电泳实验报告

u fe oh 3 nacl 1 6 fe cl3 fe oh 3 2 u fe oh 3 5 0cm u nacl u 3 u u fe oh 3 nacl 51 1 2 1 ph 2 0 f 0 1 2 0 3 f1 f1 qe 1 q e fe oh 3 f 2 stokes f2 4 r 2 r f1 f2 1 2 qe 3 4 r q 4 r r e 5 4 4 6 e e c g s si r m m s 1 pa s e v m 1 4 9 109 7 e 213 2 250ml 1 fec13 10 kcl 0 02 mol l 1 1 2 fec13 fe oh 3 fecl3 3h2 ofe oh 3 3hcl fe oh 3 hcl feocl 2h2o feocl feo cl feo fe oh 3 m y fe o y z cl z z cl 3 fe oh 3 kcl kcl pt k cl 180v a b c u v l m e u l 1 1v m m s m s e m 1 20 80 37 f 4 e 20 0 001pa s 1 fe oh 3 2 kcl fe oh 3 q e f1 eq f2 k ru u f1 f2 qe k ru 1 q r 2 2 1 u e k 3 t s l e l u s t 4 3 s 4 l t s t fe oh 3 kcl 1 2 kcl 3 fe oh 3 1 kcl 2 40ml fe oh 3 3 fe oh 3 4 60v 5 6 4 7 7 8 28 3 101 87 kpa 27 7 100 76 kpa 60 00 v l cm 32 90cm 0 000894pa s 78 36 u 60v l 32 90cm s 4 l t k 4 l 0 0531 cm min 1 78 36 f m 0 8904mpa s l 32 90 cm 60v 4 172 10 6 v fe oh 3 4 172 10 6 v fe oh 3 1 2 3 4 fe oh 3 1 2011 11 03 09 43 56 0 1 2 3 rna ph2 2 5 1 2 3 rna ph2 2 5 ph 8 5 1 dna dna dna 2 dna dna dna dna dna dna dna dna dna dna dna 3 dna dna 2kb dna 5v cm 4 dna dna 10 dna ethidium bromide eb 1 dna 254nm eb sybergreen dna rna rna northern rna dna rna 1 50 tae 1000ml 242g tris 57 1ml 18 6g edta 2 eb 100ml 1g 3 dna 0 25 0 25 50 w v 4 rna 0 25 0 25 1mm edta ph8 0 50 w v depc 5 5 0 1m mops ph7 0 40mm 5mm edta ph8 0 depc dna dna kb 0 3 60 5 0 6 20 1 0 7 10 0 8 0 9 7 0 5 1 2 6 0 4 1 5 4 0 2 2 0 3 0 1 1 1x 2 1mm 50 0 5 eb eb 0 5 g ml eb 15 1x 3 dna 1x 10 l 4 dna 5v cm 0 5 1cm 5 254nm rna 23 ml 0 336g 20ml depc 60 c 5ml 5x 5 5ml 30 rna 1 l rna 5 0 5 l 0 7 l 2 l 65oc 15min 1 l 0 2 l eb 1 3 30 2 3 1 10 min 5v cm 4 3 4v cm 5 254nm 1 eb eb eb 0 5mg ml 0 2 5 50 0 12 0 5mol l 1 1mol l eb 1 200 2 eb dna dna dna 0 5 g ml eb 3 rna rna 28s rrna 18s rrna mrna rna 28s 18s rrna 4 5kb 1 9kb 28s 18s 1 5 2 5 1 rna 0 5 3 0kb 28s 18s 1 1 rna 28s 18s rrna 1 2 eb dna m 1kb dna ladder 1 dna 2 dna 3 dna ff 2 2 0 5 tbf 300bp dna ff 4kb dna 0 5 1 4 ph dna dna dna dna 1 2 1 1mol l tris cl ph8 0 12 11g tris 80 ml ddh20 4 2 ml ph 8 0 100 ml 2 0 5 mol l edta ph8 0 18 61g na2edta 2h2o 80 ml ddh2o naoh ph 8 0 2 g 100 ml 3 100 mmol l tris cl ph8 0 20 mmol ledta 500 mmol l nacl 1 5 sds 4 80 4 16 5 6 loading buffer 0 15 0 15 ff 5 mmol l edta 50 1 15ml 5ml 60 2 1 2g 60 20 min 3 5000 rpm