北大生化考研

2009 1 2 C14 C14 3 GFP 4 DNA RNA RNA DNA DNA 6 ATP 7 8 9 0 5 mg KD 10 2006 1 2 A 3H B A 3H C 3H 3 4 5 G0 G1 S 6 COA NADH FADH2 A B C 16C GS 7 8 hybridization 9 DNA 2004 1 2 3 HbA 4 5 6 7 Ser Thr Tyr His Arg 8 1 6 3 9 1 6 10 DNA 11 12 Asp Glu Tyr Arg 13 mRNA tRNA rRNA 14 Vc SH 15 SH 18 19 O N 20 A B 21 FADH2 NADH 22 23 AA 24 25 26 27 Rubbissco A B 28 C4 A B 29 30 N NH3 A B C D 31 32 Glu Gln 33 34 RNA 35 64 36 37 tRNA 38 39 40 41 2 42 Cys 43 44 a B 45 Aufinsen 8mol 46 T 47 48 Tim Tom 1 1ug M 92 10 0 5umol 2 Ala 2Cys Asp Lys Ser Gly Phe DNFB DNP Ala Asp DNFB DNP gly 3 CoA 1 2 3 Km 1 4 cAMP PKA 5 Glu 1 3 6 7 8 I A 21aa B 30aa aa 1 2 II 1 3 4 5 6 2003 1 RNA DNA 2 3 RNA mRNA 4 18C 5 G G CoA G 1 G 6 7 8 9 5 FU 6 10 2001 1 replisome kaoyangj 2 3 antitermination factors 1 4 synonym codon 5 positive cooperativity 471 6 electron sink 7 Fe s protein 8 phosphoryl group transfer potenial kaoyantj 9 phospholation 10 ribonucleotide diphosphate reductasekaoyantj 8 1 Ala His Ser Glu Lys PH4 5 6554 8814 2 mRNA 3 Vmax 25 mol L min Km 1 25 10 5mol L Km S 5 0 10 6mol L V 4 B1 5 6 ATP H2O AMP P P 65976 455 7 2000 1 1 chemical shift 2 florescence anisotropy 7 spin labelling 4 dichroicratio 9 relaxation time 6 lipidpolymorphism 10 phase separation 8 active oxygen 11 secondary structure 10 sensitized fluorescence 16 adtivc transport 12 ion pump 17 ion channel 14 patch clamp 18 ceii adhesion 16 free radical 17 modeling 18 feedback 19 biocybernetics 20 information processing 1 2 3 4 5 6 7 8 9 10 1998 1 2 3 4 5 Asp Leu Lys sulphonoted polysturene PH5 6 A PH 7 0 B PH 6 0 H 7 0 01N PH 3 4 0 01N HCl PH 2 0 01N NaOH 15mL NaOH 8 I U 30 C 9 PH5 0 DEAE 2 2U mg 303U mg 10 10 5 10 turnovern umber 11 12 13 A S A A 14 Km 3X10 5M Vmax 8000 3X10 5M 15 2 2 Km 16 17 18 19 20 21 22 17 23 3 5 cAMP 24 25 26 27 U N 28 29 30 RNA DNA 31 DNA Maxam Gilbert 32 DNA 33 RNA III 34 DNA RNA 35 36 mRNA cDNA 37 Ile Ile Val 38 39 DNA C 5 C CG AG 40 41 DNA 42 5 43 mRNA G 29 C 44 45 1 2 3 4 5 B12 6 7 FeCl3 8 9 1 29 Glu Val Lys Asn Cys Phe Arg Trp A sp Leu Gly Ser Leu Glu Ala Thr Cys Arg His Met Asp Gln Cys Tyr Pro Gly Glu Glu Lys a b PH7 5 pKa c 2 DS 3 PH PH 4 PH 11 4 a hydrolysis f reduction of a double bond b Oxidative decarboxylation g isomerigation c phosphorolysis h dehydration d non oxidative decarboxylation i condensation e thiolysis j phosphorylation 5 PH2 H PH7 H G RT 620 mol ATP H ATP H ATP 7 3 ATP 10 3M ADP 10 5M 10 3M 6 T1 Trna pA pC pU U 1 2 2 1 tRNA 3 7 S Km 10 1 8 cDNA cDNA EcoR I EcoR I DNAsouthern DNA BamHI cDNA BamH I southern EcoR I BamH I 1995 doc 1 dna tenin 1970 rous rna r 1 t 2 3 11 2 12 13 14 1 8 atp 6 15 d 16 1 dna pi 2 17 18 prpr 1 5 1 5 1 2 3 4 5 1 a b c d e 2 a 3 b 3 c d e 3 a b c d e 4 g a b c d e 5 a h b h c h d ph e pi adp atp 6 gga mrna a 5 gga 3 5 3 b 5 gga 3 3 5 c 3 cca 5 5 3 d 3 cca 5 3 5 7 co2 a b c6 c c5 d c4 e c2 8 a b c d e 19 a dna b c d dna e dna 20 a b c d e 21 a b c d e 22 cap a b c d e 23 ph pk 1 6 2 8 3 e 10 5 a 5 b 6 c 7 d 8 e 9 14 a 7 b 11 c 7 11 d 7 e 11 15 a rh b c 3 d rh 3 e 3 16 a b c d e 17 d a c1 c25 b c1 c25 c c1 c25 d c25 c1 e c25 c1 18 a b c d e 1 19 a b c