世界针灸学大会报告：方便揿针原始效应物质研究

针刺原始效应物质的研究StudyonAcupunctureOriginalMaterial世界针灸学会联合会成立20周年庆典暨世界针灸学术大会讲演稿,张大同杨肖娥1王樟连2郑振洨王海明李廷强1冯英1沈瑾王芸2（杭州市第三医院，杭州310002；1浙江大学，杭州310027；2浙江中医药大学，杭州310053）(ZHANGdatong,YANGXiaoe1,WANGZhanglian2,ZHENGZhenxiao,WANGHaiming,LITingqiang1,FENGYing1,SHENJin,WangYun2（ThirdHospitalofHangzhou,310002；1ZhejiangUniversity,310027；2ZhejiangChineseMedicalUniversity310053,Hangzhou,ZhejiangProvince，PR.China）,目的将下述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，共同组成一个完整的证据锁链，证明针行方便揿针针刺原始效应物质、针刺电化学效应机制。,ObjectiveWewillpracticethefollowingFourexperimentsmadebytheevidence,electrochemicalandcorrosionoriginalmicro-batteryeffectscientificlaws,thecommoncompositionofacompletechainofevidence，andwillprovethattheeffectofacupuncturerawmaterials,electrochemicaleffectsofacupuncturemechanism.,实验1针行方便揿针针刺电化学电流研究实验1-1人体针刺电化学电流研究,Experiment1-1Electrochemicalcurrentstudybyacupuncture,方法将方便揿针分别垂直刺入实验对象的肩髃、肩髎穴位中，用数字显示电流仪测量记录产生的电流变化,MethodsAcupunctureneedleswerestungverticallytoalienateexperimentsofthepointsofJianyu,Jianliao,withfigurescurrentmeasurementrecordsshowedthechangesofthecurrent.,结果方便揿针刺入人体穴位，所测的9名对象电流变化在-4467A区间，电流差值为2773A。,ResultStainlesssteelacupuncturewereneedledintothehumanbody,measuringthechangesonthecurrenttargetis-4467uAinterval,thecurrentmarginwas2773Aonthecaveof9cases.,表1-1、针刺肩髃、肩髎穴的电流测定记录表（男=m、女=w）Table1、StatisticsofelectriccurrentmeasuredinthepointofJianYu、Jianliaoinserted（Man=m、Woman=w）,N性别年龄（岁）电流变化（A）电流差值（A）峰值出现（次）Sexage（years）electriccurrentelectriccurrentdifferencepeakvalue1w69-20133342m52-9435253w65-44186254m78-37165365w75-4232746w77-6677347m72-24487258w54-12395159m23-2547726,实验1-2动物针刺电化学电流研究,方法将12只ICR实验小鼠按其存活及处死（强力颈椎脱位法）后的整个时间过程分为：A组（健康活体），B组（死亡1h），及C组（死亡28h）；先将方便揿针分别刺入每只经麻醉的ICR实验小鼠左侧的曲池与后三里穴，用数字显示电流仪测量观察2min并记录产生的电流变化，后将该小鼠处死；待该小鼠处死1h、28h后再分别对其右侧的曲池与后三里穴，并用数字显示电流仪测量观察2min并记录产生的电流变化；记录的数据再制表分析。,结果针刺实验小鼠A组的所发电流在831A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流的平均差值为35.92A；B组所发电流在41-1A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为21.58A；C组所发电流在1481A区间，一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为68.92A。,表1-2、12只小鼠活时及死后针刺曲池、足三里穴所发电流发生率分析表（A）,No体重gA组A组差值B组B组差值C组C组差值119.428622240241203783218.521417233201024359319.1362349-1101236756421.2369273423243835518.31521316709591346620.08328138335961878718.35885041635381226819.7344303113014840108917.9618531221011211111020.05915824222893861117.3233201101110428761218.52822626521933063合计228.283143141-12591481827均数19.0235.9221.5868.92发生率100100100,分析证明该实验所测电流为针刺电化学效应的依据如下：,AnalysisThecurrentexperimentprovidesfortheevidenceontheeffectsofacupunctureelectrochemical.,A、由于无皮肤的组织电阻为300800，因此实验1-1所测到的-4467A瞬间电流变化区间的电流差值为2773A。