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LessonOne,InsidetheLivingCell:StructureandFunctionofInternalCellParts,1.KnowingtheComponentofthecell.2.Describetherelationshipbetweencellshapeandcellfunction.3.Distinguishbetweenprokaryotesandeukaryotesstructure.4.Introducethestructuresandfunctionsofdifferenttypesofcellorganelles.,Glossary:Cyto-细胞Cytology:细胞学Cytoplasm:细胞质Cytokinesis:胞质分裂Cytoskeleton:细胞骨架Cytobiology:细胞生物学Cytocentrum:中心体,chloroplast:叶绿体chlorophyll:叶绿素carotenoid:类胡萝卜素:carotene:胡萝卜素lutein:叶黄素Accessorypigment:辅助色素,nucleus:细胞核(pl:nuclei)nucleoli:核仁nucleoid:拟核,类核nuclearenvelope:核膜nuclearpore:核孔,Pinocytosis:胞饮作用Phagocytosis:吞噬作用ribosome核糖体polysome多核糖体endoplasmicreticulum内质网Golgicomplex高尔基复合体lysosome溶酶体mitochondrion线粒体plastid质体,basalbody:基体cilia:纤毛flagella:鞭毛stroma基质,子座microfilament微丝,纤丝actin肌动蛋白myosin肌球蛋白microtubule微管,tubulin微管蛋白dynein动力蛋白chemotaxis趋化性cilia纤毛flagella鞭毛(单数flagellum)basalbody基体centriole中心粒,Cytoplasm细胞质Thelivingcontentsofacellboundedexternallybytheplasmalemma,includinganaqueousgroundsubstance(hyaloplasm,透明质,cellsap,orcellmatrix)containingorganellesandvariousinclusionsbutexcludingthenucleusandvisiblevacuoles.,Cytoplasm:TheDynamic,MobileFactoryMostofthepropertiesweassociatewithlifearepropertiesofthecytoplasm.Muchofthemassofacellconsistsofthissemifluidsubstance,whichisboundedontheoutsidebytheplasmamembrane.细胞质:动力工厂生命的大部分特征表现在细胞质的特征上。细胞质大部分由半流体物质组成,并由原生质膜包被。,Organellesaresuspendedwithinit,supportedbythefilamentousnetworkofthecytoskeleton.Dissolvedinthecytoplasmicfluidarenutrients,irons,solubleproteins,andothermaterialsneededforcellfunctioning.细胞器悬浮在其中,并由丝状的细胞骨架支撑。细胞质中溶解了大量的营养物质,离子,可溶蛋白以及维持细胞生理需求的其它物质。,nucleus细胞核,核(复数nuclei)Theorganelleoftheeukaryotecellthatcontainsthechromosomesandhenceultimatelycontrolscellularactivityandinheritancethroughtheactivityofthegeneticmaterial,DNA,TheNucleus:InformationCentral(细胞核:信息中心)Theeukaryoticcellnucleusisthelargestorganelleandhousesthegeneticmaterial(DNA)onchromosomes.(Inprokaryotesthehereditarymaterialisfoundinthenucleoid.)Thenucleusalsocontainsoneortwoorganelles-thenucleoli-thatplayaroleincelldivision.真核细胞的细胞核是最大的细胞器,细胞核对染色体组有保护作用(原核细胞的遗传物质存在于拟核中)。细胞核含有一或二个核仁,核仁促进细胞分裂。,Apore-performatedsaccalledthenuclearenvelopesepatatesthenucleusanditscontentsfromthecytoplasm.Smallmoleculescanpassthroughthenuclearenvelope,butlargemoleculessuchasmRNAandribosomesmustenterandexitviathepores.核膜贯穿许多小孔,小分子可以自由通过核膜,而象mRNA和核糖体等大分子必须通过核孔运输。,3.Organelles:SpecializedWorkUnits(细胞器:特殊的功能单位)Alleukaryoticcellscontainmostofthevariouskindsoforganelles,andeachorganelleperformsaspecializedfunctioninthecell.Organellesdescribedinthissectionincluderibosomes,theendoplasmicreticulum,theGolgicomplex,vacuoles,lysosomes,mitochondria,andtheplastidsofplantcells.所有的真核细胞都含有多种细胞器,每个细胞器都有其特定功能。本节主要介绍核糖体,内质网,高尔基体系,液泡,溶酶体,线粒体和植物细胞中的质体。,Ribosome核糖体Smallstructurescomposedoftwoproteinandribonucleicacidsubunitsinvolvedintheassemblyofproteinsfromaminoacids,Thenumberofribosomeswithinacellmayrangefromafewhundredtomanythousands.Thisquantityreflectsthefactthat,ribosomesarethesitesatwhichaminoacidsareassembledintoproteinsforexportorforuseincellprocesses.核糖体的数量变化从几百到几千,核糖体是氨基酸组装成蛋白质的重要场所。,Acompleteribosomeiscomposedofonelargerandonesmallersubunit.DuringproteinsynthesisthetwosubunitsmovealongastrandofmRNA,“reading”thegeneticsequencecodedinitandtranslatingthatsequenceintoprotein.