撰写临床SCI论文实例剖析10Oct.ppt

1985 1990 同济医科大学 华中科技大 附属协和医院1990 1995 爱尔兰都柏林大学三一学院St JamesHospital1995 2000 澳大利亚悉尼大学NepeanHospital2000 2006 香港大学玛丽医院2006 2014 诺华制药美国 夏华向HarryHua XiangXia 2012 至今 JournalofClinicalandTranslationalHepatology主编2014 至今 北京首都医科大学附属友谊医院 青岛大学附属青岛市市立医院 广东药学院附属第一医院客座教授 1 1988年 2015年发表论文 中文著作1本中文论文23篇论著8篇综述 编者按15篇 英文论文156篇论著125篇第一 并列第一 作者60篇通讯作者17篇综述 编者按23篇第一作者18篇信稿8篇第一作者1篇著作章节10节第一作者6节 2 范学工 夏华向 幽门螺杆菌感染 基础和临床 第1版 湖南 湖南科学技术出版社1997 第一次印刷 1998 第二次印刷 3 医学论文撰写基础技能及实例剖析夏华向美捷登生物科技有限公司主编青岛大学青岛市立医院客座教授青岛大学青岛市立医院SCI论文撰写与国家自然科学基金申请培训班2015年10月10日青岛 4 撰写与发表论文的五个基本层次及要素 一 创意 新颖 创新 重要性Creativity Novelty InnovationandSignificance Implications二 生物医学知识 学术标准及专业指南BiomedicalKnowledge AcademicStandardsandSpecialtyGuidelines更新的生物医学知识 理论和技术更新的学术标准及本专业进展 指南 共识 诊断标准 医护标准Guidelines Consensus StandardsofCare三 撰写科技论文规范与技巧GuidelinesandTechniquesforScientificWriting撰写论文的技巧 全文 部分 段落的结构布局以及句子 短语以及单词选择及运用 5 撰写与发表论文的五个基本层次及要素 四 语言 句法 语法及拼写Syntax GrammarandSpelling语句 结构是否完整 表达是否清晰 明了 具一致性 逻辑性动词时态 过去 现在 现在完成 将来 虚拟 等拼写五 格式 文体Formatsandstyles一般要求各杂志具体要求 6 实例简介 研究背景 设计及结果 5min 论文讲解及技能训练Q A 10min 讲解时间安排 第一层次讲解 创意 新颖 创新 重要性 5min 第二到第五层次 前言 15min 方法 15min 结果及图表 15min 讨论 20min 致谢 5min 参考文献 5min 文稿格式 5min 文题及短标题 10min 署名 5min 摘要 10min 回复信 5min 7 原文题英文 Levofloxacin basedTripleandSequentialTherapyforHelicobacterpyloriEradication aMulticenter RandomizedandControlledTrialinChinesePopulation 中文 基于左氧氟沙星的三联及序贯疗法根除幽门螺杆菌 一项在中国人群的多中心随机对照研究为什么选择MJ5206 研究类型 典型临床试验研究ClinicalTrial胃肠感染性疾病 指征 幽门螺杆菌感染H pyloriinfection通讯作者张国新教授授权 实例简介 MJ5206 8 语言中文为主 必要时 英文原文标记及评论红色背景及评论 生物医学知识 学术标准及专业指南问题红色字体及评论 撰写科技论文规范与技巧问题黄色背景 简单语言及格式问题绿色背景 恰当或正确的描述同类问题 一般只标记一次技能训练论文中蓝色背景相应部分有关的写作知识及例句及有奖问答互动交流 提问及讨论鼓励就正在剖析内容提问 解答 讨论无绝对正确答案 讲解方式 幽门螺杆菌HelicobacterpyloriUnidentifiedcurvedbacilli Campylobacterpyloridis Campylobacterpylori幽门螺杆菌感染率Upto50 indevelopedcountriesUpto90 indevelopingcountries about60 inChinesepopulation幽门螺杆菌感染与胃肠疾病急慢性胃炎acuteandchronicgastritis消化性溃疡病pepticulcerdisease胃癌gastriccancer 实例论文的课题研究背景 10 NobelPrizeinMedicinein2005 BarryJ MarshallandJ RobinWarrenfortheirdiscoveryof thebacteriumHelicobacterpylorianditsroleinGastritisandPepticUlcerDisease AnnouncementonOctober3 2005 11 临床诊断Invasive Biopsy basedmethods culture pathologyandrapidureasetestNon invasive Serology 13C or14C ureabreathtest治疗First line TripleorquadrupletherapySecond line quadruple orsequentialtherapy 实例论文的课题研究背景 12 本项研究背景及目的Decreasingefficacyofcurrentlyrecommendedfirst linetripleandquadrupedtherapieswithincreasingdrug resistanceratesNohead toheadcomparisonbetweenlevofloxacin containingsequentialtherapyandstandardsequentialtherapyasthefirst linetreatmentTocompareefficacyandadverseeventsoflevofloxacin containingtripleandsequentialtherapiesvs standardsequentialtherapy 实例论文简介 13 研究设计及方法 实例论文简介 14 结果参考文献 MalfertheinerP MegraudF O MorainC etal CurrentconceptsinthemanagementofHelicobacterpyloriinfection theMaastrichtIIIConsensusReport Gut 2007Jun 56 6 772 81 MalfertheinerP MegraudF O MorainCA etal ManagementofHelicobacterpyloriinfection theMaastrichtIV