流式细胞仪试剂介绍PPT演示课件

流式细胞仪试剂介绍,五光十色的荧光素,单分子荧光素耦合的复合荧光素分子（TandemDye)新型的荧光素样荧光物质Qdots,常用激光和荧光素搭配,BlueFITCPEPerCPPerCP-Cy5.5PE-TexasRed(ECD)PE-Cy5PE-Cy7PIEGFP,RedAPCAPC-Cy7UVDAPIHoechst,2010,2005,2000,1995,1990,InnovationFirstCommercialTandemConjugates,1985,PE-Cy5.5,PE-TexasRed,PE-Cy7,PE-Cy5,APCAlexafluor700APCAlexafluor750,MulticolorFlowImpossibleWithoutTandems,BeckmanCoulter-PatentedConjugationProcess,PatentedProcessUniqueToBeckmanCoulter,NativeState,Unfold,CoupleAcceptorDye,Refold,Performance,Standardization,TandemVideo,UnpurifiedPC7DiscardedMaterialPurifiedPC7,PerformanceMatters:PatentedManufacturingProcess,FinishedTandemDyeThreeLotComparisonofPC7,Purification,ReproducibleDataOverTime,Performance,Standardization,PerformanceMatters:VendorComparison,ClearResolutionofCellularSub-sets,BeckmanCoulter,Performance,VendorX,VendorY,PerformanceMatters:ImpactOfPhoto-degradation,PatentedPackagingProtectsAgainstPhoto-degradation,Performance,荧光染料比较(平均荧光强度)Q-Prep/ImmunoPrep试剂系统,PerCPAPCFITCECD2Xlonger(90minutesvs.40minutes)Antibodiesneedseparateincubation:moresteps,iTAgMHCTetramerHigherS/Nratio,excellentpopulationseparationRapidprocedure,fastertimetoresultFewersteps,ManualIntracellularCytokines:Variability,WholeBlood18hrSEB+CD2830tubesatonetime:19replicatesCD4/IFNg/CD69,GatedonCD4+Lymphs:,DatekindlysuppliedbyDrWadeBolton,CustomBioPharmaServices,Miami,FL,USA,AutomatedIntracellularCytokines:Precision,WholeBlood18hrSEB+CD2830replicatesatonceCD4/IFNg/CD69,GatedonCD4+Lymphs:,DatekindlysuppliedbyDrWadeBolton,CustomBioPharmaServices,Miami,FL,USA,44,TheBeckmanCoulterAdvantage,MulticolorFlowMadeEasy,3,000products,USPatent6686004,USPatent6955872,Proprietarytechnol