专外翻译0 yaodao.doc

专外翻译The adopted RP-HPLC method has been developed and successfully applied to other pharmacokinetic analysis of bioactive component from TCM (Zhang and Yang, 2010). Thus, with some modifications, the well-developed RP-HPLC coupled with liquidliquid phase extraction can be considered as a suitable method for this study. To obtain good quality chromatograms with a baseline separation of compounds as quickly as possible, we previously tested various HPLC columns and experimental conditions (solvent systems, detection wavelength and temperature) by trial and error and finally found the Ultimate column under the present conditions was most applicable due to its ultimate performance.Zhang et al. have developed a chromatographic method to quantitatively analyze PM-SG for quality control of Polygonum multiflorum, showing the intra-day and inter-day precision (RSD) of less than 1.01 and 2.88%, respectively, and the recovery of PM-SG ranging from 100.03 to102.62% with RSD less than 2% (Zhang et al., 2008).Comparatively, our present method also obtained a similar good performance and result of RP-HPLC analysis for PM-SG based on its similar intra- and inter-day precision (both 2.33%) and recovery (99.70101.50% with RSD 2.45%) to that of Zhang et al., indicating our chromatography was satisfactory for quantitative analysis of PM-SG and even suitable for the quality control. Moreover, to validate the reliability and reproducibility of this method, a complete validation study was performed for determination of PM-SG in plasma sample, as well as in all tissue samples (data not shown).In terms of the data obtained from each sample, it is concluded that this RP-HPLC coupled with liquidliquid phase extraction was reliable and reproducible with high specificity, sensitivity, precision, accuracy, recovery and stability. By using this chromatographic system, PM-SG in different samples were clearly detected and identified by its retention time (Fig. 2). It is worth noting that, when compared with the chromatogram of blank plasma (Fig. 2a), a new peak was observed in that of plasma sample after PM-SG administration at the retention time of 3.676 min (Fig. 2c). After mass spectrometric analysis in another study (data not shown), we found this peak corresponded to an -d-glucuronic acid conjugate of PMSG, which was presumably a metabolite of PM-SG. Further study is needed to determine how this metabolite is synthesized and what effect it has.反向色谱的使用已经被开发且成功的运用于其他的中药活性成分的药代动力学分析。(张和杨,2010)。因此,经过一定的修改,健全的反相结合液-液相萃取法可被视为一种对本研究合适的方法。为获得一个基线分离良好且尽可能快的的化合物的质量色谱图，我们以前试验了各种高效液相色谱柱和实验条件(溶剂体系,检测波长和温度通过尝试和错误,最终找到了在根据他的极性表现目前情况下是最适用的极性柱。张已经开发出一种对何首乌的色谱法定量测定分析用于PM-SG质量控制,结果显示日间和日内精密度（RSD）各自至少为1.01和2.88%,且PM-SG的恢复范围从100.03到102.62%,在RSD小于2%的条件下(张,2008)。相比之下,我们目前使用反向色谱的方法也得到了一个类似的好性能，与张的方法相比，在相同日内日间精密度下（均小于2.33%）以及恢复(99.70101.50% with RSD 2.45%) 。说明我们的色谱定量分析是满足PMSG的质量分析，而且适合的质量控制。此外,为验证该方法的可靠性和可重复性,需要进行PMSG血样完整的验证学，同时在所有的组织样。（数据未显示）从每个样本中获得的数据,得出的结论是反向色谱和液-液相萃取相结合的方法可靠性高、重现性好、灵敏度高、精度高、准确性好、恢复好和稳定性高。利用该色谱系统,不同的PM-SG样品十分容易的在