多杀菌素高产菌株的选育和发酵条件的优化研究.doc

多杀菌素高产菌株的选育和发酵条件的优化研究 在线阅读整本下载分章下载分页下载【英文题名】Study on the Breeding of Spinosad Producing Strain and Optimization of Fermentation Conditions【作者中文名】梁艳;【导师】闻建平;【学位授予单位】天津大学;【学科专业名称】生物化工【学位年度】2007【论文级别】硕士【网络出版投稿人】天津大学【网络出版投稿时间】2008-11-19【关键词】多杀菌素; 刺糖多孢菌; 选育; 发酵优化; 动力学;【英文关键词】Spinosad; Saccharopolyspora spinosa; Mutagenesis; Fermentation Optimization; Kinetic model;【中文摘要】多杀菌素是一种新型生物杀虫剂,具有以下优良特性:杀虫谱广,作用方式独特,杀虫活性高,半衰期短,残留低,无抗药性,对人畜无害,环境污染少等。多杀菌素在农牧业生产中具有广阔的应用前景,该产品的开发具有良好的经济效益和社会效益。多杀菌素的两种活性组分spinosyn A和spinosyn D,是刺糖多孢菌经有氧发酵产生的。目前,筛选高产菌株和优化发酵条件是提高多杀菌素产量的主要方法,也是国内外学者研究的热点。本文通过筛选多杀菌素高产菌株和优化发酵条件,使多杀菌素的产量提高了438.5%。 本论文根据多杀菌素的生物合成途径和代谢调节机理,对刺糖多孢菌进行理性化选育。以刺糖多孢菌原始菌株为出发菌株,经过紫外诱变,以2 mg/L鼠李糖和1.5%丙酸钠为筛选剂,筛选出抗鼠李糖和丙酸钠的多杀菌素高产突变株U-4213,通过前体正丙醇的添加实验,证明突变株对前体的耐受性增加,产素能力提高,产量达到120 mg/L,较出发菌株(产量为32.5 mg/L)提高了269.2%,且遗传性状稳定。 以突变株U-4213为出发菌株,通过一系列的单因子实验、Plackeet-Burman筛选实验和中心组合实验对培养基组分和发.【英文摘要】Spinosad, a novel kind of biopesticide, has following excellent characteristics: effective on a wide variety of crops pest, unique mode of action, active to economically important pests, short half-life, low residue, non-drug resistant, large margins of safety to mammals, mankind and environmental profile. Spinosad will be widely used in agriculture and stockbreeding, and its exploitation has good economic and societal benefit. Spinosyn A and spinosyn D are the most active compositions of spinosad, which ar.【DOI】CNKI:CDMD:2.2008.186808刺糖多孢菌分批发酵动力学研究 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Study on the Formentation Kinetic Models of Saccharopolyspora spinosa【作者中文名】张竞立; 钱曙光; 廖美德; 侯学文; 徐汉虹;【作者英文名】ZHANG Jing-li1; QIAN Shu-guang2; LIAO Mei-de1; HOU Xue-wen3; XU Han-hong1 （1 Key Lab of Pesticide and Chemical Biology; Ministry of Education; Lab of Insect Toxicology; South China Agric.Univ.; Guangzhou 510642; China; 2 College of Science; 3 College of Life Science; China）;【作者单位】农药与化学生物学教育部重点实验室华南农业大学昆虫毒理研究室; 华南农业大学理学院; 华南农业大学生命科学学院; 农药与化学生物学教育部重点实验室华南农业大学昆虫毒理研究室 广东广州; 广东广州;【文献出处】华南农业大学学报(自然科学版), Journal of South China Agricultural University, 编辑部邮箱 2006年 03期期刊荣誉：中文核心期刊要目总览ASPT来源刊CJFD收录刊【关键词】刺糖多孢菌; 多杀菌素; 发酵动力学模型; 拟合;【英文关键词】Saccharopolysora spinosa; spinosad; the model of fermentation kinetics; fit;【摘要】利用摇瓶培养试验研究了刺糖多孢菌Saccharopolyspora spinosa的分批培养过程中生物量、培养液pH、葡萄糖消长、多杀菌素含量等因素的变化规律。利用MATLAB软件对试验数据进行非线性回归,拟合出了细胞生长动力学模型、基质消耗动力学模型和产物生成动力学模型,得到了细胞生长量、残糖量、多杀菌素产量在发酵过程中随时间变化的规律,相关系数分别为0.989 34、0.992 66和0.986 35.拟合曲线95%置信度区间分析的结果表明,该模型能够很好的反映出刺糖多孢菌分批发酵的过程.