完整的核糖体由大亚基和小亚基组成。核糖体沿着mRNA移动并阅读遗传密码,翻译成蛋白质。,SeveralribosomesmaybecomeattachedtoasinglemRNAstrand;suchacombinationiscalledapolysome.一条mRNA上可能有多个核糖体,称多聚核糖体。,Polysome多核糖体Ofproteinsynthesis,severalribosomesalltranslatingthesamemessengerRNAmolecule,oneaftertheother.,Mostcellularproteinsaremanufacturedonribosomesinthecytoplasm.Exportableproteinsandmembraneproteinsareusuallymadeinassociationwiththeendoplasmicreticulum.大多数细胞蛋白是由细胞质中核糖体生产。输出蛋白和膜蛋白通常与内质网有关。,Theendoplasmicreticulum,alacyarrayofmembranoussacs,tubules,andvesicles,maybeeitherrough(RER)orsmooth(SER).Bothtypesplayrolesinthesynthesisandtransportofproteins.TheRER,whichisstuddedwithpolysomes,alsoseemstobethesourceofthenuclearenvelopeafteracelldivides.内质网,带有花边的生物囊,有管状,泡状之分,以及光滑和粗糙面区别。两种都与蛋白质的合成和运输有关。粗糙内质网上分布许多核糖体,也可能提供细胞分裂后所需的细胞膜。,Endoplasmicreticulum内质网Foldedmembranesandtubesthroughouttheeukaryoticcellthatprovidealargesurfaceuponwhichchemicalactivitiestakeplace.,SERlackspolysomes;itisactiveinthesynthesisoffatsandsteroidsandintheoxidationoftoxicsubstancesinthecell.Bothtypesofendoplasmicreticulumserveascompartmentswithinthecellwherespecificproductscanbeisolatedandsubsequentlyshuntedtoparticularareasinoroutsidethecell.光滑内质网上无核糖体,主要作用是脂肪和类固醇的合成以及细胞内有毒物质的氧化。两种内质网合成的产物在其中进行分流或运输到细胞外。,Golgicomplex高尔基复合体Astackofflattened,smooth,memebranoussacs;thesiteofsynthesisandpackagingofcertainmoleculesineukaryoticcells.,Transportvesiclesmaycarryexportablemoleculesfromtheendoplasmicreticulumtoanothermembranousorganelle,thegolgicomplex,withintheGoligcomplexmoleculesaremodifiedandpackagedforexportoutofthecellorfordeliveryelsewhereinthecytoplasm.运输小泡能够将可运输的分子从内质网运输到高尔基复合体上。在高尔基复合体中修饰,包装后输出细胞或传递到细胞质中的其他场所。,Vacuolesincellsappeartobehollowsacsbutareactuallyfilledwithfluidandsolublemolecules.Themostprominentvacuolesappearinplantcellsandserveaswaterreservoirsandstoragesitesforsugarsandothermolecules.Vacuolesinanimalcellscarryoutphagocytosis(theintakeofparticulatematter)andpinocytosis(vacuolardrinking).细胞中的液泡好象是中空的,但实际上充满了液体和可溶分子。最典型的液泡存在于植物细胞中,储备水,糖以及其它分子。动物中的液泡起吞噬和胞饮作用。,Vacuole液泡Storagecontainerwithinthecytoplasmofacellhavingasurroundingmembrane.,Phagocytosis吞噬作用Theprocessbywhichthecellwrapsaroundaparticleandengulfsit.Pinocytosis胞饮作用Theprocessbywhichacellengulfssomemoleculesdissolvedinwater.,Asubsetofvacuolesaretheorganellesknownaslysosomes,whichcontaindigestiveenzymes(packagedinlysosomesintheGolgicomplex)thatcanbreakdownmostbiologicalmacromolecules.Theyacttodigestfoodparticlesandtodegradedamagedcellparts.溶酶体是液泡亚单位,含有消化酶,降解大部分生物大分子。消化食物微粒和降解损伤的细胞残片。,Mitochondrion线粒体Amembranousorganelleresemblingasmallbagwithalargerbaginsidethatisfoldedbackonitself;serveasthesiteofaerobiccellularrespiration.,Mitochondriaarethesitesofenergy-yieldingchemicalreactionsinallcells.Inaddition,plantcellscontainplastidsthatutilizelightenergytomanufacturecarbohydratesintheprocessofphotosynthesis.Itisonthelargesurfaceareaprovidedbytheinnercristae(嵴)ofmitochondriathatATP-generatingenzymesarelocated.线粒体是细胞中化学产能的场所。另外,植物细胞中的质体在光合作用中利用光能产生碳水化合物,线粒体内嵴上提供了很大的表面积并分布着产ATP酶。,Mitochondriaareself-replicating,andprobablytheyaretheevolutionarydescendantsofwhatwereoncefree-livingprokaryotes.线粒体自我复制,并且可能是自由生活的原核生物在进化中形成的后代。