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1. induction of microbial cellulose(bio-cellulose)What is microbial cellulose (bio-cellulose)?Microbial cellulose is the cellulose produce from microorganism metabolism.Microorganism from the genera Aerobacter, Acetobacter, Achromobacter, Agrobacterium, Alacaligenes, Azotobacter, Pseudomonas, Rhizobium and Sarcina synthesize cellulose. However, only the Acetobacter species produce enough cellulose to justify commercial interest. The most extensively studied member of the Acetobacter species is A. xylinus, formerly known as A. xylinum. A.xylinus extrudes glucan chains from pores into the growth medium. These aggregate into microfibrils, which bundle to form microbial cellulose ribbons. If the nutrition enough for the microorganism, there will form a gel like membrane. In food industry, people call the jelly-like membrane “nata”. Nata de coco is a chewy, translucent food product produced by the bacterial fermentation of coconut water.The microbial cellulose also been used in medical device more than 10 years, and since 2002, several cosmetic manufacturer began to use this natural cellulose as essence carrier for face mask. 微生物的介绍:纤维素(生物纤维素)什么是微生物纤维素?微生物纤维素是由微生物新成代谢产生的。微生物是由来自属产气,醋酸杆菌,无菌属,农杆菌,棕色菌,假性单胞菌,根瘤菌和八叠球菌等合成的纤维素。然而,只有醋酸杆菌产生的生物纤维能产生较高的经济效益。醋酸杆菌的物种中被广泛研究的一个成员是A杆菌,A挤压毛孔的葡聚糖链入培养基。这些生物微纤维,捆绑成一个带状纤维素结构。如果有足够的营养,它们将形成一个凝胶样的膜。在食品领域,人们称这种果冻状的膜为“纳塔”。 椰果纳塔是一种耐嚼,半透明的,由椰子水细菌发酵生产的食品。微生物纤维素也被用在医疗设备已有10余年,自2002年以来,一些化妆品生产商开始使用天然纤维素载体作为面膜的承载精华液的载体。Process of Microbial cellulose produce by Acetobacter 醋酸菌产生微生物纤维的过程:Among the bacteria, one of the most advanced types of purple bacteria is the common vinegar bacterium, Acetobacter. This non-photosynthetic organism can procure glucose, sugar, glycerol, or other organic substrates and convert them into pure cellulose). 这些细菌中,紫色细菌中最为活跃的一种是常见的醋细菌,醋酸菌。这些非光和作用菌类可从葡萄糖,蔗糖,甘油或其他有机质中获得并把它们变成纯粹的生物纤维。Acetobacter xylinum is Natures most prolific cellulose-producing bacterium. 木醋杆菌是大自然最丰富的纤维素产生菌。Consider that as many as a million cells can be packed into a large liquid droplet, and if each one of these factories can convert up to 108 glucose molecules per hour into cellulose, the product should virtually be made before ones eyes. 假如,数以百万计的细胞可以被包装成一个大的水滴,而且每一个这个工厂每小时把108个多糖分子发酵成纤维素,那么,成品就成功的展现在眼前了。A single cell of Acetobacter has a linear row of pores from which glucan chain polymer aggregates are spun As many as one hundred of these pores can produce a composite cable of glucan polymers resulting in a ribbon. 醋酸菌的一个单细胞有一可以产生葡聚糖链的线性排气孔,只需要其中的一个小孔就可以产出,就像100这样的孔可以产生一个带状的类似复合电缆的线性葡聚糖链。Time lapse analysis of individual Acetobacter cells assembling cellulose ribbons reveals a myriad of activities, each cell acting as a nano-spinneret, producing a bundle of sub-microscopic fibrils.对粘附着链状纤维素的单个醋酸菌细胞的时间错位分析揭示了一系列的活动,每个细胞都发挥着纳米喷丝板的作用,产生了一个亚微观7的纤维束。Together, the entangled mesh of these fibrils produces a gelatinous membrane known as a pellicle.同时,由这些纤维缠绕的网产生了一种胶状的膜,称之为薄膜。 This membrane of pure cellulose, and cells entrapped within it can be cleaned and dried and the product used for many exciting new applications. 纯纤维素的这种膜,以及它所包裹在内的细胞,都可以清洗、晾干,而且这种产品还被用于很多令人兴奋的新品实验中。One of the unique features of this pure cellulose membrane is that it is very strong in the never dried state, and it can hold hundreds of times its weight in water. 这种纯的生物纤维薄膜的特色之一便是它在干燥的环境中有很强的生命力,并且他能够承载终于它自身几百倍的水分。This great absorbtivity and strength constitute two of the many novel features of microbial cellulose. 强大的吸水性和生命力这两个特点决定了生物纤维的很多特色。 The tiny cellulose produce from Acetobacteria cell微小的纤维产自于醋酸菌细胞Structure of Microbial cellulose (Bio-cellulose)生物纤维面膜的结构 Cultivation of Microbial Cellulose Processed Microbial Cellulose微生物纤维的培养 已经形成的微生物纤维 Raw product of Microbial Cellulose SEM image of Microbial Cellulose微生物纤维的初级产品 微生物纤维的扫描电镜图像 Basic composition of Microbial Cellulose (-1,4-glucan chain)微生物纤维的基本构成图Properties of Microbial cellulose membrane微生物纤维薄膜的特点:1.high water holding capacity (typical membrane can hold up to 200 g of its dry mass in water); high water vapor transmission rate1.