【英文摘要】An experiment was conducted,for Saccharopolyspora spinosa in the changes of biomass,residual carbohydrate content,spinosad content,the pH value in the cultural medium by the shaking flash.The kinetic models of batch fermentation by S.spinosa for biomass,substrate consumption and spinosad production were studied.The models were fitted and provided a useful aquation for biomass,substrate consumption and spinosad production.And the parameters of the models were obtained by using non-linear regretssion techniqu.【DOI】cnki:ISSN:1001-411X.0.2006-03-013多杀菌素发酵培养基的研究 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Study on the fermentation media for spinosad production【作者中文名】李能威; 张晓琳; 郭伟群; 熊犍; 宋炜; 宋渊;【作者英文名】LI Neng-wei1; 2; ZHANG Xiao-lin1; GUO Wei-qun1; XIONG Jian2; SONG Wei1; SONG Yuan3（1 Academy of State Administration of Grain; Beijing 100037; China; 2 Light Industry and Food College; South China University of Technology; Guangzhou; Guangdong 510640; 3 College of Biological Sciences; China Agricultural University; Beijing 100094; China）;【作者单位】国家粮食局科学研究院; 华南理工大学轻工与食品学院; 中国农业大学生物学院;【文献出处】西北农林科技大学学报(自然科学版), Journal of Northwest A & F University(Natural Science Edition), 编辑部邮箱 2010年 01期期刊荣誉：中文核心期刊要目总览ASPT来源刊中国期刊方阵CJFD收录刊【关键词】刺糖多孢菌; 多杀菌素; 发酵培养基;【英文关键词】Saccharopolyspora spinosa; spinosad; fermentation medium;【摘要】【目的】以刺糖多孢菌C4-10-8B为试验菌株,对多杀菌素发酵培养基进行优化,以提高刺糖多孢菌的多杀菌素发酵产量。【方法】采用单因素和多因素试验,分别考察了不同碳源、氮源、前体、无机盐和微量元素对多杀菌素生物合成的影响,最后通过正交试验综合考察发酵培养基中各组分对多杀菌素产量的影响。【结果】葡萄糖是较优碳源,最佳质量浓度为40.0 g/L,当葡萄糖与糊精(质量比52)复配时,多杀菌素产量较对照培养基提高18.35%;棉籽蛋白为最优氮源,25.0 g/L棉籽蛋白与15.0 g/L玉米蛋白胨或0.6 g/L硫酸铵复配时,多杀菌素产量分别较对照提高40.0%和45.3%;乙酸钠和谷氨酰胺质量浓度为1.0 g/L时,均能使多杀菌素产量较对照提高21.5%;K2HPO4对多杀菌素合成有明显的促进作用,在其质量浓度为2.5 g/L时,多杀菌素产量较对照提高24.5%;ZnCl2对多杀菌素合成也有促进作用,而MgSO4和CoCl2则显著抑制多杀菌素合成;通过2次正交试验获得最优发酵培养基,利用该培养基时多杀菌素产量可达395.54g/mL,比对照提高了62.5%。【结论】多杀菌素生物合成的最优发酵培养基为:葡萄糖50.【英文摘要】【Objective】 The fermentation media of Saccharopolyspora spinosa C4-10-8B were optimized for better spinosad production.【Method】 One-factor and multifactor experimental designs to optimize carbon sources,nitrogen sources,precursors,minerals and trace elements for the spinosad production were performed,and each selected element was discussed comprehensively with orthogonal designs for better spinosad-producing fermentation media.【Result】 The results showed that glucose was the optimum carbon source,and the op.