FlorenceConsensusReport Gut 2012May 61 5 646 64 刘文忠 谢勇 成虹等 第四次全国幽门螺杆菌感染处理共识报告 胃肠病学 2012 17 10 618 25 实例论文简介 15 WhatisunmetneedsNoeffectivetreatmentconsistentlyachievingeradicationrateof 85 WhatisunknownorunclearLevofloxacinsequentialtherapyasfirstlinetreatmentWhataretheclinicalsignificance implicationsEradicationofH pyloriinfectionWhatistobeverifiedoraddedtothescientificliteratureNoveltherapy Levofloxacinsequentialtherapy 第一层次分析 创意 新颖 创新 重要性 16 SelectingandFinalizingDataResultsMaterialsandMethodsIntroductionDiscussionTitleReferencesAbstractOtherSections GeneralOrderofWriting 17 ThetopictobecoveredandtherationaleIntroductionofthemajorplayersKnownandespeciallyunknownorunresolvedissuesThehypothesisleadingtothestudyTheaim s ofthestudy Introduction Whatandhowtowrite 18 Introduction Howtowrite BackgroundoftherelevantresearchareaCurrentlyknownknowledgeandinformationUnknown unanswered unmetissuesSpecificquestion s Aimsofthestudy seekanswer s forthespecificquestion s 19 Knownandunknown Questions TipsforIntroduction 20 DONOToverlyorpoorlystatetheresearchareaDONOTdiscusstheresultsinIntroductionsectionProperlydefinetheabbreviationsCiterelevant updatedreferencesStatethehypothesisandstudyaimsdirectly Analysis Introduction MJ5206 Levofloxacin basedTripleandSequentialTherapyforHelicobacterpyloriEradication aMulticenter RandomizedandControlledTrialinChinesePopulation TrainingforIntroduction 22 斜体应用 生物 动物 植物 微生物等 种 Species 动 Ailuropodamelanoleucavs Ailuropoda大猫熊属 植 Astragalusmongholicusvs Astragalus黄芪属 微 Helicobacterpylorivs Helicobacter螺 旋 杆菌属核酸内切酶EcoRI EcoRII BamHI HindIII TapI NotI etc 23 斜体应用 拉丁词或短语etc etcetera etal etaliioretalibi i e idest ineffect e g exempligratia examplegiven vs versus adlibitum adhoc posthoc invivo invitro adinterim prn prorenata viceversa perse statusquo enmasse基因 mRNA bcl 2gene bcl 2mRNAvs Bcl 2protein统计参数U test t test P orp value 24 如某相关专业术语在文中出现两次或以上 就应在第一次出现时定义 Definition 缩写 否则 不用缩写 一旦定义 必须在文中持续使用 Consistentuse 摘要和正文均需定义无需定义的缩写 DNA RNA H2O 等在第一次写出Helicobacterpylori全文后 可直接持续用H pylori 缩写用法 25 Exercise1 A HelicobacterpyloriisaGram negativebacterium HelicobacterpyloriinfectionisamajorcauseofgastritisandPUD pepticulcerdisease B Helicobacterpylori H pylori isaGram negativebacterium H pyloriinfectionisamajorcauseofgastritisandpepticulcerdisease PUD C HelicobacterpyloriisaGram negativebacterium H pyloriinfectionisamajorcauseofgastritisandpepticulcerdisease PUD 26 Exercise1 A HelicobacterpyloriisaGram negativebacterium HelicobacterpyloriinfectionisamajorcauseofgastritisandPUD pepticulcerdisease B Helicobacterpylori H pylori isaGram negativebacterium H pyloriinfectionisamajorcauseofgastritisandpepticulcerdisease PUD C HelicobacterpyloriisaGram negativebacterium H pyloriinfectionisamajorcauseofgastritisandpepticulcerdisease PUD 27 中文字体 SimSun新宋体Size10 5Symbols degreeCelsius Fl fi Onecharacter 28 权威标准 指南或共识 MELD ModelforEnd StageLiverDiseaseRomeIIICriteria NewStandardforFunctionalGastrointestinalDisordersPASI75 A75 reductioninthePsoriasisAreaandSeverityIndex