3P125845TTTTCAGTATCTACGAT3PAS21F5 TCATCGGAAGCGAGTAG3PAS21R5 CGTTTTAAAACCTAAGAGTCAC33AD5 AGA TGG TGC ACG ATG CAC AG33BD5TAA GAG TCA CTT TAA AAT TTG TAT C3PQE30F5 TGA GCG GAT AAC AAT TTC AC3PQE30R5 GTT CTG AGG TCA TTA CTG G3S15CAA CGT GAA AAA ATT ATT ATT CGC3S65GTA AAT GAA TTT TCT GTA TGA GG3GLP15 TGT ATC TTA TGG TAC TGT AAC TG3GLP25 CTT TAT GTT TTT GGC GTC TTC CA3RVP35 CTA GCA AAA TAG GCT GTC CC3RVP45GAC GAT AGT CAT GCC CCG CG3PTRC99CF5TTG CGC CGA CAT CAT AAC3PTRC99CR5CTG CGT TCT GAT TTA ATC TG3PIRES2EGFPF5GTA GGC GTG TAC GGT GGG AG3PIRES3EGFPR5AAC GCA CAC CGG CCT TAT TC3PCDNA3.0F25CTC TGG CTA ACT AGA GAA CC3PCDNA3.0R25GGC AAC TAG AAG GCA CAG TC3BAC15AAC CAT CTC GCA AAT AAA TA 3BAC25ACG CAC AGA ATC TAG CGC TT 3PB42ADF5CCAGCCTCTTGCTGAGTGGAGATG3PB42ADR5AAGCCGACAACCTTGATTGGAG3TRXF5CATATGAGCGATAAAATTATTCAC3TRXR5GGATCCCTTGTCATCGTCA