DNA固定在纳米颗粒表面的方法

磷酸化的DNABiotin-DNA炔基修饰的DNA巯基修饰的DNA氨基修饰的DNA,金银纳米颗粒碳纳米管、石墨烯二氧化硅纳米颗粒量子点上转换纳米颗粒,磷酸化的DNA与TiO2-堆积，如单链DNA与石墨烯，碳纳米管静电吸附,1、Biotin-DNA,2、炔基修饰的DNA及其他生物分子,3、NH2-DNA,氨基羧基,氨基羧基氨基氨基,1）EDC和NHS,Forbestresultsintwo-stepreactions,performthefirstreactioninMESbuffer(orothernon-amine,non-carboxylatebuffer)atpH5-6,thenraisethepHto7.2-7.5withphosphatebuffer(orothernon-aminebuffer)immediatelybeforereactiontotheamine-containingmolecule,氨基羧基,DCC（N,N-Dicyclohexylcarbodiimide）,氨基-氨基,1）BS(PEG)nPEGCrosslinkers-AmineReactive,2）TSAT(Tris-succinimidylaminotriacetate),Water,Water,NHSesters:pH7-9,3）DSS(disuccinimidylsuberate),4）BSOCOES(bis2-(succinimidyloxycarbonyloxy)ethylsulfone),5）DSG(disuccinimidylglutarate),6）DTSSP(3,3-Dithiobissulfosuccinimidylpropionate),Water,Water,Water,DMSOorDMF,8）BS(Bissulfosuccinimidylsuberate),9）EGSethyleneglycolbis(succinimidylsuccinate),10）Sulfo-EGSethyleneglycolbis(sulfosuccinimidylsuccinate),7）DST(disuccinimidyltartrate),Water,Water,DMSOorDMF,DMSOorDMF,4、巯基化的DNA,1Au-SAg-S2巯基氨基3巯基羧基4巯基羟基5巯基巯基,巯基-氨基,1）AMAS(N-maleimidoacetoxysuccinimideester),NHSesters:pH7-9；Maleimides:pH6.5-7.5；Usually7.2-7.5。Twostepsreaction,DMSOorDMF,2）BMPS(N-maleimidopropyloxysuccinimideester),DMSOorDMF,3）EMCS(N-maleimidocaproyloxysuccinimideester),DMSOorDMF,4）Sulfo-EMCS(N-maleimidocaproyloxysulfosuccinimideester),Water,6）Sulfo-GMBS(N-maleimidobutyryloxysulfosuccinimideester),5）GMBS(N-maleimidobutyryloxysuccinimideester),DMSOorDMF,Water,7）BMPA(N-maleimidopropionicacid),EDC，0.1molMESpH4.7Maleimidesreactwithsulfhydryls(SH)atpH6.5-7.5,8）LC-SMCC(Succinimidyl-4-N-maleimidomethylcyclohexane-1-carboxy-6-amidocaproate),DMSOorDMF,10）Sulfo-SMPB(sulfosuccinimidyl4-p-maleimidophenylbutyrate),9）SMPB(succinimidyl4-p-maleimidophenylbutyrate),DMSOorDMF,Water,11）Sulfo-LC-SPDP,LC-SPDP,SPDP,巯基-羧基,巯基-羟基,异氰酸脂在水溶液中很容易水解，所以最好用有机溶剂如DMSO、DMF等。如在水相中，则要使用pH8.5的碱性缓冲溶液，避免使用含羟基的缓冲液如tris缓冲液，且要注意含羟基的分子不能有氨基和巯基，因为pH7.5顺丁烯二酰亚胺失去对巯基的特异性，会同时与氨基和巯基发生反应。,