pPic9K质粒全序列(来自NCBI数据库).doc

LOCUSpPIC9K9276 bpDNASYN10-Dec-2011DEFINITIONpPIC9KACCESSIONKEYWORDSSOURCEORGANISMother sequences; artificial sequences; vectors.FEATURESLocation/Qualifierssource1.9276/organism=pPIC9K/mol_type=other DNAmisc_feature1152.1172/label=alpha_factor_primermisc_featurecomplement(1327.1347)/label=3_AOX1_primergenecomplement(1650.1937)/label=tet(288-0)/gene=tet(288-0)CDScomplement(1980.4514)/label=HIS4/gene=HIS4/note=ORF frame 3/translation=MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTELQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTLVDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPIIDARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSIPEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLERTSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKEDIAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKPKEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLELTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCPGVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPEVIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSALCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIEDAVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQVQHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLKHIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI*genecomplement(1980.4514)/label=HIS4/gene=HIS4CDScomplement(4928.5743)/label=KanR2/gene=KanR2/note=ORF frame 2/translation=MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*genecomplement(4928.5743)/label=KanR2/gene=KanR2misc_featurecomplement(5908.5927)/label=pBRrevBam_primerCDScomplement(5909.6631)/label=ORF frame 2/translation=MTSKHEHTIVSFKTALAWINSRQTTLISLDSEWFERNSNVVTEVGISIYKPYIPLEGPEGKHLPAPFLPDISTLHFIVQGTEKVRNKKFVPDNKDFFIGGTSFTVSKKDISAVITEIVSQFESTDDQKSEKFTMKSPPPVALVGHNLIGDLKTLKNAGITIPILPIIDTRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPTPKQALMSPKWRARSSPSVMSAI*misc_featurecomplement(6913.6935)/label=pGEX_3_primerrep_origincomplement(7332.7951)/label=pBR322_originCDScomplement(8106.8966)/label=Ampicillin/gene=Ampicillin/note=ORF frame 3/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*genecomplement(8106.8966)/label=Ampicillin/gene=Ampicillinpromotercomplement(9008.9036)/label=AmpR_promoterORIGIN1AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG61GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT121TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC181AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA241ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA301TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG361AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT421GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG481TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT541CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT601CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT661TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT721GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT781ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT841ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA901CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAAACGAT GAGATTTCCT961TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT1021ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA1081GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG1141TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAA1201AGAGAGGCTG AAGCTTACGT AGAATTCCCT AGGGCGGCCG CGAATTAATT CGCCTTAGAC1261ATGACTGTTC CTCAGTTCAA GTTGGGCACT TACGAGAAGA CCGGTCTTGC TAGATTCTAA1321TCAAGAGGAT GTCAGAATGC CATTTGCCTG AGAGATGCAG GCTTCATTTT TGATACTTTT1381TTATTTGTAA CCTATATAGT ATAGGATTTT TTTTGTCATT TTGTTTCTTC TCGTACGAGC1441TTGCTCCTGA