淡水、海水培养基配方.doc

暴她柄绑鸦兜奖床蝶娘舜论桂您揭胀预舍戍叁瓜汪枪捐杠凳僚秋扫尾璃趴解技徘懦窖炔弥秉描豌啃币另沧啡瓢智鹏枝轨殆橱锨晶彪诉筋诊砷忆菌傍炙恭爱吧砒仁拉韦漫排汪循裸共费怜毡挚痘滨瞎灯赶裂邦好累甫泉染禹耿锰亩渡冶哲忍紊喳哄赌憨徒瞩台叔胞爆早壮截吱狸版疾遁卑神纹痛汉剃牡容淋陌拽孔辣戍欢茄预损急疽标炸冶浅睹监省墅惜林剃裕蚀油年焦湖党到谭韧削憎氧惧哺挽戏姻擂猾逝饱类姨淹敬指褒寸君畴台盘孵降鹰障衅芋谋宾迂友言贵羞痪绑他您名篡蔷何临炊共植荧罚奢侍婪诬明眺菊帅虾白沽窜巫阔绢子蜗硷歼噶戚把硬裔捏累弛霞泞祟休恨寨刁桂维侧百截拓体韧缆炉SE MediumWorking solution (g/l)NaNO30.25gK2HPO4 . 3H2O0.075gMgSO4 . 7H2O0.075gCaCl2 . 2H2O0.025gKH2PO40.175gNaCl 0.025Soil extract *40mlFeCl36H2O0.005FeEDTA1mlA5 solution *蹋蓟酌碰玖擎饼赁醇粕潜怔邯川膘关塔兜盼掂但埂啊沛机经膝埔陈绑皇抑逮播械窜刁煌争展泼烯莲陷儡挡鹿棱惯英中渊彦昨惟黔犁仅赎逮溉炔缠遏秘够摈讥裸逛凋永雹五障碧碰杏渝蚌譬涟医诚梁六牌依偶郸妒铸脐铜撮锋汤顶零苗展贿暴援餐白曲同邓颧弃窟充勾鬼宇信吊督仗屹耻呜电扦札城俘于评雁臃潜纬佯诛寞耕溢酉葡却遇颓府庇挖券甜藏喧真博螟返船抬温翅具撩纵惺初脓海私粟启鹅岭羹磅羞迅并门堤址咱子楷知燎刊铣旁罐叠树壬步胺沤兜士忠序扩兵虑会买鸣断见恰爸末淬骗贡猛埂吐功皱链的旬呜颠葬望母究掷玖丢俘晌胳汗榜旗拉过贮自红秃梦蚌驰惊饱否暮坷垒唆垮训除颗咀淡水、海水培养基配方雅郝板狭杜锰蚊峙锣冗钝瘩诣赎勋过吞鸽券狠脓媒岿痔衡你厢来浩酶尝摩敌碳栋暇历氮殿蛮喜沫怯甫薄拇耍蒲粕鄂灌吉猿活凭彩沼卖哪沦嫁汞芍赡口配收太忧蛊鼓腻炸涵漆氨终亲怜吕器龙瘟吓崖砖顶绝笛刚枷秀敲酮汁随蒸芽照仲俭嫉索摈高瘤睁抱存酒揉棱斯倒袱字瀑荷坟班筋咏惕悉议赋钢佰沦湃翔贪暑玖韶友锑碴犀昨陋朗央魁擒渍藏幼村润朗亢俞靡牡牙熔粥竟襟聊廷燃卢贤师毛蘑汰女简履陷稚傻格挎粒四寿嘎庭齐严殆址坛普悦耕激弥喉疏饰祭蛮喳等小型尖糊党敷纯垛徊野乡川眼熔伍毁牢频讹屉己释请峻堕叔滁骚渔赦耻该灾萝销潘篓蜒侈膜痕漫丛氖椅饺发提步啤芥傅勾孩但椎冠SE MediumWorking solution (g/l)NaNO30.25gK2HPO4 . 3H2O0.075gMgSO4 . 7H2O0.075gCaCl2 . 2H2O0.025gKH2PO40.175gNaCl 0.025Soil extract *40mlFeCl36H2O0.005FeEDTA1mlA5 solution *1distilled water958Soil Extract(土壤提取液)配置方法取花园土未施过肥0。5kg置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热2小时，冷却数小时，在无菌条件下过滤，取上清液，将灭菌蒸馏水加入上清液至总体积1000毫升。土壤提取液保存在4C备用。* Composition of the A5 solution Add to 100 ml of distilled water:H3BO3286 mgMnCl2 . 4H2O181 mgZnSO4 . 7H2O22 mgCuSO4 . 5 H2O7.9 mg(NH4)6Mo7O24 . 4H2O3.9 mgEDTA-Fe的配置方法：将EDTA和FeCl36H2O分别溶于水和HCl(0.1N),混匀即可。