而其瞬间电压变化区间（根据欧姆定律）应为：8.1V58.4V，明显远远大于50100mV的细胞动作电位；因此该瞬间电流不是我们已知的生物内在电流。而实验1-2所测的831A、41-1A、1481A瞬间电流变化区间的平均电流差值为35.92A、21.58A、21.58A，并一直呈现由高向低的电流变化，电流发生率为100，应与上述实验1-1的结果相同。,A、Theorganizationskinresistancewas300to800cmonwithoutskin,sothemarginoftransientcurrentchangeswas2773Aontheinterval-4467A.Whiletheinstantaneousvoltage(accordingtoOhmslaw)was8.1V58.4V,obviouslyfargreaterthanthe50100mVofactionpotentials；Assuch,thecurrentwasnotbiologicalinternalcurrentsasweknow。,B、此外，笔者曾在2002年的离体动植物（猪肉、草莓）电化学测定实验中也明显证实了（结果与此次活体实验一样）其间也能产生uA级的电流。张大同.不同材质针灸针的电化学实验，上海针灸杂志，2003，22（5）：33-34.,B、Inaddition，theresultalsowasprovedbyinvitroelectrochemicalplantsandanimalsoftheexperimentin2002(withthesamevivoexperiments)whichcouldalsohaveaClassAAElectricity.张大同.不同材质针灸针的电化学实验.上海针灸杂志,2003.22（5）:33-34.,C、方便揿针电化学科学规律。,C、Theexistingelectrochemicaluniversallawsofscience.,实验1结论针刺穴位组织（能改变电位差）具有电化学原电池效应。,Experiment1ConclusionAcupuncturepoints(tochangethepotentialdifference)havetheoriginalbatteryelectrochemicaleffect.,实验2方便揿针针体的腐蚀研究,Experiment2Clinicalstudiesofacupunctureneedlescorrosion（Thecontrolgroupdidnotjoinbecauseoforiginallimitedtothelength,itwasaddedasfollows）,方法将3600枚方便揿针随机分成实验组（3000枚）和对照组（600枚），在一年中，实验组的3000枚方便揿针在临床反复灭菌使用，对照组600枚方便揿针也随实验组一起反复灭菌但不使用，其间由目测挑选出具有可见腐蚀斑的揿针（数据采用x2检验处理，P0.05有统计学意义），再选典型腐蚀的揿针用电子显微镜放大拍摄分析。,Methods3,600acupunctureneedlesweredividedintotheexperimentalgroup(3000)andthecontrolgroup(600)randomly.3000acupunctureneedleswereusedrepeatedlybysterilization,and600acupunctureneedlesweresterilizedagainandagainastheexperimentalgroupbutwithoutusing，too.Meantime,selectedcorrosionspotwiththeacupunctureneedlebythevisualvisible(dataprocessingusingx2test,P0.05wassignificantly),there-electionoftypicalcorrosionacupunctureneedlestobeamplifiedwithelectronicmicroscope.,结果目测出实验组腐蚀针259枚（电子显微镜：腐蚀针灸针表面呈现部分较严重腐蚀缺损区域），而对照组目测腐蚀针为0枚；实验组与对照组比较，P0.05差异有显著意义。请见具体资料。,Result259needleswerecorrodedintheexperimentalgroup(electronmicroscope:acupunctureneedlesurfacecorrosionshowedsomeofthemoreseriouscorrosiondefectregions),while0needlewascorrodedinthecontrolgroupbyeyeballing;Theresultofexperimentalgroupcomparedwiththecontrolgroup,P0.05significantdifference.Seespecificinformation.,表2、实验组方便揿针针体目测腐蚀率发生分析表Table2、Theexperimentalgroupstainlesssteelacupunctureneedlesvisualanalysisofcorrosionrateintable,时间（月）腐蚀针数未腐蚀针数分析总针数腐蚀百分率Time（Amonth）CorrosionpincountsNocorrosionpincountsThetotalnumberofneedleCorrosionpercentage1303000300004619298130000.637951293029811.711012189274129306.45Total259274130008.63,表3、实验组与对照组目测方便揿针腐蚀率的比较Table3、Theexperimentalgroupandcontrolgroupvisualstainlesssteelacupunctureneedlecorrosionratecomparison,腐蚀针数未腐蚀针数分析总针数腐蚀百分率CorrosionpincountsNocorrosionpincountsThetotalnumberofneedleCorrosionpercentage实验组259（8.63）2741（91.37）30008.63(Theexperimentalgroup)对照组0（0）600（100）6000(Thecontrolgroup)Total259334136007.19x2=55.81562406P0.05,腐蚀揿针的电子显微镜照片所示腐蚀现象见图1、2、3,CorrosionacupunctureneedleshownintheelectronmicroscopephotographsofcorrosionphenomenonSeefigure1,2,3,图1放大50倍的腐蚀方便揿针局部Figure1Large50timesthelocalcorrosionacupunctureneedles,图2放大100倍的腐蚀方便揿针局部Figure2Large100timesthelocalcorrosionacupunctureneedles,图3放大1000倍的腐蚀方便揿针局部Figure3Large1000timesthelocalcorrosionacupunctureneedles,实验2结论方便揿针针刺穴位组织会使针体腐蚀，该腐蚀现象符合电化学腐蚀原理。,Experiment2ConclusionThepointofacupunctureneedleswillcorrodetheacupunctureneedles,corrosionphenomenonisconsistentwiththeprincipleofelectrochemicalcorrosion.,实验3方便揿针针刺穴位组织铬、镍、铁元素含量变化的研究,Experiment3ThestudyonthechangeofelementofacupuncturePointswithchromium,nickel,iron.,方法将15只实验兔按A（揿针10枚刺入左环跳穴区电针仪通电留针5h）、B（揿针10枚刺入右臂臑穴区留针5h）实验组及C（右环跳穴区）、D（右臂臑穴区）对照组，进行实验，5h后对60个（每点15个）样本用(ICP-MS)电感耦合等离子体质谱仪（对与方便揿针主体相关联的铬、镍、铁元素的含量）进行分析，数据采用t检验处理，P0.05有统计学意义。,Methods15rabbitsweredividedintotheexperimentalgroupsandcontrolgroups，theexperimentalgroupswereA(10acupunctureneedlespiercingleftCentralPointdistrictjump-electro-acupunctureneedletelegramtostay5h)，andB(10acupunctureneedlespiercingrightarmHDLPointsdistricttostay5h).ThecontrolgroupswereC(rightringjumpPoint),andD(rightarmHDLPointdistrict.60samples(15perpoint)wereanalyzedbyelectricityflucoupledplasmamassspectrometer(withstainlesssteelacupunctureneedleslinkedtothemainchromium,nickel,ironcontent),dataprocessingusingt-test,P0.05withstatisticalsignificance.,结果实验组铬、镍、铁元素含量大幅升高，与对照组比较，P0.001其差异具显著意义。见表4、表5。,ResultTheexperimentalgroupchromium,nickel,ironcontentsignificantlyincreased,comparedwiththecontrolgroup,P0.001withitssignificantdifference.SeeTable4andTable5.,表4两组臂臑穴区铬、镍、铁元素含量的比较（S，ng/g，各组n=15）Table4、CompareofnumberofCr、Ni、FeinthepointofJianYubetweentwogroups,铬Cr镍Ni铁Fe实验组（留针）270.0168271.63116.1608743.8727060.80411332.91(stayneedle)对照组Control54.00709012.762.73888721.9495523.45362380.729Comparedwiththecontrolgroup（与对照组比较）:P0.001,表5两组环跳穴区铬、镍、铁元素含量的比较（S，ng/g，各组n=15）Table5、ComparedwiththeelementofCr、Ni、FeinthepointofJianliaobetweentwogroups,铬Cr镍Ni铁Fe实验组（电针）303.93884115.7134.7799164.5514175.5915327.276(Electroacupuncture)对照组Control58.01403019.395.31042624.8245414.27642531.767Comparedwiththecontrolgroup（与对照组比较）:P0.001,实验3结论方便揿针针刺穴位组织（能够增加离子、微量元素）具有电化学原电池效应。,Experiment2ConclusionAcupuncturepoints(toincreaseions,traceelements)havetheoriginalelectrochemicalbatteryeffect.,总体结论按上述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，所共同组成的证据锁链。笔者认为：方便揿针针刺穴位组织的原始效应物质为电子、微量元素；针刺穴位组织具有电化学原电池（能够增加电子、离子、微量元素）效应。,Th