athogeniccocci病原性球菌.ppt

1 Pathogeniccocci2 Otherbacteria 1 PathogeniccocciConceptofpathogenicbacteria13minStaphylococcus Esp S aureus 56minStreptococcus Esp S pyogenes 38minEnterococcusspeciesandVRE5minNeisseriameningitidesNeisseriagonorrhoeae13min2 OtherbacteriaPseudomonasaeruginosa10min Highlightsofcontent Uptoeverypathogen thefollowingwillbetalked BiologicalpropertiesPathogenicityImmunityMicrobiologicaldetectionsPreventionandtreatment Pustule 脓疱 byS aureus Pustule 脓疱 byS aureus Paronychia 甲沟炎 byS aureus Sorethroat 咽喉炎 byS pyogenes Untreatederysipelas 丹毒 byS pyogeneswithskinsloughingandnecrosis NecrotizingFasciitis 坏死性筋膜炎 byflesh eatingbacteria S pyogenes anaerobicbacteria etc andinsects bee etc stinging Chestx rayFilm1justafewhoursofrigorsandproductivecough Chestx rayFilm212hourslaterconsolidationintherightupperlobe Lobarpneumonia 大叶性肺炎 byS pneumoniae Meningococcalsepticemia 脑膜炎球菌性败血症 byN meningitidis widespreadecchymoses Gonococcalconjunctivitis 淋菌性结膜炎 byN gonorrhoeae Causativeagentsofaboveclinicaldiseases WhatisPathogeniccocci alsocalled Pyogeniccocci 化脓性球菌 Including Staphylococcus S aureus S epidermidis 金黄色葡萄球菌与表皮葡萄球菌 Streptococcus GroupAStreptococci S pyogenes A群链球菌or化脓性链球菌 Streptococcuspneumoniae 肺炎链球菌 Neisseriagonorrhoeae 淋病奈瑟菌 Neisseriameningitidis 脑膜炎奈瑟菌 Importantcocci Staphylococcusaureus ImportantpyogeniccoccusLeadingpathogenofwoundinfections includingotherstaphylococci Shape Stain Culturespherical elliptical irregulargrape likeclusterGram G Growwellonbasicmedium Biologicalproperties AntigenicstructureStaphylococcalproteinA SPA OnekindofsurfaceproteinincellwallSinglechainpeptideActions Binds bacterium tohost sIgG1 IgG2 IgG4coagglutinationtestCompetesw Fcreceptorsofphagocytesresistsphagocytosis Coagglutinationtestwithinertparticles e g heat killedS aureusorlatexbeads SPAmayresistphagocytosisbycompetivelybindingwithFcreceptorsofphagocytes CapsuleTeichoicacid polysaccharide polysaccharideA S aureuspolysaccharideB S epidermidis Phasesofphagocytosis ImportantPropertiesof3MainSpeciesofStaphylococci Pathogenicity immunity Pathogenicsubstances Coagulase VirulenceProtectionactionsInfectionlocalized Boundcoagulase 结合凝固酶 orclumpingfactor 凝聚因子 plasmatobeclotedorbacteriatocoagulateSlidecoagulasetest classifiedinto toxin toxin makesporesincellmembranes Staphylolysin 葡萄球菌溶素 killsordegranulatesneutrophils macrophages includingtypeA typeBcausesstaphylococcalscaldedskinsyndrome SSSS Exfoliatin 表皮剥夺毒素 Others heat stablenuclease 耐热核酸酶 Clinicaldiseases Invasivediseases Toxin mediateddiseases Pyogenicinfections localinfections systemicinfections foodpoisoning staphylococcalscaldedskinsyndrome SSSS toxicshocksyndrome TSS Folliculitis 毛囊炎 byS aureus BullousImpetigobyS aureus AS aureusinfectionona12 year oldchild scheek Pathogenesisofstaphylococcalinfections Microbiologicaldiagnosis Identificationofpathogenicstaphylococci Coagulase Heat stablenuclease Goldenpigment Hemolyzation Mannitefermentation Prevention treatment Methicillin resistantS aureus MRSA 耐甲氧西林金黄色葡萄球菌 Coagulase negativestaphylococci CNS 凝固酶阴性葡萄球菌 Comparethefeaturesofcommonpyogenicbacteria DiscussthepathogenicfeaturesandidentificationpointsofStaphylococcusaureus Questions Streptococci G spherical elliptical arrangedinchainGrowwellonnutrientmediumw blood Biologicalproperties Shape Stain Culture Antigenicstructure Proteinantigen surfaceAg outsideCantigen type specific includingM T R SinGroupAPolysaccharidantigen CAg group specificNucleoprotein PAg non specific Classification Pathogenicity GroupAStreptococci S pyogenes Pathogenicsubstances StreptolysinS