PASI scoreIADPSGcriteria TheInternationalAssociationofDiabetesandPregnancyStudyGroups IADPSG newcriteriafordiagnosisandclassificationofdiabetesinpregnancybasedontheassociationofmaternalglycemiawithperinataloutcomesreportedintheHyperglycemiaandAdversePregnancyOutcome HAPO study WOMAC TheWesternOntarioandMcMasterUniversitiesArthritisIndexMaastrichtConsensusIV CurrentconceptsinthemanagementofHelicobacterpyloriinfectionPrimaryoutcomesfortransplantation graftloss censoredfordeath acuterejection andsteroidresistantrejection 29 However although though despite inspiteof IthasbeengenerallyacceptedthatH pyloriinfectioniscausallyassociatedwiththedevelopmentofgastriccarcinoma However onlyasmallproportionofpatientswithH pyloriinfectiondevelopgastriccarcinoma AlthoughonlyasmallproportionofpatientswithH pyloriinfectiondevelopgastriccarcinoma theimpactofH pyloriinfectioncannotbeignored H pyloricansurviveinthehumanstomachalthoughthestomachsecretsgastricacid H pyloricansurviveinthehumanstomachdespite inspiteof gastricacidsecretedinthestomach 30 And aswellas or Don tstartasentencewith And Aswellas or Or Use and whenAandBareimportantatthesameorsimilarlevel BothH pyloriinfectionandhepatitisBvirusinfectioninbloodweredetected Use aswellas whenBislessimportantthanA H pyloriinfection aswellashepatitisBvirusinfection inbloodwasdetected Use or whenAandBarenotoverlappedH pyloriinfectionorhepatitisBvirusinfectioninbloodwasdetected 31 Exercise2 A Clinicalefficacyaswellasadverseeffectsofalevofloxacin containingtripletherapy andamodifiedlevofloxacin containingsequentialtherapywereinvestigated B Bothclinicalefficacyandadverseeffectsofalevofloxacin containingtripletherapy andamodifiedlevofloxacin containingsequentialtherapywasinvestigated C Clinicalefficacyaswellasadverseeffectsofalevofloxacin containingtripletherapy andamodifiedlevofloxacin containingsequentialtherapywasinvestigated Exercise2 A Clinicalefficacyaswellasadverseeffectsofalevofloxacin containingtripletherapy andamodifiedlevofloxacin containingsequentialtherapywereinvestigated B Bothclinicalefficacyandadverseeffectsofalevofloxacin containingtripletherapy andamodifiedlevofloxacin containingsequentialtherapywasinvestigated C Clinicalefficacyaswellasadverseeffectsofalevofloxacin containingtripletherapy andamodifiedlevofloxacin containingsequentialtherapywasinvestigated 33 可能性Can could may might belikelyto probably possibly perhaps maybe Exercise3Discussion OrderthepossibilityA H pyloricansurviveinthemousestomach B H pylorimaysurviveinthemousestomach C H pyloricouldsurviveinthemousestomach D H pylorimightsurviveinthemousestomach E H pyloriwouldsurviveinthemousestomach F H pyloriismore mostlikelytosurviveinthemousestomach G H pyloriislikelytosurviveinthemousestomach H H pyloriisless leastlikelyorunlikelytosurviveinthemousestomach I H pyloriisprobablyassociatedwithheartdisease J H pyloriispossiblyassociatedwithheartdisease K PerhapsH pyloriisassociatedwithheartdisease L MaybeH pyloriisassociatedwithheartdisease 