,Therearetwotypesofplastids:leucoplasts,whichlackpigmentsandserveasstoragesitesforstarch,proteins,andoils;andchromoplasts,whichcontainpigments.质体有两种类型:白色体,缺乏色素,是淀粉,蛋白质和油的储备场所;色质体,含有色素。,Themostimportantchromoplastsarechloroplasts-organellesthatcontainthechlorophyllusedinphotosynthesis.Theinternalstructureofchloroplastsincludesstacksofmembranescalledgrana,whichareembeddedinamatrixcalledthestroma.叶绿体是最重要的色质体,含有与光合作用有关的叶绿素。叶绿体的内部结构是由多层膜形成的叶绿体基粒,其中包埋在基质中的基粒称子座。,Chloroplast叶绿体Aplastidinwhichphotosynthesisiscarriedout.Chloroplastsoccurinallphotosyntheticorganismsexceptphotosyntheticbacteriaandblue-greenalgae.,Stroma子座,基质Regionwithinachloroplastthathasnochlorophyll.Plastid质体Anorganellepresentinallplantsexceptbacteria,blue-greenalgae,andfungi;itisenclosedbytwomembranes(theenvelope)andhasvariousfunctions.,TheCytoskeleton(细胞骨架)Alleukaryoticcellshaveacytoskeleton,whichisaconvoluted(回旋)latticework(格子状)offilamentsandtubulesthatappearstofillallavailablespaceinthecellandprovidessupportforvariousotherorganelles.所有的细胞都有细胞骨架,网络结构的纤丝充满了它所能触及的全部空间并且对细胞器提供支持作用。,Cytoskeleton细胞骨架Ofeukaryoticcells,aninternal“skeleton”.Itsmicrotubulesandothercomponentsstructurallysupportthecell,organizeandmoveitsinternalcomponents.Thecytoskeletonalsohelpsfree-livingcellsmovethroughtheirenvironment.,Alargeportionofthecytoskeletonconsistsofthreadlikemicrofilamentscomposedmainlyofthecontractileproteinactin.Theyareinvolvedinmanytypesofintracellularmovementsinplantandanimalcells.细胞骨架大部分由微丝组成,微丝主要由可收缩的肌动蛋白组成。动植物细胞的许多种类型细胞内运动与肌动蛋白有关。,Microtubule微管Small,hollowtubesofproteinthatfunctionthroughoutthecytoplasmtoprovidestrucutualsupportandenablemovement.Microfilament微丝,纤丝Long,fiberlikestructuresmadeofproteinandfoundincells,oftenincloseassociationwiththemicrotubule;providestructuralsupportandenablemovement.,Asecondprotein,myosin,isinvolveinthecontractionofmusclecells.Anothermainstructuralcomponentofthecytoskeletonconsistsofmicrotubules,whicharecomposedoftheglobularproteintubulinandtogetheractasscaffoldingthatprovidesastablecellshape.第二类蛋白是肌球蛋白,它与肌肉细胞的收缩有关。细胞骨架的另一个主要结构成分是微管,由球状的微管蛋白组成,象脚手架一般维持细胞的稳定形态。,ACytoskeletalintermediatefilamentsappeartoimparttensilestrengthtothecellcytoplasm.Mechanoenzymessuchasmyosin,dynein,andkinesininteractwiththecytoskeletalfilamentsandtubulestogenerateforcesthatcausemovements.细胞骨架的中间丝提供了细胞质伸缩动力。机械酶,例如,肌球蛋白,动力蛋白,驱动蛋白与微丝,微管相互作用产生动力而引起细胞运动。,CellularMovements(细胞运动)Althoughthecytoskeletonprovidessomestabilitytocells,itsmicrotubulesandfilamentsandtheirassociatedproteinsenablecellstomovebycreepingorgliding.尽管细胞骨架提供了细胞的某些稳定性,微丝,微管及相关蛋白能使细胞爬行或滑动。,Suchmovementsrequireasolidsubstratetowhichthecellcanadhereandcanbeguidedbythegeometryofthesurface.Somecellsalsoexhibitchemotaxis,theabilitytomovetowardorawayfromthesourceofadiffusingchemical.这种运动需要固体基质依托并通过表面几何形状的改变而运动。某些细胞具备趋化性,即趋向或逃离扩散开的化学源。,Chemotaxis趋化性Alocomotorymovementofanorganismorcellinresponseto,anddirectedby,anexternaldirectionalstimulus.,Certaineukaryoticcellscanswimfreelyinliquidenvironments,propelledbywhiplikeciliaorflagella.Bothciliaandflagellahavethesameinternalstructure:某些真核细胞能在液体中自由运动,由纤毛或鞭毛推动。纤毛和鞭毛具有同样的内部结构:,Cilia纤毛Numerousshort,hairlikestructuresprojecting(突出)fromthecellsurfacethatenablelocomotion.Flagella鞭毛Lo

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