【基金】国家“十一五”科技支撑计划项目子课题(No.2006BAD08B02)“高效生物储粮防护剂-多杀菌素的研制开发”【DOI】CNKI:SUN:XBNY.0.2010-01-034新型生物源杀虫剂多杀菌素 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【文献出处】江西棉花, Jiangxi Cotton, 编辑部邮箱 2010年 01期期刊荣誉：ASPT来源刊CJFD收录刊【摘要】多杀菌素是从放线菌代谢物中提纯出来的生物源杀虫剂,可使害虫迅速麻痹、瘫痪,最后死亡。具有胃毒和触杀两种作用,以胃毒为主。可用于防治蔬菜、棉花上的小菜蛾、甜菜夜蛾及蓟马等害虫。其毒性极低,杀虫速度快。喷药后当天即见效,可与化学农药相媲美。安全收获间隔期为1天,适合于无公害蔬菜生产。杀虫机理独特,与目前使用的各类杀虫剂没有交互抗性,是治理蔬菜抗性害虫的理想药剂。1防治对象及使用方法防治小菜蛾。在蔬菜莲座期,小菜蛾处于低龄幼虫期用药,用25 g/L多杀菌素水悬浮剂495750 ml/hm2,兑水300750 kg均匀喷雾。防治甜菜夜蛾。于低龄幼虫期用药,用25 g/L多杀菌素水悬浮剂7501 500 ml/hm2,兑水300750 kg喷雾。清晨、傍晚用药防虫效果最佳。防治蓟马。在蓟马发生期用25 g/L多杀菌素水悬浮剂495750 ml/hm2,兑水300750 kg均匀喷雾,重点喷洒幼嫩组织,如花、幼果、顶尖嫩梢。防治棉铃虫、烟青虫。于低龄幼虫期用药,用25 g/L多杀菌素水悬浮剂1 2001 500 ml/hm2,兑水300750 kg均匀喷雾。2注意事项该产品对水生节肢动物具.【DOI】CNKI:SUN:JXMH.0.2010-01-01696孔板高通量筛选多杀菌素高产菌株的研究 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Studies on High Throughput Screening for High Spinosad-producing Strains with 96-well Plates【作者中文名】罗莉斯; 李能威; 李丽; 郭伟群; 郁建平; 张晓琳;【作者英文名】LUO Li-si1; 2; LI Neng-wei2; LI Li2; GUO Wei-qun2; YU Jian-ping1; ZHANG Xiao-lin2（1.College of Life Sciences; Guizhou University; Guiyang 550025; 2.Academy of State Administration of Grain; Beijing 100037; China）;【作者单位】贵州大学生命科学学院; 国家粮食局科学研究院;【文献出处】中国农业科技导报, Journal of Agricultural Science and Technology, 编辑部邮箱 2010年 02期期刊荣誉：ASPT来源刊CJFD收录刊【关键词】刺糖多孢菌; 多杀菌素; 96孔板; 高通量筛选;【英文关键词】Saccharopolyspora spinosa; spinosad; 96-well plate; high throughput screening;【摘要】考察了刺糖多孢菌在96孔板固体培养基和液体培养基中的生长情况,建立了96孔板高通量筛选多杀菌素高产菌株的方法。结果表明:96孔板三明治盖能同时满足过滤杂菌和刺糖多孢菌生长耗氧所需,刺糖多孢菌在96孔板固体培养基中的生长情况与传统斜面培养相当,种子培养最适种龄为60h,每孔发酵培养基装液量为0.4mL。与传统摇瓶发酵筛选平台相比,该方法能显著提高多杀菌素高产菌株的筛选效率。【英文摘要】Based on the growth of Saccharopolyspora spinosa in both solid and liquid medium,a high throughput method was established to screen high spinosad-producing strains with 96-well plates.The results showed that applying the sandwich covers for 96-well plates could meet the requirement of bacteria filtration and oxygen supply for the growth of S.spinosa.S.spinosa grew well in both 96-well plate and slant culture.The optimum seed growing time was 60 h;the broth content of each well was 0.4 mL.Comparing with the .【基金】“十一五”国家科技支撑计划项目(2006BAD08B02-1)资助【DOI】CNKI:SUN:NKDB.0.2010-02-028多杀菌素对储粮害虫谷蠹磷化氢抗性和敏感品系的毒力比较 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Toxicity comparison of spinosad against phosphine-resistant strains and phosphine-susceptible strains of Rhyzopertha dominica (F.)