xray crystallography facility overviewklb型x射线晶体学设施概述课件

Welcome X RayFacility 410 413KashaLaboratoryInstituteofMolecularBiophysicsFloridaStateUniversityTallahassee FL32306 4380www sb fsu edu xray ThayumanasamySomasundaram 2003 2004 2 27 2020 2 X RayFacilityOrientation HistoryoftheFacilityFacilityResourcesRequirementsDataCollectionDataProcessing ManualsArchiving SummaryContact Information ThayumanasamySomasundaram 2003 2004 2 27 2020 3 HistoryoftheFacility 1 Corefacilityplannedin1990 92FacilityDirectorhiredFeb1993ConstructionJune Sep1993Twogenerators ImagePlate IP detectorinJanuary1994Elliottgeneratorin1995 ImportantDates ThayumanasamySomasundaram 2003 2004 2 27 2020 4 HistoryoftheFacility 2 Cryocapabilitiesin1996Crystalchamberin1999ConfocalmirrorinSpring2000CCDw confocalmirrorinFall2000CryoCooler700w auto refillinFall2000AdditionalLinuxmachinesin2000Completerenovationduring2002 03 ImportantDates ThayumanasamySomasundaram 2003 2004 2 27 2020 5 FacilityResources Accessories ThreeCopperrotatinganodegeneratorsTwoautomatedareadetectorsTwoconfocal onetotalreflectionmirrorsTwocryocoolersOneautomatedliquidNitrogenrefillerLinuxandWindowsNTcomputersFulltimeFaculty Staff State line ThayumanasamySomasundaram 2003 2004 2 27 2020 6 Resources 1 GeneratorsTwoRigakuH2R5 4kWgeneratorsOneElliottGX 303 0kWgeneratorAreadetector 1 IP R AxisIIc 1994 w confocalbluemirror65 450mmsample detectordistance0 25degreenon zero2 thetacapabilityOxfordCryostream600coldhead ThayumanasamySomasundaram 2003 2004 2 27 2020 7 Resources 2 Areadetector 2 CCD Mar165CCDw confocalpurplemirror35 380mmsample CCDdistanceLimitednon zero2 thetacapabilityOxfordCryostream700coldheadLong shortSupper total reflection mirrorsStand aloneIPBioUnitIAnalyticalLab ThayumanasamySomasundaram 2003 2004 2 27 2020 8 Accessories 1 Leicastereomicroscopew MoticamdigitalcameraNikonOptiphotmicroscopew analogcameraStereozoommicroscopesDVD R RwritersDDS 3 4tapedrivesFireWire Maxtor 60 80GB drivesWindowsNTandfiveLinuxcomputers ThayumanasamySomasundaram 2003 2004 2 27 2020 9 Accessories 2 Coldroomfor4 Ccrystallizationset upRoomforambientcrystallizationset upCrystallizationchamberTemperature humiditymonitoringDryCryoShipperLong termsamplestorageLaptopforsynchrotrontrips ThayumanasamySomasundaram 2003 2004 2 27 2020 10 Requirements MemberofStructuralBiologyProgramRadiationsafetytrainingComputeraccount UNIX LINUX NT Bodyand orfingerradiationbadgeNocosttouseUsersbringconsumables CryoLoops Capillaries TapeandDVDmedia ThayumanasamySomasundaram 2003 2004 2 27 2020 11 DataCollection 1 SamplemountingdecisionCapillaryorloopmounting DatacollectiontemperatureAmbientorcryo Ifcryo cryoprotectionselectionGroworsoakcrystal DetectordecisionImageplateorCCDdetector ThayumanasamySomasundaram 2003 2004 2 27 2020 12 DataCollection 2 Screeningorregulardatacollection Improvementorbrand newcrystal ResolutiondecisionLow moderate orhigh resolution 2 thetaangleCelldimension detectordistance ThayumanasamySomasundaram 2003 2004 2 27 2020 13 DataCollectionProcedure Checkthescheduleat www sb fsu edu soma XraySchedules schfrmset htmlRequesttimebye mail soma sb fsu edubyphone 644 6448PrioritygivenforsamplesinthebeamPrioritygiventounstablesamplesFlexibleschedule co operationisvalued ThayumanasamySomasundaram 2003 2004 2 27 2020 14 DataProcessingProcedure Collectpreliminarydata autoindexIfsuccessful completedatacollectionIfnot modifyprocedureandtryIndex integrate scalefulldatasetRepeatintegration scaling ifneededValidateandcompareArchive transferfiles ThayumanasamySomasundaram 2003 2004 2 27 2020 15 Archiving Dataistheresponsibilityofthe USER Availablearchivingresources DDS 3 DDS 4tapedrivesDVD R RwritersUsersbringthemediaFacilityarchivesdata lastresort ComputerResourcesmaybehelpful ThayumanasamySomasundaram 2003 2004 2 27 2020 16 Manuals Documents Onlineversionathttp www sb fsu edu xray Manuals soft man htmlPaperversionin410KLB XRF Programauthors websiteManufacturer swebsiteIMBresourcepagesLabprotocolsandprocedures ThayumanasamySomasundaram 2003 2004 2 27 2020 17 Summary StateoftheartfacilityExcellentscientific supportstaff ComputerResources ProteinExpressionFacility PhysicalBiochemFacility Electronicsshop MachineshopKeepitcleanandinworkingorderReportproblemstoSoma 4 6448 ThayumanasamySomasundaram 2003 2004 2 27 2020 18 Contacts Information 1 Datacollection processing scheduling ThayumanasamySomasundaram SOMA Ph 644 6448 E mail soma sb fsu eduwww sb fsu edu soma www sb fsu edu xrayXRF KLB410 413 645 1333Computerrelated MikeZawrotnyPh 644 0069 zawrotny sb fsu edu ThayumanasamySomasundaram 2003 2004 2 27 2020 19 Contacts Information 2 Electronics RickWangPh 645 3309 wang sb fsu eduMachineShop Woodshop WadeBaggettorEdKirklandPh 644 1419 645 1338 ThayumanasamySomasundaram 2003 2004 2 27 2020 20 PictureGallery IP CCDDetectorsIMB 2001 ThayumanasamySomasundaram 2003 2004 2 27 2020 21 Pict