硕士生物仪器分析之气相色谱PPT课件

.,1,气相色谱GasChromatography(GC),.,2,简介特点基本理论仪器流程及主要部件应用发展趋势,.,3,Chromatography,Isatechniqueusedtoseparateandidentifythecomponentsofamixture.Worksbyallowingthemoleculespresentinthemixturetodistributethemselvesbetweenastationaryandamobilemedium.Moleculesthatspendmostoftheirtimeinthemobilephasearecarriedalongfaster.,Introduction,.,4,GasChromatography,Herethemobilephaseisanunreactivegas(egNitrogen)flowingthroughatube.Andthestationaryphaseisaninvolatileliquidheldonparticlesofasolidsupport.,Introduction,.,5,GasChromatography(GC)isnowamatureanalyticaltoolparticularlyusefulintheanalysisofvolatileandthermallystableanalytes.,Characteristic,.,6,气相色谱可用来分析能瞬间气化且不分解的、沸点0.1%),GCanalysisoffragrances,.,47,.,48,格拉斯(Grasse)世界香水之都,.,49,提问时间,.,50,FutureTrendsintheSeparationSciences,1.Miniaturization,ComparisonamongGCcolumns(inscale),ComparisonamongHPLCcolumns(inscale),packedcolumns,capillarycolumns,.,51,2.Neithertemperature,norpressure:electricalfield,GC,thecolumntemperature,HPLC,apressuregradientsolvents,electrodriven,electrophoretictechniques,(hybridtechniquesthatuseaspectsofchromatographyaswellasaspectsofelectrophoresis),FutureTrendsintheSeparationSciences,.,52,3.Hyphenatedsystems,LC-LC(suchas:aRP-18column+achiralcolumn)SFE-CESFE-SPE-HRGCLC-GCSPME-GC-MSSFE-SFC,FutureTrendsintheSeparationSciences,.,53,4.Theroleofthesolventinseparationsciences,FutureTrendsintheSeparationSciences,http:/www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000200005,.,54,5.Highspeedseparationtechniques,6.Automationoftheseparationtechniques,7.Unifiedchromatography,FutureTrendsintheSeparationSciences,.,55,Generalseparationapproachemployedinatypicalcomplexanalysis,.,56,1.Extractionofsolidsamples,ExtractionTechniques,SoxhletextractionMicrowaveextractionSonicationandsimilartechniquesPressurizedsolventtechnique,Supercriticalfluidextraction(SFE),Subcriticalwaterextraction(SWE),Acceleratedsolventextraction(ASE),.,57,2.Extractionofliquidsamples,Liquid-liquidextraction(LLE)Solid-phaseextraction(SPE)Solidphasemicroextraction(SPME)Stirbarsorptiveextraction(SBSE)Pressurizedsolventextraction(PSE),SFE,SWE,ASE,ExtractionTechniques,.,58,3.Extractionofgaseoussamples,Headspacetechnique,thestaticheadspace