【作者中文名】吴若旻; 王殿轩; 姜雪; 张晓琳; 曹阳;【作者英文名】WU Ruo-min1; WANG Dian-xuan1; JIANG Xue1; ZHANG Xiao-lin2; CAO Yang2（1.School of Food Science and Technology; Engineering Research Center of Grain Storage and Security of Ministry of Education; Henan University of Technology; Zhengzhou 450052; China; 2.Academy of State Administration of Grain; Beijing 100037; China）;【作者单位】河南工业大学粮油食品学院粮食储藏与安全教育部工程研究中心; 国家粮食局科学研究院;【文献出处】河南农业大学学报, Journal of Henan Agricultural University, 编辑部邮箱 2010年 02期期刊荣誉：中文核心期刊要目总览ASPT来源刊中国期刊方阵CJFD收录刊【关键词】谷蠹; 磷化氢抗性; 多杀菌素; 毒力;【英文关键词】Rhyzopertha dominica（F.）; phosphine resistance; spinosad; toxicity;【摘要】根据FAO推荐方法,采用滤纸药膜法比较测定多杀菌素对磷化氢抗性系数分别为343,128,56,1倍的谷蠹4个品系成虫(R343,R128,R56,R1)的毒力.结果表明,在24 h药膜接触处理后R343,R128,R56,R1的LD50值分别为25.269 7,8.163 2,7.590 4,9.564 5g.cm-2;经过48 h药膜接触处理后,R343,R128,R56,R1的LD50值分别为0.274 0,0.204 3,0.250 8,0.371 6g.cm-2.试验剂量下,分别经24 h和48 h药膜处理后存活的试虫,在适宜培养条件均在脱离接触后5 d时全部死亡.对磷化氢抗性存在明显差异的谷蠹品系对多杀菌素的敏感性差异不明显.说明对磷化氢有明显抗性的谷蠹对多杀菌素没有表现出交互抗性.【英文摘要】The toxicity of spinosad against four strains of Rhyzopertha dominica (F.)(R343,R128,R56 and R1) with different phosphine resistance factors of 343,128,56 and 1 times was bio-assayed by us filter paper and film.The results indicate that the LD50 values of R343,R128,R56 and R1 after keeping contact with the film for 24 h are respectively 25.269 7,8.163 2,7.590 4,9.564 5 g cm-2;and the LD50 values of R343,R128,R56 and R1 after keeping contact with the film for 48 h are respectively 0.274 0,0.204 3,0.250 8,0.【基金】国家科技支撑计划(2006BAD08B02)【DOI】CNKI:SUN:NNXB.0.2010-02-019琼脂块筛选法在多杀菌素菌种选育中的应用 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Application of agar column method in the screening of high-yield strain of spinosad by Saccharopolysporaspinosa【作者中文名】王德金; 田威; 傅瑶; 刘璇; 郝晓为; 何建勇;【作者英文名】WANG De-jin; TIAN Wei; FU Yao; LIU Xuan; HAO Xiao-wei; HE Jian-yong（School of Life Science and Biopharmaceutics; Shenyang Pharmaceutical University; Shenyang 110016; China）;【作者单位】沈阳药科大学生命科学与生物制药学院;【文献出处】沈阳药科大学学报, Journal of Shenyang Pharmaceutical University, 编辑部邮箱 2010年 04期期刊荣誉：中文核心期刊要目总览ASPT来源刊CJFD收录刊【关键词】多杀菌素; 琼脂块法; 菌种选育; 刺糖多胞菌;【英文关键词】spinosad; agar column method; strain improving; Saccharopolysporaspinosa;【摘要】目的创建一种新的、高效的多杀菌素菌种选育方法,提高菌种筛选的效率。方法采用琼脂块培养的方法进行单菌落的固体发酵,采用硅胶薄层层析法初步测定菌株的产量。结果琼脂块筛选法与摇瓶发酵筛选法具有相关性。琼脂块的最适培养时间为11 d,硅胶薄层层析法检测多杀菌素的最低质量浓度为100 mg.L-1。结论采用琼脂块筛选法进行多杀菌素高产菌种的选育,可显著提高筛选效率。【英文摘要】Objective To create a new and high efficiency strain screening method for improving the efficiency of screening bacteria.Methods Agar column method was used in the solid fermentation by a single colony;the thin layer chromatography(TLC)was used for the primary detection of spinosad.Results The correlation between agar column method and flask fermentation method was showed.The optimum culture period for agar column was 11 d;the minimum detectable concentration of spinosad by TLC method was 100 mgL-1.Conclus.