34 根据basedonvs onthebasisof事实上actually infactvs asamatteroffact TherapidureasetestiscreatedbasedonthefactthatH pyloriproducesalargeamountofurease TherapidureasetestiscreatedonthebasisofthefactthatH pyloriproducesalargeamountofurease Exercise4 35 根据basedonvs onthebasisof事实上actually infactvs asamatteroffact Asamatteroffact H pyloriproducesalargeamountofurease Actually Infact H pyloriproducesalargeamountofurease Exercise5 36 Also too Don tuse too inascientificarticlewhenitmeans also Also isnormallynotusedintheendofthesentence 37 Unknown unclear uncertain controversial tobeelucidated tobeconfirmed lacking WhetherH pylorieradicationcandecreasetheriskofcancerremainsunknown WhetherHelicobacterpylorieradicationaltersgastricmucosalphospholipidcontentsandtheirfattyacidcompositionremainsunclear WhetherHelicobacterpyloriinfectionisassociatedwithdiabetesmellitusanditsassociatedgastrointestinalsymptomsremainsuncertain WhetherHelicobacterpyloriinfectionisassociatedwithdiabetesmellitusanditsassociatedgastrointestinalsymptomsremainscontroversial HowHelicobacterpyloriinfectionleadstothedevelopmentofgastriccarcinomahasnotbeenfullyelucidated WhetherthistripleregimenachievesthesameclinicalefficacyneedstobeconfirmedinChinesepatients 38 Research investigation study ies experiment s report s Marshall theNobellaureate continueshisresearchonH pyloriandrunstheH pyloriResearchLaboratoryattheUniversityofWesternAustralia FurtherinvestigationisneededtoevaluatetheeffectsofH pyloriinfectionanditseradicationamongdifferentagegroupsandinrelationtoobesity Therefore theaimofthepresentstudywastodeterminetheclinicalefficacyofalevofloxacin containingtripletherapy andamodifiedlevofloxacin containingsequentialtherapy Theinvitroexperimentwasperformedintriplicate Wangetal presentedacasereport 39 Exercise6 InaresearchbyWangetal drugAwasmoreeffectivethandrugBinthetreatmentofdiabetesmellitus InaninvestigationbyWangetal drugAwasmoreeffectivethandrugBinthetreatmentofdiabetesmellitus InastudybyWangetal drugAwasmoreeffectivethandrugBinthetreatmentofdiabetesmellitus Exercise6 A InaresearchbyWangetal drugAwasmoreeffectivethandrugBinthetreatmentofdiabetesmellitus B InaninvestigationbyWangetal drugAwasmoreeffectivethandrugBinthetreatmentofdiabetesmellitus C InastudybyWangetal drugAwasmoreeffectivethandrugBinthetreatmentofdiabetesmellitus 41 Explore investigate determine evaluate assess identify compare validate verify confirm Exercise7Discussion Whichofthefollowingstudieswouldgeneratehigh qualityresults Theaimofthepresentstudywastoexplore investigate determine theassociationbetweenH pyloriinfectionandesophagealsquamouscellcarcinoma Theaimofthepresentstudywastoevaluate assess determine theefficacyandsafetyofanoveltripleregimeninadultswithH pyloriinfection Theaimofthepresentstudywastoidentify determine theriskfactorsinHpylori inducedgastriccarcinogenesis TheaimofthepresentstudywastocomparetheefficacyandsafetyofanoveltripletherapywithastandardtripletherapyinadultswithH pyloriinfection Theaimofthepresentstudywastovalidate