SLS 链球菌溶素S lowMWglycopeptide oxygen stable causes hemolysisonBloodAgarplate non antigenic StreptolysinO SLO 链球菌溶素O proteinw SHgroup oxygen labile destroysmanykindsofcells anti SLOtest ASOtest helpfulfordiagnosis Streptolysin codedbylysogenicbacteriophage proteinsincludingserotypeA B C causesscarletfever 猩红热 onekindofsuperantigens alsocausesTSS Streptococcalpyrogenicexotoxin SPE 链球菌致热外毒素 Streptokinase SK 链激酶 alsocalledasstreptococcalfibrinolysin 链球菌溶纤维蛋白酶 Streptodornase SD 链道酶 hydrolyzesDNA Hyaluronidase 透明质酸酶 alsocalledasspreadingfactor 扩散因子 hydrolyzeshyaluronicacidinconnectivetissue helpfulforspreadingofbacteria Clinicaldiseases Pyogenicinfections Toxin mediateddiseases Allergicdiseases latecomplications scarletfever 猩红热 Streptococcaltoxin mediatedTSS rheumaticfever 风湿热 acuteglomeruloniphritis 急性肾小球肾炎 Erysipelas 丹毒 byS pyogenes Cellulitis 蜂窝组织炎 byS pyogenes Honey crust lesiontypicalofS pyogenesimpetigo PathogenesisofS pyogenesinfections Samples Pus Blood Nasopharynxswab Isolationw bloodagarplate distinguishingfromS pneumoniae Smear microscopy G streptococci distinguishingfromstaphylococciOtherstreptococci hemolysis Assist diagnosisforrheumaticfever hemolysis ASOtest Microbiologicaldiagnosis S pneumoniaeGroupBstreptococciGroupDstreptococciViridans hemolytic streptococciStreptococcussuis 猪链球菌 Enterococcusspecies 肠球菌 VRE Otherstreptococci EnterococcusspeciesandVRE Enterococcusspp 肠球菌 PreviouslyclassifiedasGroupDstreptococciIn1984 namedasgenusEnterococcusGram cocciinpairsorshortchainsMostarefacultativeanaerobesComplexnutritionalrequirementsGroupDspecificteichoicacidantigen Enterococcusspp viewedbydirectfluorescentantibody FA reaction Enterococcusspp Commonlyfoundintheintestinesofnormalhealthyindividuals humansandanimals 2importanthumandiseases relatedspp E faecalis 粪肠球菌 andE faecium 屎肠球菌 CauseUrinarytractinfectionsBacteremia septicaemiaSubacuteendocarditisWoundinfectionsFoodbornediseasesMeningitisNosocomialinfections Oneofimportantlife threateningnosocomialpathogensVancomycinResistantEnterococci VRE 耐万古霉素肠球菌 Since1986 VREinfectionshasbecomeagrowingproblem 1 DiscussthepathogenicfeaturesofStreptococcuspyogenes 2 Discussidentificationpointsbetweenhemolyticstreptococciandotherpyogenicbacteria Questions Neisseriagonorrhoeae 淋病奈瑟菌 Biologicalproperties Shapeandstain G diplococciinoronPMNsonpussmear Noendospore flagellum w capsule pili Culture Obligateaerobes need5 CO2whileprimaryisolation Highnutritionalrequirement ChocolateBloodAgarPlate Antigenicstructureandclassification Pilusproteinantigens Outermembraneproteinantigens PI PII PIII LOS lipooligosaccharide 脂寡糖 LPS like N gonorrhoeaecoloniesonChocolateBloodAgarPlate Pathogenesis Virulentfactors Pili Outermembraneproteins LOS IgA1protease Others Clinicalfindings Gonorrhea Gonococcalconjunctivitis Gonorrheasymptoms ClinicalmanifestationsofN gonorrhoeaeinfection Immunity HumoralimmunitymoreeffectiveNopersistentimmuneresponsesInfectiontrendto chronic repeatedoccurrence Microbiologicaldetections Nucleicacidsdetection soon Samples Pussecretafromgenito urinarytracts eyes others Isolation OxidasetestSugarfermentationtestImmunofluorescenceassay Smear microscopy G diplococciinoronPMNs Colonies microscopicfindings Biochemicalreactions serologictests Othertests Prevention treatment TakepreventionsasmainmeasureChoosepenicillinasNo 1drug takecareofproblemsofdrugs resistantbacterialstains 1 WhatarethemaindifferencesbetweenNeisseriameningitidesandNeisseriagonorrhoeae Question Pseudomonasaeruginosa 铜绿假单胞菌 P190 Shape Stain CultureG bacilisingleterminalflagellumappearscapsuleinsome