【DOI】CNKI:SUN:SYYD.0.2010-04-017乙基多杀菌素 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】spinetoram【文献出处】农药科学与管理, Pesticide Science and Administration, 编辑部邮箱 2010年 07期期刊荣誉：ASPT来源刊CJFD收录刊【摘要】理化性质:乙基多杀菌素是从放线菌刺糖多孢菌(saccharopolyspora spinosa)发酵产生的,是多杀菌素(spinasad)的换代产品。其原药的有效成分是乙基多杀菌素-J和乙基多杀菌素-L的混合物(比值为3:1),他们的杀菌生物活性无显【DOI】CNKI:SUN:NYKG.0.2010-07-025甲维盐多杀菌素3%乳油高效液相色谱分析 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】HPLC Analysis Research of 3% Emamectin Benzoate Pleocidin EC【作者中文名】任晓静;【作者英文名】Ren Xiaojing（Hebei Yihaiangenuo Agrochemical Co.; Ltd; Shijiazhuang 052160; China）;【作者单位】河北益海安格诺农化有限公司;【文献出处】农药科学与管理, Pesticide Science and Administration, 编辑部邮箱 2010年 12期期刊荣誉：ASPT来源刊CJFD收录刊【关键词】甲维盐; 多杀菌素; 高效液相色谱; 分析;【英文关键词】emamectin; benzoate; pleocidin; HPLC; analysis;【摘要】本文采用高效液相色谱法,以甲醇+乙腈+水(0.5%乙酸钠)为流动相,使用Nova-Pak C18 4m为填料的不锈钢柱和紫外波长检测器,检测波长250nm,对甲维盐.多杀菌素3%乳油进行了定量分析。结果表明,方法的标准偏差分别为0.007、0.019,变异系数为0.575%、0.790%,平均回收率为100.10%、100.43%,线性相关系数为0.999、0.998。【英文摘要】The essay use HPLC to analysis 3% emamectin benzoate pleocidin EC,use methanol,acetonitrile and water(0.05% acetic sodium) as mobile phase,Nova-Pak C18 4m as stainless steel pillar stuffed,UV wavelength detector,and wavelength detect as 250nm.The result shows this method standard deviation as 0.007、 0.019,coefficient of variation as 0.575、 0.790,average recovery rate as 100.10、 100.43,linear correlation coefficient as 0.999、 0.998.【DOI】CNKI:SUN:NYKG.0.2010-12-014MNNG对多杀菌素产生菌的诱变效应 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Mutagenic Effects of MNNG Treatment on the Spinosad Producing Strains【作者中文名】宋炜; 熊犍; 郭伟群; 张晓琳; 宋渊;【作者英文名】SONG Wei（1; 2）; XIONG Jian1; GUO Wei-qun2; ZHANG Xiao-lin（2*）; SONG Yuan3 （1.College of Light and Food Industry; South China University of Technology; Guangzhou 510640; 2.Academy of State Administration of Grain; Beijing 100037; 3.College of Biological Sciences; China Agricultural University; Beijing 100193; China）;【作者单位】华南理工大学轻工与食品学院; 国家粮食局科学研究院; 中国农业大学生物学院;【文献出处】中国生物防治, Chinese Journal of Biological Control, 编辑部邮箱 2009年 02期期刊荣誉：中文核心期刊要目总览ASPT来源刊CJFD收录刊【关键词】刺糖多孢菌; 多杀菌素; N-甲基-N 硝基-N-亚硝基胍（MNNG）; 诱变;【英文关键词】Saccharopolyspora spinosa; spinosad; N-methyl-N -nitro-N-nitrosoguanidin; mutation;【摘要】多杀菌素(spinosad)是刺糖多孢菌Saccharopolyspora sptnosa发酵产生的次级代谢产物,有显著杀虫活性。