verify confirmtheefficacyandsafetyofanoveltripletherapyinChineseadultswithH pyloriinfection 42 MaterialsandMethods SpecificSufficientdetailsthatallowotherstorepeattheexperimentsConsistentwiththeResultssection 43 Tips TipsforMaterialsandMethods AllmethodsusedtogeneratethedatapresentedinresultsDONOTshowanyresultsinthissectionCitationsforprotocolsusedpreviouslyPatientsinclusionandexclusioncriteriaStrainsandagesofanimals dosesused treatmentroutesanddurations andotherdetailsthatwouldallowthereadertorepeattheexperimentsSamplesizecalculationforclinicalstudies especiallyforclinicaltrialsMethodsusedtoanalyzedataEthicissues 44 Analysis Materials Methods MJ5206 Levofloxacin basedTripleandSequentialTherapyforHelicobacterpyloriEradication aMulticenter RandomizedandControlledTrialinChinesePopulation Tips TrainingforMaterialsandMethods 46 Yourmanuscriptwasreceived however theeditorialboardfindsthat whileitcontainsinterestingresults itcannotbeacceptedforthepublicationinNatureduetoalargenumberofhigh qualitymanuscriptssubmittedtothejournal Exercise8 Whatisthedifference 接收和接受不分Receivevs accept 47 Consentform ProspectiveclinicalstudiesWitteninformedJustificationrequiredifonlyforverbalinformedconsentformEthicsapprovalforextraordinarycircumstances 48 Ethicconsiderations TheInstitutionalReviewBoard TheEthicsCommittee EthicalReviewBoardformallydesignatedtoapprove monitor andreviewbiomedicalandbehavioralresearchinvolvinghumansAll prospectiveandretrospective clinicalstudiesusinghumanmaterialsneedapproval AnimalEthicsCommittee TheLaboratoryAnimalEthicsCommittee CommitteeforEthicsinAnimalExperimentation CEAE Allanimalexperimentsneedapproval 49 Exercise9 A Eachpatientprovidedwritteninformedconsent andthetrialwasapprovedbyalocalethicscommittee B Eachpatientprovidedverbalconsent andthetrialwasapprovedbytheInstitutionalReviewBoardofXXXUniversity C Eachpatientprovidedwritteninformedconsent andthetrialwasapprovedbytheInstitutionalReviewBoardofXXXUniversity Exercise9 A Eachpatientprovidedwritteninformedconsent andthetrialwasapprovedbyalocalethicscommittee B Eachpatientprovidedverbalconsent andthetrialwasapprovedbytheInstitutionalReviewBoardofXXXUniversity C Eachpatientprovidedwritteninformedconsent andthetrialwasapprovedbytheInstitutionalReviewBoardofXXXUniversity 51 It s you re won t can t couldn t haven t don t didn t etc ContractionsarenotusedinformalwritingAvoidusingcontractionsinascientificarticleA ThepresentstudyshowedthatH pyloriinfectionwasn tassociatedwithdiabetesmellitus B ThepresentstudyshowedthatH pyloriinfectionwasnotassociatedwithdiabetesmellitus Exercise10 52 原因Because becauseof dueto as since for thanksto owingto Because becauseof dueto arecommonlyusedH pyloricansurviveinthehumangastricmucosabecauseitproducesalargeamountofurease H pyloricansurviveinthehumangastricmucosabecauseof duetoitspotentureaseactivity as and since arealsocommonlyused butweakerinexpression H pyloricansurviveinthehumangastricmucosaas sinceitproducesalargeamountofurease thanksto and