国外食品检验教程英文.ppt

TrendsandNeedsinFoodDiagnostics LarissaKahr BIT AustriaMalinBr nnback TEKES FinlandGabrielavonBlankenfeld Enkvist TEKES Finland BureauforInternationalResearchandTechnologyCooperation Austria AimoftheStudy InvestigatetechnologicaltrendsandneedsinFoodDiagnosticsUnderstandthefactorsthatshapeandinfluencethedevelopmentofthisfield BureauforInternationalResearchandTechnologyCooperation Austria Focus BackgroundonfoodsafetymanagementtoolsDescriptionofthemethodscurrentlyusedandnewmethodsdevelopedThemarketforfooddiagnostics BureauforInternationalResearchandTechnologyCooperation Austria Definitions FoodDiagnosticsappliesmodernmethodsdevelopedinhumaninvitrodiagnostictofoodRapidMethodscomprisesdifferenttypesoftestsMiniaturisedbiochemicalkitsAntibody basedtestsNucleicacidhybridisation basedtestsManual semi automatedorfullyautomated BureauforInternationalResearchandTechnologyCooperation Austria Detection Bacteria viruses parasites chemicals biotoxins heavymetalsandprionsInallstepsofthefoodchain fromrawmaterialstoendproducts BureauforInternationalResearchandTechnologyCooperation Austria Foodastestmatrix ExtremelyvariedinitschemicalcompositionContainsmanydifferentingredientsMightcontainanintrinsicmicrobialflora BureauforInternationalResearchandTechnologyCooperation Austria FrameFoodSafety ProcesscontrolismoreefficientthanproductcontrolThesafetyofaproductwithlowdefectratescannotbeguaranteedthroughsamplingandtesting Thefocusischangingfromend producttestingtoHACCPThishastobeconsideredwhendevelopingnewmethodswithintheconceptualframeoffoodsafety BureauforInternationalResearchandTechnologyCooperation Austria Microbiologicalmethodscanbedividedinto traditional or conventional methodsandrapidmethods BureauforInternationalResearchandTechnologyCooperation Austria ConventionalMethods ConventionalmethodsrelyonculturingmicroorgansimsontoagarplatesLaboriousandtime consuming84 ofalltestsarebasedoncountingviablecellsRequiredbynationalandinternationalregulatoryagenciesforofficialcontrol BureauforInternationalResearchandTechnologyCooperation Austria RapidMethods AdvantagesinanalysistimePossibilitytoeliminatelabourintensivestepsPotentialforautomationHowever lengthyincubationproceduresarestillnecessaryFirstone shiftmethodsunderdevelopmentELISAandPCRaredominatingLackofacommonvalidationprocedureMostlynotacceptedforofficialcontrolpurposes BureauforInternationalResearchandTechnologyCooperation Austria Methods AdvancesinViableCellCountMethodsMiniaturisationandDiagnosticKits BiochemicalIdentificationTechniquesAntibody basedmethodsImmunomagneticSeparationNucleicacid basedassaysBiosensorsMicroarraysFlowCytometryBacteriophage BasedTechniquesATPbioluminescenceAdenylatekinasebioluminescenceRiboprintingandPulse FieldGelElectrophoresis BureauforInternationalResearchandTechnologyCooperation Austria Viablecellcounting Willremainimportantfortheassessmentofsafetyandqualityoffoodproductsdevelopmentofsemi automatedalternativemethodsnewmethodsarebasedongrowthandmetabolicactivityMicroscopicmethodsflowcytometryandcellsorting FACS BureauforInternationalResearchandTechnologyCooperation Austria Immunoassays ELISA withdipsticktechnology ImmunochromatographyFullyautomatedsystemsalreadyonthemarketNewrecombinantantibodiesandmolecularimprintingtechniquestoimprovesensitivityandversatility BureauforInternationalResearchandTechnologyCooperation Austria ImmunomagneticSeparation SpeedupenrichmentstepsNodetectionmethoditselfCombinationwithdifferentend detectionmethodstoimprove Speedandsensitivity Samplethroughput Userfriendliness Automationexpectedinthenextfuture BureauforInternationalResearchandTechnologyCooperation Austria Nucleicacidbasedassay DirectlyorafteramplificationwithPCRDevelopmentofhomogenousassaysandautomationimprovesroutineuseandreducesfalsepositiveresultsafterPCRNewassayformatsunderdevelopment BureauforInternationalResearchandTechnologyCooperation Austria BiosensorsandMicroarrays On lineoron site sensitive low cost rapidmethodsforroutine usePrototypesunderdevelopmentHavehighpotentialforautomationSimpleandportableequipmentcanbedesignedApplicationswithinqualityandprocesscontrol controloffermentationprocesses qualityandsafetycontrolofrawmaterialsandforHACCPmonitoring BureauforInternationalResearchandTechnologyCooperation Austria Hygienemonitoring NeedtodeveloprealtimemethodstomonitorcleaningprocedureMethodsshouldbecheap robustATPbioluminescence Adenylatekinase biosensorapplicationsmightbefurtherdeveloped BureauforInternationalResearchandTechnologyCooperation Austria MethodsImplementation Inhousealternativemethodsforviablecellcountshygienemonitoringchemicalanalysis Outsourcingpathogentesting BureauforInternationalResearchandTechnologyCooperation Austria TheMarketforFoodDiagnosticsDefinitions FoodAnalysis allfoodtestingFoodDiagnostics foodtestingwithrapidmethodsMicrobiologicalTesting alltestingformicroorganismsFoodMicrobiologicalTesting alltestingformicroorganismsinfoodandbeverages BureauforInternationalResearchandTechnologyCooperation Austria TheMarketforFoodDiagnosticsSomefigures Foodanalysisglobalmarket 1 1billionSharedbyabout50companiesUSAandEuropemakeupabout2 3 565millionglobalsalesforFoodmicrobiologicaltestsRapidmethods 115millionora20 totalmarketshare BureauforInternationalResearchandTechnologyCooperation Austria BureauforInternationalResearchandTechnologyCooperation Austria BureauforInternationalResearchandTechnologyCooperation Austria Validation AOACvalidationbodyintheU S NordValandMicroValaretheNordicandEuropeanbodiesProblem lackofacommonvalidationprocedureInhibitsestablishmentofrapidmethods BureauforInternationalResearchandTechnologyCooperation Austria Conclusions ManynewmethodsandtechnologiesarebeingdevelopedIncreasedsamplethroughputandefficiencythroughautomationThemarketforrapidmethodsissmallPenetrationofrapidmethodshasbeenslowLackofacommon