MGMT与肿瘤的预见性化疗

O6 DNA O6 methylguanine DNA transferase MGMT DNA O6 DNA AGAT alkylating agents DNA DNA 6 MGMT Mer Mer Mer Mer MGMT Mer O6 BG Mer M GMT 1 MGMT 1 4 DNA DNA DNA 12 DNA O6 DNA O6 DNA DNA 1 3 MGMT MGMT MGMT MGMT MGMT MGMT MGMT MGMT MGMT ACNU MGMT MGMT MG MT 1987 14 20 MGMT MGMT MGMT MGMT MG MT MGMT empirenews page 20 4 4 2 2 2 1 HeLa S3 Mer HeLa MR Mer MGMT ACNU BCNU 20 3 SHG 44 BGC 823 BEL 7402 SMMC 7721 20 MGMT 2 10 Scudiero MGMT 20 19 93 4 Ikenaga 15 6 40 5 MG MT 20 SHG 44 HeLa MR MGMT BGC 823 BEL 7402 SMMC 7721 HeLa S3 10 D1 0 D10 MGMT MGMT MGMT 1 MGMT MGMT Watatani MGMT MNNG MGMT MGMT 6 1 107MGMT HeLa MR SHG 44 MGMT HeLa S3 SMMC 7721 25 g ACNU 1 4 ACNU 50 g 2 HeLa MR SHG 44 1 ACNU MGMT HeLa S3 SMMC 7721 2 MGMT ACNU MGMT 1 MGMT ACNU 1 HeLa MR HeLa S3 ACNU 0 2 HeLa MR HeLa S3 ACNU 25 g g 4 9 HeLa MR HeLa MR ACNU 50 g g 2 2 ACNU MGMT MGMT empirenews page MGMT MGMT DTIC TMZ 7 MGMT SCE MGMT MGMT 8 MGMT 9 MGMT MGMT MGMT 74 MGMT 9 5 24 14 2 MGMT MGMT 74 6 MGMT 3 1 8 1 Isowa 21 MGMT 9 5 10 Frosina 27 MGMT 7 4 11 MGMT MGMT MGMT MGMT 1 2 3 MGMT MGMT cDNA neo pSV2MGMT neo MGMT HeLa MR MGMT cDNA Mer MGMT cDNA 5 4 MGMT MGMT mRNA ACNU MNNG MGMT MGMT mRNA MGMT 2 MGMT mRNA MGMT MG MT MGMT MGMT 3 M er HeLa S3 SMMC 7721 Cc801 3 MGMT ACNU BCNU Streptozotoc in STZ Mer 3 Mer 37 1h MGMT STZ 1 5mmol L HeLa S3 MGMT 1 10pmol mg 0 59pmol mg STZ 1 0mmol L SMMC 7721 MGMT 0 77 0 39 STZ 0 5mmol L Cc801 MGMT 0 39 0 14 3 MGMT ACNU D10 HeLa S3 114 g ml 68 g ml SMMC 7721 77 g ml 47 g ml Cc801 39 g ml 23 g ml STZ 3 0mmol L 2 0mmol L 1 0mmol L HeLa S3 SMMC 7721 Cc801 MGMT 0 01pmol mg Mer HeLa MR 3 Mer ACNU D10 HeLa MR D10 10 g ml 3 Mer ACNU STZ 3 0mmol L 2 0mmol L 1 0mmol L HeLa S3 SMMC 7721 Cc801 BCNU D10 HeLa MR BCNU 10 0 g g STZ 25 g g ACNU ACNU HeLa S3 Mer STZ ACNU O6 O6 benzylguanine O6 BG Mer HeLa S3 SMMC 7721 Cc801 MGMT Mer empirenews page MGMT STZ ACNU 8 1 2 CT ACNU 3 4 12 5 ACNU 6 6 WBC 4 109 L 100 109 L STZ ACNU STZ 2g m2 1h ACNU 2 3mg kg 12 CT 8 ACNU 1 CR 2 PR 50 4 NC 50 1 PD 25 CR PR 37 5 3 8 STZ ACNU STZ ACNU MGMT MGMT MGMT 3 MGMT MGMT APAAP MGMT IgG Fab Fab APAAP APAAP MGMT 3 1 MGMT DNA star MGMT 3 3 8 20 KLH 3 2 MGMT 1 MGMT 14 Fmoc HBTU HOBT Rink MGMT 14 2 keyhole limpet hemocyanin KLH MBS KLH 3 3 6 8 BALB c ELISA 3 4 MGMT 1 2 SP2 0 HAT 3 MGMT 3 5 empirenews page 3 6 MGMT 1 MGMT 7 10 2 MGMT 33 3 MGMT Pharmacia Biotech AKTA FPLC HiTrap q Protein G Sepharose 3 7 MGMT IgG MGMT IgG1 80 98 MGMT 1 40 3 8 MGMT 1 1 MGMT MGMT MGMT ACNU MGMT STZ MGMT 16 1 2 3 MGMT MGMT MGMT 4 MGMT 4 1 C pG CpG island 5 20 0bp GC CpG MGMT CpG MGMT MGMT CpG 45 DTIC TMZ MSP methylation specific PCR 19 DTIC TMZ 9 9 19 47 MGMT CpG 9 7 7 9 78 MGMT 26 DTIC TMZ 26 5 5 26 19 MGMT CpG 5 1 1 5 20 MGMT MGMT CpG DTIC TMZ MS P empirenews page 4 2 Temozolomide Temodar 3 4 3 4 5 1 d 1 2 3 5 8 Schering plough 1999 8 FDA DNA O6 MeG 4 3 Mer MGMT O6 Paterson Trinity College PaTrinTM6 4 bromo 2 thenyl methoxy purin 2 amine PaTrin 2 O6 4 bromothenyl guanine