中药的抗肿瘤作用

1 1 1 S180 T NK I L 2 CTL 1 2 NK T 2 A K562 3 4 HepG2 He pG2 HepG2 G1 G0 S S G2 M S DNA DNA S PG PG c AMP c GMP PG 5 empirenews page 3 S G2 HL 60 2 DNA HL 60 24 6 5 0 g ml KB 72 41 9 KB G0 G1 7 PML RAR Bcl 2 8 HL 60 U937 9 4 DNA DNA 10 DNA G2 S 11 DMSO 2 3 g ml 80 g ml Pbr322 DNA 12 DNA DNA Bufalin Bufalin S 13 14 15 DNA 5 16 17 6 1 2 107 18 19 62 TNF T NF 20 empirenews page 1 2 3 IFN 21 TNF INF Lee 22 Bufalin 5 nmol L Bufalin FCM G2 M 23 10 g ml 0 1mg ml E p7 ras p21ras G GTP ras p53 p53 p53 p53 p53 25 p21ras p53 ras p53 bcl 2 bax bcl 2 bax 26 6T CEM bcl 2 bcl 2 bax 6T CEM 27 29 c myc c fos Rb 8 multidrug resistance MDR MDR P P gp 30 MTT KBv200 KB KBv200 K562 ADR K562 VCR MDR1mRNA P gp 31 BEL 7402 BEL 7402 DOX BEL 7402 P gp 32 empirenews page 9 33 10 50 34 35 36 10 1 1 996 27 2 1162 2002 102 223 A 2000 31 2 2074 2000 27 3 1885 2000 23 9 5546 1996 18 3 1697 2000 7 6 258 Chen GQ Zhu J Shi XG et al In vitro studies on cellular and molecular mechanisms of arsenic trioxide AS2O3 in the treatment of acute prom yeolcytic leukemia AS2O3 induces NB4 cell apoptosm with down regulation of bcl 2 expression and modulation of PMLRARd PML proteins 1996 88 3 10529 1999 19 3 3710 2000 3 15011 Liu LF Desai SD Li TK et al Mechnism of action of camptothecin Ann N Acad Sci 2000 92 2112 DNA DNA 2000 19 8 66113 Bafalin 2000 27 4 20 114 DNA K562 1999 18 6 661 empirenews page 15 HL 60 DNA 1999 20 6 54116 Kaye SB Progress in the treatment of ovarian Cancer Anti Cancer Druges 1999 9 2001 23 4 5218 Folkman J Clinical applications of research on angiogenesis N Eng J Med 1995 333 2000 11 4 17520 1992 31721 1 2 3 1994 1 3422 Lee DY Yasuda M Yamamoto T et al Bufalin inhibits endothelial cell proliferation and angiogenesis in vitro Life Sci 1997 60 2 127 23 2000 20 4 31324 2004 12 1 272 5 p21ras p53 2001 21 10 73 626 T 2000 41 3 17627 K562 2002 21 4 37028 H22 Ca C a Mg ATP c fos 2000 4 42229 HepsG2 2