超强的储水功能(一般的薄膜,把干膜放在水中,可以储存200 g),特强的水汽转水率2. nanoporous structure does not allow any external bacteria to penetrate into the wound bed2.纳米多孔结构能有效地阻止任何来自外界的细菌的入侵伤口表层,3. partially dehydrated membrane is able to absorb fluid up to its original capacity. Physical processing of the membrane (i.e., squeezing) can remove part of the initial water and allow the membrane to be more absorbable3.部分脱水的膜亦能够液体至其原有的容量。这个薄膜的物理过程(例如挤压)也可以排除部分最初的水并让薄膜本身变得更具吸附力。4. high elasticity and conformability,4.高弹性和适应性5. biocompatible, nonpyrogenic, nontoxic5.生物相容性,无热原性和无毒性6. high mechanical strength6.极强的力学性Different products from microbial cellulose生物纤维的不同产品Food industry食品工业:Desserts (Nata de Coco, low calorie ice creams chips, snacks, candies)甜点(椰果纳塔,低卡里冰激凌条,点心,糖果)Thickeners (ice cream and salad dressing)增稠剂(冰激凌和沙拉酱)Weight reduction base减肥基本成分Base for artificial meat人造肉的基本成分Sausage and meat casings香肠和肉类的肠衣Serum cholesterol reduction (see US Patent 4,960,763)降低胆固醇血清(见美国专利4,960,763号)Kombucha elixir or Manchurian tea红茶菌或东北茶菌制剂Healthcare/ medical device健康和医疗Wound care dressings (see patent EP 323717)伤口敷料(见专利EP323717)Drug delivery agents, either oral or dermal 口服或外用药的药品作用剂Artificial skin substrate 人工皮肤的有机培养基Artificial blood vessels 人工血管的有机培养基Cosmetics and Beauty化妆品和美容产品Skin care membrane product (bio-cellulose mask and eyes pad)护肤膜类产品(生物纤维面膜和眼膜)Skin creams 护肤霜Astringents 收敛剂Base for artificial nails 人工指甲的主要成分Thickener and strengthener for fingernail polish 指甲油的粘稠剂和强化剂Environmental 环境保护Oil spill cleanup sponge 去油污海绵Absorptive base for toxic material removal 去除有毒物质的吸附剂Public Utilities 公共事业Water purification via ultrafilters and reverse osmosis membranes (see patent by Nakano Sumise JP 3032726)过滤水净水膜的和反渗透膜(见日本Nakano Sumise专利 3032726)Audio Products 音频设备Superior audio speaker diaphragms高级音频扬声器抗振膜Specialty Papers 特殊纸品Archival document repair 档案文件修复Paper base for long-lived currency 长寿命纸币的用纸Advantages of microbial cellulose in skin care use over other materials生物纤维在护肤品方面的运用远超过其他材料 Finer structure, very comfortable to skin 更好的结构,非常适宜皮肤 Can completely attach to the skin and deliver the functional ingredient 可以完全接触皮肤,并有效地传送功能性原液 Pure natural cellulose and nontoxic, nonpyrogenic, and biocompatible 纯天然纤维,无毒,无热原,不会引起过敏反应 Can be utilized in any shape可以任何形状使用 Able to control fluid loss then elongate the reaction between functional ingredients and skin and helps the water soluble ingredients pass through the lipid-rich tratum Corneum. 能够控制水分流失,延长皮肤与功能性原液之间的反应,帮助水溶性成分成功通过脂质很厚的角质层。2.Microbial cellulose (bio-cellulose) mask produce process生物纤维面膜的生产过程:2.Cultivation of Acetobacteria (stirring status)醋酸菌的培养(萌芽状态)1. Fermentation of coconut juice by yeast 由椰子汁发酵成椰汁酵母by3. Fermentation of medium by Acetobacteria (static)醋酸菌的发酵培养基(静止的) 4. Harvest(发酵结果) 5. Raw microbial cellulose de-microorganism微生物纤维的初级成品 6. Fold the microbial cellulose and non-woven paper 折好生物纤维膜和无纺布7. Squeeze out the un- necessary liquid from membrane 从膜中挤出不必要的液体8. Cutting the membrane to the needed shapes把膜切成需要的形状9. QC for shaped membrane已成型的膜的质检10. package包装11. Packed material sterilized by autoclave 高压灭菌器杀过菌的包装材料12. Final QC to prevent bacteria contamination最后的防治污染的质检Cultivation of Acetobacteria醋酸菌的培养3. brands who use bio-cellulose for mask material all over the world3.世界上用生物纤维做面膜的品牌:a. Lancome .tw/_zh/_tw/catalog/product1.aspx?prdcode=110359&categorycode=AXESkincareF1_SpecialcareF2_Spe_Mask&vname=name&b. Dr. Brandt (USA) /about/dr_brandtc. For Beloved one (Taiwan) /product.php?TVcode=1&TNO=1&NO1=4d. Majiami (Taiwan) .tw/pro_black_mask.htmle. KOZI (Taiwan) .tw/bio.htmlSince 2002, more and more cosmetic companies replaced their original mask essence carrier materials to microbial cellulose (bio-cellulose). Above brands are onl
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