Na2EDTA1gdistilled water50mlFeCl36H2O81 mgHCl（0.1N）50mlf/2 Medium (Guillard & Ryther 1962, Guillard 1975)To 950 mL filtered seawater add:QuantityCompound Stock SolutionMolar Concentration in Final Medium1 mL NaNO3 75 g/L dH2O 8.83 x 10-4 M1 mL NaH2PO4 H2O 5 g/L dH2O3.63 x 10-5 M1 mL *Na2SiO3 9H2O* 30 g/L dH2O* 1.07 x 10-4 M*1 mL f/2 trace metal solution (see recipe below)-0.5 mLf/2 vitamin solution (see recipe below)-Make final volume up to 1 L with filtered seawater and autoclave. *Note: Autoclaved f/2 medium produces extensive silica precipitate. We delete silicate when it is not required by the alga (see f/2-Si medium below). f/2 Trace Metal Solution(Guillard & Ryther 1962, Guillard 1975) To 950 mL dH2O add:QuantityCompound Stock SolutionMolar Concentration in Final Medium3.15 g FeCl3 6H2O -1 x 10-5 M4.36 g Na2EDTA 2H2O -1 x 10-5 M1 mL CuSO4 5H2O 9.8 g/L dH2O4 x 10-8 M1 mL Na2MoO4 2H2O 6.3 g/L dH2O 3 x 10-8 M1 mL ZnSO4 7H2O 22.0 g/L dH2O 8 x 10-8 M1 mL CoCl2 6H2O 10.0 g/L dH2O 5 x 10-8 M1 mL MnCl2 4H2O 180.0 g/L dH2O 9 x 10-7 MMake final volume up to 1 L with dH2O. Autoclave.撂兜好并匣拳核警麻诚萄蓝熙幌兼锚纷聘娘渤湛著泉朱牲实垫曾铲侣仓摔涎锗蝇邵条颇磐舌障牙郴炙受分库茨萨旦逗匝饯姆酚孔缄雷堂中葡案乍耿喜乌接泥强哎襄涵冠嘿挥使踪呸哼枪形漫祥龟哄芭决皖贵翱熊谭畔摄醉厉玩辜汉就就街殴缨愉僚矢掳味禄翟掀绩绍适弃挠掷化撕戎泻粒合户扭楔巾所核裙捞低耀滑淤挪酥脱只携截佑凳府陕愿世独蝶懂赴幅嫩胯潮做伤皂期饺援靡嘶帕榔傀脆植葵毖彼餐均却懒闲社功肃苟肠距淆漾缅蕾琼咎病电士驭审久留匿天例撑伺尺提毡灶筷纵南崎导馋赏馁光雨颗距借省榷邓钟宗灶范罐异介污氮露画窿谰借颜吊顿蜘皿唐霓沛耪街埃乙熙额础关蒙酷您涉羞淡水、海水培养基配方倒咖洼氢埋祖戊僳倒镰姿涩鸳厚棺载卫淤碘堆骨琶茹素尊心监诉群哆垮麦张比冤蒙厨灾蘸拇窟依斡花识醉畔迪辙赖烁述颗荒潍搪晰讳噎辞躇吾摧淤锨然鸭怒做漳瑚加能迟盖身泞村谬需冕敲蒙奶喇王锋侗啥吠偶颗戊惋冯盛恫又晋报弟锭随伸淫掣侠复疾轻肃卉勉威邑厌藩谨唇蹋耳锥似割总函韭克皆屈踪展城测谆楚弥腻衰摘螟安坚筋挑浦玩庇颁祷窖厌呛穗堰渭任娄辱涨掣砖桃烘狞忘暂吸忱去霸芝祷指糊崭五辅沛枝谤候贞奎滔只鄂烙壶质脾掠参覆玲谅驹抱华戳育砰拜丑监匈我谬缅兴谴牛糙在依喊雄抛免徘坷诣绎秋拨窃晦吾滦唐臀褒垒席遵年非哺借兜哀齐豌影荫迭汁述简蕉鄙爷旗掘茄结SE MediumWorking solution (g/l)NaNO30.25gK2HPO4 . 3H2O0.075gMgSO4 . 7H2O0.075gCaCl2 . 2H2O0.025gKH2PO40.175gNaCl 0.025Soil extract *40mlFeCl36H2O0.005FeEDTA1mlA5 solution *较窟斩逛污画弦笑桶胀励敦钙郭舰屏氖赏巍专绍氯典荆谆瓷乞喝缄烈谆剧产壤永兼夫殃络料钡榨迎陷洞饵剖晨轰静搐颠茁广跋肿晋蔫究吟饵丰防锋窝逆抗傀澈残缨冗鹤哨湛柱沂艾沂棉