TCAGCCTATC TCGCAGCTGA TGAATATCTT GTGGTAGGGG TTTGGGAAAA1501TCATTCGAGT TTGATGTTTT TCTTGGTATT TCCCACTCCT CTTCAGAGTA CAGAAGATTA1561AGTGAGAAGT TCGTTTGTGC AAGCTTATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT1621TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC ATCGTCATCC1681TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT TATGCCGGTA CTGCCGGGCC1741TCTTGCGGGA TATCGTCCAT TCCGACAGCA TCGCCAGTCA CTATGGCGTG CTGCTAGCGC1801TATATGCGTT GATGCAATTT CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG1861GCCGCCGCCC AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG1921CGACCACACC CGTCCTGTGG ATCTATCGAA TCTAAATGTA AGTTAAAATC TCTAAATAAT1981TAAATAAGTC CCAGTTTCTC CATACGAACC TTAACAGCAT TGCGGTGAGC ATCTAGACCT2041TCAACAGCAG CCAGATCCAT CACTGCTTGG CCAATATGTT TCAGTCCCTC AGGAGTTACG2101TCTTGTGAAG TGATGAACTT CTGGAAGGTT GCAGTGTTAA CTCCGCTGTA TTGACGGGCA2161TATCCGTACG TTGGCAAAGT GTGGTTGGTA CCGGAGGAGT AATCTCCACA ACTCTCTGGA2221GAGTAGGCAC CAACAAACAC AGATCCAGCG TGTTGTACTT GATCAACATA AGAAGAAGCA2281TTCTCGATTT GCAGGATCAA GTGTTCAGGA GCGTACTGAT TGGACATTTC CAAAGCCTGC2341TCGTAGGTTG CAACCGATAG GGTTGTAGAG TGTGCAATAC ACTTGCGTAC AATTTCAACC2401CTTGGCAACT GCACAGCTTG GTTGTGAACA GCATCTTCAA TTCTGGCAAG CTCCTTGTCT2461GTCATATCGA CAGCCAACAG AATCACCTGG GAATCAATAC CATGTTCAGC TTGAGACAGA2521AGGTCTGAGG CAACGAAATC TGGATCAGCG TATTTATCAG CAATAACTAG AACTTCAGAA2581GGCCCAGCAG GCATGTCAAT ACTACACAGG GCTGATGTGT CATTTTGAAC CATCATCTTG2641GCAGCAGTAA CGAACTGGTT TCCTGGACCA AATATTTTGT CACACTTAGG AACAGTTTCT2701GTTCCGTAAG CCATAGCAGC TACTGCCTGG GCGCCTCCTG CTAGCACGAT ACACTTAGCA2761CCAACCTTGT GGGCAACGTA GATGACTTCT GGGGTAAGGG TACCATCCTT CTTAGGTGGA2821GATGCAAAAA CAATTTCTTT GCAACCAGCA ACTTTGGCAG GAACACCCAG CATCAGGGAA2881GTGGAAGGCA GAATTGCGGT TCCACCAGGA ATATAGAGGC CAACTTTCTC AATAGGTCTT2941GCAAAACGAG AGCAGACTAC ACCAGGGCAA GTCTCAACTT GCAACGTCTC CGTTAGTTGA3001GCTTCATGGA ATTTCCTGAC GTTATCTATA GAGAGATCAA TGGCTCTCTT AACGTTATCT3061GGCAATTGCA TAAGTTCCTC TGGGAAAGGA GCTTCTAACA CAGGTGTCTT CAAAGCGACT3121CCATCAAACT TGGCAGTTAG TTCTAAAAGG GCTTTGTCAC CATTTTGACG AACATTGTCG3181ACAATTGGTT TGACTAATTC CATAATCTGT TCCGTTTTCT GGATAGGACG ACGAAGGGCA3241TCTTCAATTT CTTGTGAGGA GGCCTTAGAA ACGTCAATTT TGCACAATTC AATACGACCT3301TCAGAAGGGA CTTCTTTAGG TTTGGATTCT TCTTTAGGTT GTTCCTTGGT GTATCCTGGC3361TTGGCATCTC CTTTCCTTCT AGTGACCTTT AGGGACTTCA TATCCAGGTT TCTCTCCACC3421TCGTCCAACG TCACACCGTA CTTGGCACAT CTAACTAATG CAAAATAAAA TAAGTCAGCA3481CATTCCCAGG CTATATCTTC CTTGGATTTA GCTTCTGCAA GTTCATCAGC TTCCTCCCTA3541ATTTTAGCGT TCAACAAAAC TTCGTCGTCA AATAACCGTT TGGTATAAGA ACCTTCTGGA3601GCATTGCTCT TACGATCCCA CAAGGTGGCT TCCATGGCTC TAAGACCCTT TGATTGGCCA3661AAACAGGAAG TGCGTTCCAA GTGACAGAAA CCAACACCTG TTTGTTCAAC CACAAATTTC3721AAGCAGTCTC CATCACAATC CAATTCGATA CCCAGCAACT TTTGAGTTGC TCCAGATGTA3781GCACCTTTAT ACCACAAACC GTGACGACGA GATTGGTAGA CTCCAGTTTG TGTCCTTATA3841GCCTCCGGAA TAGACTTTTT GGACGAGTAC ACCAGGCCCA ACGAGTAATT AGAAGAGTCA3901GCCACCAAAG TAGTGAATAG ACCATCGGGG CGGTCAGTAG TCAAAGACGC CAACAAAATT3961TCACTGACAG GGAACTTTTT GACATCTTCA GAAAGTTCGT ATTCAGTAGT CAATTGCCGA4021GCATCAATAA TGGGGATTAT ACCAGAAGCA ACAGTGGAAG TCACATCTAC CAACTTTGCG4081GTCTCAGAAA AAGCATAAAC AGTTCTACTA CCGCCATTAG TGAAACTTTT CAAATCGCCC4141AGTGGAGAAG AAAAAGGCAC AGCGATACTA GCATTAGCGG GCAAGGATGC AACTTTATCA4201ACCAGGGTCC TATAGATAAC CCTAGCGCCT GGGATCATCC TTTGGACAAC TCTTTCTGCC4261AAATCTAGGT CCAAAATCAC TTCATTGATA CCATTATTGT ACAACTTGAG CAAGTTGTCG4321ATCAGCTCCT CAAATTGGTC CTCTGTAACG GATGACTCAA CTTGCACATT AACTTGAAGC4381TCAGTCGATT GAGTGAACTT GATCAGGTTG TGCAGCTGGT CAGCAGCATA GGGAAACACG4441GCTTTTCCTA CCAAACTCAA GGAATTATCA AACTCTGCAA CACTTGCGTA TGCAGGTAGC4501AAGGGAAATG TCATACTTGA AGTCGGACAG TGAGTGTAGT CTTGAGAAAT TCTGAAGCCG4561TATTTTTATT ATCAGTGAGT CAGTCATCAG GAGATCCTCT ACGCCGGACG CATCGTGGCC4621GACCTGCAGG GGGGGGGGGG