采用N-甲基-N硝基-N-亚硝基胍(MNNG)作为诱变因子对刺糖多孢菌的孢子进行诱变处理,以高效液相色谱方法检测多杀菌素的发酵产量。研究结果表明:诱变剂量为2mg/ml,诱变40min时多杀菌素产量有较大幅度提高。通过诱变最终得到5株产量分别比出发菌株提高61.1%、80.3%、128.1%、69.9%和77.8%的突变株。【英文摘要】Spinosad is a secondary metabolite with noticeable insecticidal activity maded from fermenta-tion of Saccharopolyspora spinosa.The spores of Saccharopolyspora spinosa were mutated with N-methyl-N -nitro-N-nitrosoguanidin(MNNG),and the yields of spinosad were detected by HPLC.Results showed that positive mutants with larger proportion were obtained when the spores of the parental strain were treated 40 min using 2 mg/ml MNNG.As a result,five high-producing strains were selected,and their spinosad titers wer.【基金】国家十一五科技支撑计划课题(2006BAD08802)【DOI】CNKI:SUN:ZSWF.0.2009-02-017刺糖多胞菌合成多杀菌素的基因研究 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Study on Spinosad Synthetic Genes in Saccharopolyspora spinosa【作者中文名】叶丽娟; 王辂;【作者英文名】YE Li-juan; WANG Lu （Department of Pharmaceutical Chemistry; Sichuan Industrial Institute of Antibiotics; China National Pharmaceutical Group Corporation; Chengdu 610052; China）;【作者单位】中国医药集团四川抗菌素工业研究所;【文献出处】国外医药(抗生素分册), World Notes on Antibiotics, 编辑部邮箱 2009年 04期期刊荣誉：ASPT来源刊CJFD收录刊【关键词】多杀菌素; 刺糖多胞菌; 生物合成; 基因; 聚酮合成酶; 大环内酯;【英文关键词】spinosad; Saccharopolyspora spinosa; biosynthesis; gene; PKS; macrolide;【摘要】多杀菌素是由刺糖多孢菌产生的一种新型大环内酯类混合次级代谢产物,为新型、高效、安全的生物杀虫剂。该类化合物由一个12元大环内酯以及一个中性糖和一个胺糖构成。多杀菌素生物合成基因大部分聚集在刺糖多孢菌基因组上约80kb大小的区域中。该区域DNA已被完全测序,并通过破坏目的基因的方法,对这一区域DNA的特性和功能进行了深入研究。多杀菌素生物合成基因簇包括5个编码I型聚酮合成酶的基因和14个与大环内酯结构修饰有关的基因,另外还有4个编码鼠李糖的基因未包含在上述基因簇中。【英文摘要】As a serial of novel, efficient and safe bio-insecticides, spinosad are produced by Saccharopolyspora spinosa and are catalogued into macrolide secondary metabolites. These compounds are composed by one neutral sugar (rhamnose), an amino sugar (forosamine), and a 5,6,5-tricyclic ring system fused to a 12-membered macrocyclic lactone. In the bacteria producing these compounds, most of the macrolide biosynthetic genes clustered together in a 7080kb region of the genome. DNA in this region has been sequenced .【DOI】CNKI:SUN:GYKS.0.2009-04-004新型生物源杀虫剂多杀菌素 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【作者中文名】张树正; 张春生; 冉德才;【作者单位】肥城市安庄镇农技站; 肥城市安庄镇林果站;【文献出处】农业知识, , 编辑部邮箱 2009年 34期期刊荣誉：ASPT来源刊中国期刊方阵CJFD收录刊【摘要】多杀菌素是从放线菌代谢物中提纯出来的生物源杀虫剂,可使害虫迅速麻痹、瘫痪,最后死亡。具有胃毒和触杀两种作用,以胃毒为主。