LED光源在园艺与药用植物和人工虫草室内培养中的应用

LED光源在园艺与药用植物和人工虫草室内培养中的应用,华南师范大学生命科学学学院李海航,博士、教授、博导2014年6月21日，广州,广东省植物生长调节剂协会与广州市果树科学研究所2014年植物生长调节剂与现代农业研讨会,植物的种子萌发、幼苗生长、形态建成、开花结果等过程都需要光,不同的生物及不同生长发育期需要不同的光质与光强,降温,光在植物与食用菌等的室内培养中广泛应用,光照,研究内容非洲菊组织培养不定芽诱导、芽增殖和生根铁皮石斛和金钗石斛组培繁殖原球茎增殖、生长、分化虫草子实体形成,生长,功能成分子实体的诱导形成生长：高、鲜重、干重生物活性物质:虫草素,多糖,类胡萝卜素,I-Gerberahybrida组织培养,XidianLiu刘西典,JianzongPeng彭建宗,XiaojingWang王小菁,Resultsshootmultiplication,Explant:,Dedifferentiation,callus,Redifferentiation,Singleshoot,Clustershoots,Rooting,Plantlets,MultiplicationDivision,Adventitiousbuds,LEDtreatment,Shootmultiplicationprocedure,ComparedtoWFL,nosignificantdifferenceswereobservedinthetreatmentsofV1,V2,V5andV6.V3andV4decreasedtheFWoftheshoots.V6increaseddryweightsignificantly.,Freshweightanddryweight,Heightandnumberofshoots,AllLEDscouldincreasetheheightofshootsexceptV5.ButNo.ofshoots/explanthadnosignificantdifferent.,Chlorophyllcontent,AllLEDsinducedchlorophyllbiosynthesissignificantly,comparedwithWFL,W,V1,V2,V3,V4,V5,V6,WFL,35daysculturedinvitrounderLED,V1V4,V3V6,V2V5WFL,35daysculturedinvitrounderLED,Explant:,Dedifferentiation,callus,Redifferentiation,Rooting,Plantlets,ResultsRootinduction,ReceptacleMultiplicationDivision,Adventitiousbuds,ClustershootsRootinductionunderLED,Singleshoot,V3treatmentdecreasedFWandDW.,V2treatmentincreasedFWandDWsignificantly.,FreshweightandDryweight,HeightsofshootsunderV1,V2,V3andV4weresignificantlyhigherthancontrol.,V2hadthemostnumberofrootscomparedwithothers.,Shootheightandrootnumber,V3,V4andV5promotedtherootactivitysignificantly.V2increasedthemaximumrootlength.,Rootactivityandmaximumrootlength,V1,V2,V3,V4,V5,V6,WFL,Seedlings35dafterrootinginduction,Survivalrate,Height(cm),No.ofleaves,V1V2V3V4V5V6WFL,100%100%100%100%100%100%100%,9.460.22a9.770.22a8.190.34b9.540.30a8.020.27b8.250.48b7.090.23c,5.190.23b4.940.11b6.060.19a6.000.35a5.310.24b6.190.20a4.600.27b,ResultsTransplantation,15daysaftertransplantation,nodifferenceonseedlingssurvivalrates.AllLEDtreatedseedlingsweresignificanthigherandnumberofleavesincreased.,V4,V1V5,V2V6,V3WFL,Transplantedseedlingsgrownfor15days,Among6LEDs,V1,V2andV6weresuitableforshoot,multiplicationandV2wasgoodforrootinduction.,SurvivalratesofseedlingsfromallLEDtreatments,hadnodifferenceafterseedlingtransplantation.ButtheseedlingsgrownunderLEDswerehigherwithmoreleavesthanWFL.,Conclusions,JieYe叶婕,HongmeiBao包红梅,QingshengYe叶庆生,II-Dendrobiumofficinale组培繁殖,Tissuecultureprocess,shoots,Plantlets(1-2cm),seedling(7-8cm),germination1month,growthanddifferentiation2month,seedsgrowth1month,protocormsshootelongation3month,Plantlets,27,LEDtreatment,FWofplantlet