GCGCTGAGGT CTGCCTCGTG AAGAAGGTGT TGCTGACTCA4681TACCAGGCCT GAATCGCCCC ATCATCCAGC CAGAAAGTGA GGGAGCCACG GTTGATGAGA4741GCTTTGTTGT AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT4801GCGTTGTCGG GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG ATTTATTCAA4861CAAAGCCGCC GTCCCGTCAA GTCAGCGTAA TGCTCTGCCA GTGTTACAAC CAATTAACCA4921ATTCTGATTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT4981TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC5041AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA5101TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG5161TGACGACTGA ATCCGGTGAG AATGGCAAAA GCTTATGCAT TTCTTTCCAG ACTTGTTCAA5221CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC5281GTGATTGCGC CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG5341GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT5401CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC5461ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA5521GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT5581TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT5641GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA5701ATCGCGGCCT CGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC5761TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGATGATAT ATTTTTATCT TGTGCAATGT5821AACATCAGAG ATTTTGAGAC ACAACGTGGC TTTCCCCCCC CCCCCTGCAG GTCGGCATCA5881CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC5941GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG6001TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC ATTCCTTGCG GCGGCGGTGC6061TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC6121GTCGAGTATC TATGATTGGA AGTATGGGAA TGGTGATACC CGCATTCTTC AGTGTCTTGA6181GGTCTCCTAT CAGATTATGC CCAACTAAAG CAACCGGAGG AGGAGATTTC ATGGTAAATT6241TCTCTGACTT TTGGTCATCA GTAGACTCGA ACTGTGAGAC TATCTCGGTT ATGACAGCAG6301AAATGTCCTT CTTGGAGACA GTAAATGAAG TCCCACCAAT AAAGAAATCC TTGTTATCAG6361GAACAAACTT CTTGTTTCGA ACTTTTTCGG TGCCTTGAAC TATAAAATGT AGAGTGGATA6421TGTCGGGTAG GAATGGAGCG GGCAAATGCT TACCTTCTGG ACCTTCAAGA GGTATGTAGG6481GTTTGTAGAT ACTGATGCCA ACTTCAGTGA CAACGTTGCT ATTTCGTTCA AACCATTCCG6541AATCCAGAGA AATCAAAGTT GTTTGTCTAC TATTGATCCA AGCCAGTGCG GTCTTGAAAC6601TGACAATAGT GTGCTCGTGT TTTGAGGTCA TCTTTGTATG AATAAATCTA GTCTTTGATC6661TAAATAATCT TGACGAGCCA AGGCGATAAA TACCCAAATC TAAAACTCTT TTAAAACGTT6721AAAAGGACAA GTATGTCTGC CTGTATTAAA CCCCAAATCA GCTCGTAGTC TGATCCTCAT6781CAACTTGAGG GGCACTATCT TGTTTTAGAG AAATTTGCGG AGATGCGATA TCGAGAAAAA6841GGTACGCTGA TTTTAAACGT GAAATTTATC TCAAGATCTC TGCCTCGCGC GTTTCGGTGA6901TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC6961GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG7021CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA7081TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA7141AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG7201GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA7261GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC7321CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC7381AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG7441TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC7501CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT7561CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG7621CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC7681TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT7741GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT7801ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC7861AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA7921AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC7981GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC8041CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT8101GACAGTTACC AATGCTTAA