U6_5S_miRNA 引物探针设计.doc

内参1. RNU6Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA NR_004394.1ORIGIN 1 gtgctcgcttcggcagcacatatactaaaattggaacgatacagagaagattagcatggc 61 ccctgcgcaaggatgacacgcaaattcgtgaagcgttccatattttRNU6-F CTCGCTTCGGCAGCACARNU6-R AACGCTTCACGAATTTGCGT2. 5SHomo sapiens RNA, 5S ribosomal 2 (RNA5S2), ribosomal RNA NR_023364.1ORIGIN 1 gtctacggccataccaccctgaacgcgcccgatctcgtctgatctcggaagctaagcagg 61 gtcgggcctggttagtacttggatgggagaccgcctgggaataccgggtgctgtaggctt 121 t5S-FTCTCGTCTGATCTCGGAAGCTA5S-RGCGGTCTCCCATCCAAGTAMiRNA 方案1RT primer adaptorCTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGXXXXXXForward primer adaptorACACTCCAGCTGGGXXXXXXXXXXXXXXUniversal Reverse primer TGGTGTCGTGGAGTCG方案2RT primer adaptorGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACXXXXXXForward primer adaptorGCGGCGGXXXXXXXXXXXXXXUniversal Reverse primer GTGCAGGGTCCGAGG1. hsa-miR-122序列：uggagugugacaaugguguuug长度：222. Hsa-miR-16序列：uagcagcacguaaauauuggcg长度：223. hsa-mir-93序列：caaagugcuguucgugcagguag长度：23方案1设计引物Primer Result:miRNAmiRNA sequence (5-3)PrimerPrimer Sequence (5-3)Length of primerTm of primerGC content of primerhsa-mir-122TGGAGTGTGACAATGGTGTTTGRT primerCTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCAAACACC440.522727272727Forward primerACACTCCAGCTGGGTGGAGTGTGACAATG29900.55hsa-mir-16TAGCAGCACGTAAATATTGGCGRT primerCTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCGCCAATA440.522727272727Forward primerACACTCCAGCTGGGTAGCAGCACGTAAAT29880.52hsa-mir-93CAAAGTGCTGTTCGTGCAGGTAGRT primerCTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTACCTGC440.545454545455Forward primerACACTCCAGCTGGGCAAAGTGCTGTTCGTG30940.57方案2 设计引物窗体顶端Primer Result:miRNAmiRNA sequence (5-3)PrimerPrimer Sequence (5-3)Length of primerTm of primerGC content of primerhsa-mir-122TGGAGTGTGACAATGGTGTTTGRT primerGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAAACA500.54Forward primerGCGGCGGTGGAGTGTGACAATG22720.64hsa-mir-16TAGCAGCACGTAAATATTGGCGRT primerGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGCCAA500.58Forward primerGCGGCGGTAGCAGCACGTAAAT22700.59hsa-mir-93CAAAGTGCTGTTCGTGCAGGTAGRT primerG