(g),bcabccc,After90daysgrowth,FWofplantletunderV6wasthehighest,buttherewerenosignificantdifferencescomparedtocontrol.,30days,60days,90days,0.10.0,0.2,0.3,V1V3,V2V4,V6,V5FL,a,dd,a,aba,a,b,ababa,ab,accbcab,FWofplantlets,V6,DWofplantlet(g),DWofplantletsunderV6wassignificantlyhigherthanothersafter90daystreatment.,30days,60days,90days,0.00,0.01,0.02,0.03,abab,ab,b,a,aa,a,aa,a,V1,V2,V3,V4,V6,V5FL,ab,a,c,abcabaaab,DWofplantletsc,V6,Heightofplantlet(cm),Thereweresomedifferentinplantletheightafter30and60daysirradiationunderLED,thedifferentdisappearedafter90days.,30days,60days,90days,0.50.0,1.5,2.0,V1,V2,V4V6,V3V5FL,a,a,a,a,bab,a,a,cbc,aa,a,aa,bcbca,bcbc,Heightofplantletsa,90天后差异不显著,Stemdiameter,(cm),bcbccd,Similarresultswereobservedinstemdiameter,30days,60days,90days,0.0,0.2,0.3,V2V4V6,V1V3V5FL,d,cd,a,ab,ab,b,d,aba,aa,aab,ababab,a,b,Stemdiameter,V6,Tillering,Comparedwithothertreatments,numberoftilleringunderV6wassignificantlyhigherthanFL.,60days,90days,0,1,2,a,a,V1,V2,V3,V4,V6,V5FL,aa,a,a,b,bc,a,bcbc,d,c,a,Tillering,分蘖,V6,V6,Rootsofplantlet,After60dayscultivation,thereweremorerootsunderV6.,60days,90days,20,4,V1V3,V2V4,V5,V6,FL,aa,a,a,a,cd,a,abb,d,ab,c,a,Rootsnumberperplantletsa,Growthofplantsafter30,daysunderLED.,Growthofplantsafter60days,cultivationunderLED.,Growthofplantsafter90days,cultivationunderLED.,Among6LED,V6weresuitableforplantletgrowth,itpromotedtheDW,diameter,tilleringandrootinginDendrobiumofficinale.,Conclusions,III-Cordycepsmilitaris,ZhiliYi易智莉,HaihangLi李海航,本实验室培养的蛹虫草,原基诱导形成,材料与方法,材料与方法,子实体生长实验,材料与方法,甲醇浸提,LED光照处理,结果与讨论,1.黑暗、红光、远红光下不能形成子实体原基,2.蓝光和含蓝光的LED组合都能诱导原基形成。表明蓝光在子实体原基诱导形成中起决定性作用,3.LED白光（R/B=2/1）和LED处理（R/B=2/1）对原基的诱导形成较好,1.光质对子实体原基形成的影响,处理鲜重最高,其他LED处理组与荧光对照组无显著差异,远红光下鲜重最低,结果与讨论,2.光质对子实体生长（鲜重）的影响,（R/B/Fr=4/2/4）干重较高,远红光下干重最低,其次是红光,-处理都含蓝光，干重无显著差异，但都高于远红光和红光,结果与讨论,3.光质对子实体生长（干重）的影响,结果与讨论,各处理的子实体平均高度无统计学差异,处理的高度略高于其它,4.光质对子实体生长（平均高度）的影响,I.FR(100%)II.R(100%),VII.R:B:FR1:6:3VIII.B(100%),III.R:B:FR4:2:4IV.R:B:FR3:2:5,V.R:B:FR3:3:4VI.R:B:FR1:4:5,IX.LEDwhiteX.Fluorescent,LED白光虫草素含量最高，比对照高62%；其次是LED红光，比对照高60%,其他LED组合比荧光对照都有不同程度的提高。,红光有利于提高虫草素的含量，蓝光对虫草素积累的作用不显著。,结果与讨论,5.光质对虫草素含量的影响,各光照处理下虫草多糖的含量无统计学差异。,结果与讨论,6.光质对虫草多糖含量的影响,LED蓝光下类胡萝卜素含量最高，比对照组提高了51.6%。,LED远红光下类胡萝卜素含量最低，比荧光对照低54.3%。其次是红光，比对照低46.4%。,蓝光和含蓝光的LED处理，类胡萝卜素都比对照高，且蓝光比例越高，类胡萝卜素含量越高。,结果与讨论,7.光质对类胡萝卜素含量的影响,光诱导色素合成,光诱导子实体原基形成（形态建成）,色素的形成发生在光照后4-4.5h内，至20h色素含量持续增加,结果与讨论,光诱导虫草色素形成的动态研究,光照1天内色素合成启动,色素合成的启动发生在光照后4-4.5小时内,结果与讨论,光诱导虫草色素形成的动态研究,蛹虫草色素HPLC-ESI-MS检测的总离子流图,色素成分与光诱导关键步骤待确定,结论,1.黑暗、红光、远红光下不能形成子实体原基；蓝光在子实体原基的诱导形成中起着决定性作用，蓝光和含蓝光的LED组合光都能诱导原基形成。,2.LED处理（R/B/Fr4/2/4）有益于子实体生长，单纯远红光和红光不利于子实体生长。其他