可用于防治蔬菜、棉花上的小菜蛾、【DOI】CNKI:SUN:NYZS.0.2009-34-044大孔吸附树脂提取多杀菌素的方法 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Isolation and Purification of Spinosyns by Macroreticular Resin Adsorbent Method【作者中文名】胡西洲; 贺玉平; 戴经元; 林开春;【作者英文名】HU Xi-zhou（1; 2） HE Yu-ping（1） DAI Jing-yuan（1） LIN Kai-chun（1）（1）Wuhan Natures Favour Bioengineering Co.; Ltd.; Wuhan 430070; China; （2）College of Plant Sicence and Technology; Huazhong Agriculture University; China）;【作者单位】武汉天惠生物工程有限公司; 武汉天惠生物工程有限公司 武汉; 华中农业大学植物科技学院;【文献出处】华中农业大学学报, Journal of Huazhoung Agricultural University, 编辑部邮箱 2006年 04期期刊荣誉：中文核心期刊要目总览ASPT来源刊中国期刊方阵CJFD收录刊【关键词】多杀菌素; 大孔吸附树脂; 分离纯化;【英文关键词】spinosys; marcoreticular resin; isolation and purification;【摘要】采用大孔树脂吸附法提取多杀菌素。通过树脂选型,筛选出XAD-4作为多杀菌素提取的吸附剂;吸附最适pH值为11.0,流速为1/6(1/min),加入2%氯化钠;采用丙酮梯度解吸,总收率达64.7%。【英文摘要】In this dissertation,process of isolating and purifying spinosad from fermentation broth by macroreticular adsorbent resin was determined.XAD-4 nonpolar resin was screened as the best adsorbent,and adsorbent conditions were optimized under the following conditions: the pH was maintained at 11,flow rate of adsorption was 1/6(1/min),2.0% NaCl was feasible,and acetone was used for eluting spinosad.As a result,the recovery rate was 64.7%.【基金】“十五”国家科技攻关计划项目(2001BA509B18)资助【DOI】cnki:ISSN:1000-2421.0.2006-04-013多杀菌素的作用机理及其抗药性的研究进展 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Mode of Action and Resistance of Spinosad【作者中文名】王彦华; 王鸣华;【作者英文名】Wang Yanhua; Wang Minghua; Li Wenhong; Ma Chongyong（Key Laboratory of Monitoring and Management of Plant Diseases and Insects; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095; China）;【作者单位】南京农业大学农业部病虫监测与治理重点开放实验室; 南京农业大学农业部病虫监测与治理重点开放实验室 南京;【文献出处】农药科学与管理, Pesticide Science and Administration, 编辑部邮箱 2006年 11期期刊荣誉：ASPT来源刊CJFD收录刊【关键词】多杀菌素; 烟碱型乙酰胆碱受体; -氨基丁酸受体; 抗药性;【英文关键词】spinosad; nicotinic acetylcholine receptor（nAChR）; g-aminobutyric acid receptor（GABAR）; resistance;【摘要】多杀菌素是一类新的杀虫剂,具有高效、低毒、选择性强、对环境安全的特点。其作用机制是通过激活烟碱型乙酰胆碱受体(nAChR),使正常昆虫神经细胞去极化,也可通过抑制-氨基丁酸受体(GABAR)使神经细胞超极化。本文综述了多杀菌素作用机理及其抗药性的研究进展。【英文摘要】Spinosad is a novel insecticide which shows high efficency,good selectivity and safety to environment. Spinosad depolarizes insect neurons by activating nicotinic acetylcholine receptor(nAChR),and also acting at -aminobutyric acid receptor(GABAR)by inhibiting g-aminobutyric acid receptor to lead neurons hyperactive. The mode of action and resistance of spinosad were reviewed in this paper.【DOI】cnki:ISSN:1002-5480.0.2006-11-004新型微生物杀虫剂Spinosad(多杀菌素)的毒理学研究进展 推荐 下载阅读CAJ格式全文 下载阅读PDF格式全文 【英文篇名】Advances in toxicological research o