串联质谱法测定兔血浆中葛根素的浓度.doc

,AglientC18(150mm2.1mm,3.5m),10mmolL-1(702010),0.2mLmin-1ESI103000ngmL-1(r=0.9986),10ngmL-1,99.91103.92,91.33100.40,(RSD)15,1.67min,;(puerarin),12-4,(LCMSMS),11. 1ABAPI3000;Waters20;TECHNE;WatersHLB(100mg)(,0.25g/5mL);(,:0752200108,0721200104);1. 2:AglientC18(150mm2.1mm,3.5m),:10mmolL-1(702010),0.2mLmin-1(ESI),(MRM),:m/z417.2267.2(),m/z611.2303.2(),:7500CCmin-1,:8Lmin-1,:8Lmin-1,:6Lmin-1,:5500V,:3501. 3,160gmL-1;,500ngmL-11. 41.0mL1.0mL,300L10mmolL-1300L20L,1.0mL,0.5mL,45N2,150L,5L22. 1(1),m/z417.2267.2m/z611.2303.2,2A2B2C,1.67min1.75min,1(1)(2)()Fig.1FullscanMS-MSspectraofpuerarin(1)andhesperidin(2)A. ;B.;C.1. m/z417.2267.2,tR=1.67min;2.m/z611.2303.2,tR=1.75min2()Fig.2ChromatogramsofpuerarinandhesperidininplasmabyLCMSMS2. 2,103010030010003000ngmL-1,1.4,:Y=0.00299C+000234,r=0.9986,1/X2103000ngmL-1,10ngmL-13. 3,1015002500ngmL-1,1.4,1d5,3d,3RSD7.56,4.43,9.66(n=5);RSD13.58,7.20,7.06(n=15);,3(103.9214.12),(99.91719),(100.297.08)(n=15)4. 41.4,1015002500ngmL-1150L(n=5),A1,A2,A3;5,1.4,A1,A2,A3,(n=5),(91.3311.06),(100.407.97),(96.366.28)5. 52500ngmL-15,51.4,(98.453.57)6. 635,-7552124h3,1.4,lt;15,;6h,lt;15,7. 76(),2.02.5kg,12h100mg,0.250.51234681012h2mL,(3500r/min)15min,33m/z417.2267.2,SPE,LCMSMS,WatersHLB,LC/MS/MS,10ngmL-1,2min,3100mg(n=6)()Fig.3Theconcentrationtimecurveofpuerarininrabbitplasmaafteriv100mg(n=6)1,.J.,1997,28(11):693.2,.HPLCJ.,2