owingto areusedforspecialcircumstancesThanksto owingtoitspotentureaseactivity H pyloricansurviveinthehumangastricmucosa Avoidusing for 53 Commonlyusedstatisticalanalysismethods 54 多余用词 ResearchresultsTheresearchresultsofthepresentstudyareconsistentwithpreviousobservations TheresultsofthepresentstudyareconsistentwithpreviousobservationsNo10 JiefangRoadNo10 JiefangRoad Wuhan Hubei China3003310JiefangRoad Wuhan Hubei China30033 55 Results Whatandhowtowrite ThemostinterestingresultsAccurateandconcisedescriptionsofyourresultsIllustrationsofvarioustypes e g figuresandtables PropersubheadingsConsistentwiththeMethodssectionNorepetitionofMethodsNoattempttointerprettheresults 56 Tips TipsforResults Showdetailedstatistics Pvalue oddsratio confidentialinterval etc withresultsDONOTdiscussMethodsandMaterialsDONOTincludetitlesinfigures Thisinformationshouldbeinthefigurelegends DONOTshowtoomanyfiguresandtables Wellwrittenmanuscriptscontainonlyafewimportant clearfigures DONOTcitereferences 57 Analysis Results Analysis Results MJ5206 Levofloxacin basedTripleandSequentialTherapyforHelicobacterpyloriEradication aMulticenter RandomizedandControlledTrialinChinesePopulation Tips TrainingforResults 59 数字Tenor10 数字在句首 所有全拼 数字在句中 小于10的全拼 AMA例外 时间 单位例外 TwentypatientswithH pyloriinfectionand20age andsex matchinghealthyvolunteerswereselected Atotalof366 week oldfemaleC57BL 6micewereequallydividedintosixgroups Eligiblepatientsweretreatedwith20mgesomeprazoletwicedaily 1gamoxicillintwicedaily 500mglevofloxacinoncedaily 60 Exercise11 A 209patientswererecruitedinthestudy 9patientswereexcluded Thus twohundredpatientsweretreatedwithomeprazole20mgb i dforfourweeks B Atotalof209patientswererecruitedinthestudy ninepatientswereexcluded Thus 200patientsweretreatedwithomeprazole20mgb i dfor4weeks C Atotalof209patientswererecruitedinthestudy ninepatientswereexcluded Thus 200patientsweretreatedwithomeprazole20mgb i dforfourweeks 61 Exercise11 A 209patientswererecruitedinthestudy 9patientswereexcluded Thus twohundredpatientsweretreatedwithomeprazole20mgb i dforfourweeks B Atotalof209patientswererecruitedinthestudy ninepatientswereexcluded Thus 200patientsweretreatedwithomeprazole20mgb i dfor4weeks C Atotalof209patientswererecruitedinthestudy ninepatientswereexcluded Thus 200patientsweretreatedwithomeprazole20mgb i dforfourweeks 62 显著Significant obvious remarkable noticeable evident Therewasasignificantdifferenceinthedetectionrateofearlycarcinomabetweenthetwogroups 13 7 vs 7 3 OR 2 089 95 CI 1 002 3 783 2 XXXX P 0 010 Table2 GroupAachievedasignificantlyhigherdetectionrateofearlycarcinomathangroupB 13 7 vs 7 3 OR 2 089 95 CI 1 002 3 783 2 XXXX P 0 010 Table2 ThedetectionrateofearlycarcinomawassignificantlyhigherinGroupAthaningroupB 13 7 vs 7 3 OR 2 089 95 CI 1 002 3 783 2 XXXX P 0 010 Table2 ThedetectionrateofearlycarcinomainGroupAwassignificantlyhigherthanthatingroupB 13 7 vs 7 3 OR 2 089 95 CI 1 002 3 783 2 XXXX P 0 010 Table2 63 Obviously H pyloriinfectionisnottheonlyfactorthatcontributestothed