白芍总苷对小鼠接触性皮炎的作用及机制研究

A1A0A2A31白芍总苷对小鼠接触性皮炎的作用及机制研究前言接触性皮炎是皮肤、粘膜单次或多次接触外源性物质后，在接触部位甚至以外的部位发生的炎症性反应A4A5A6，是皮肤科常见病、多发病，包括由化学性刺激引起的刺激性接触性皮炎和型变态反应引起的变态反应性接触性皮炎。刺激性接触性皮炎是指接触物对皮肤有很强的刺激性，任何人接触后均可发生皮炎；变态反应性接触性皮炎是指接触物基本上是无刺激性的，少数人在接触该物质致敏后，再接触该物质，经1248H在接触部位及其附近发生皮炎。变应性接触性皮炎是由T细胞介导的抗原特异性皮肤过敏反应，以抗原刺激后局部皮肤出现一系列的皮肤炎症细胞浸润、炎症介质释放为特征。不少文献肯定了型T淋巴细胞包括CD4A7TH1细胞和CD8A7TCL细胞因子在变应性接触性皮炎病变过程中的作用，G1306近G7481G1075有不少文献指出，型G18G266型包括CD4A7TH2细胞和CD8A7TC2细胞T淋巴细胞因子的G2172态G6925变，G5445G2721了变应性接触性皮炎的发G4649和G17728G5414。细胞因子可以G8975化局部G1881皮细胞，G17247化淋巴细胞，上G16855G1039G16213G13464G13467G11468G4493性G3809G2524G1319G48G43C、G2339G2528刺激因子和G21667附G2010子的G15932G17810，在变应性接触性皮炎的发生、发G4649和G17728G5414中起G18337G16213的作用A4A8A9A10A6。在变应性接触性皮炎发病中，G44型细胞因子TG49G41G162起G18337G16213作用。TG49G41G162基因G6982G19512的G4579G21748或G13785应用TG49G41G162中和抗G1319，均不G14033G3827G1147生G7138G7186的变应性接触性皮炎反应A4A11A6。TG49G41G162对G47CG1186G15932皮G2533G3250G8981淋巴G13479的G12239G2172和G3250G8981淋巴G13479G1881G7653G12373G10378细胞的G13870G19610起G1039G16213的作用A4A10A6。TG49G41G162G17836是G18337G16213的炎症G2081介质，可以G16837导炎症反应中多G12193细胞因子的G1147生、G21667附G2010子G3926G44CG36G481和G2339G2528刺激因子的G15932G17810A4A12A9A13A6。G1075是G16837导G49G41G171G37G8975化的G18337G16213细胞因子A4A14A6。G49G41G171G37是一G12879G14033特异性G3332G16794G2047G13479G2524DG49G36的G53G72LG12879G15519G11345质G1120G13870G1319G17728G5417因子A4A15A6。A16A17A18A19A20A21A22A23A21A24A25A262G49G41G171G37可G14033作为G3999发炎症反应的上G9228G10627G14422在G11154病中起G18337G16213作用。G49G41G171G37作为一G12193G1431G17839基因G17728G5417的G7692G17728G5417因子，G2454G994G16855G14422、G6523G2058G1825G11135反应及其G17839程的G1461G2507G1268导G17896G5464；G2520G12193G1319G1881、外因G13044G3926G2520G12193G5506生物及病G8614的G1207G16886G1147物、炎性细胞因子、T细胞及G37细胞的G15932G19766G2010子G12573均可G1363其G8975化A4A27A6。G8975化的G49G41G171G37G2454G994G7438G1319多G12193G15519G11345、G18250及细胞因子的基因G17728G5417A4A5A28A6、G1825G11135G994炎症反应的G16855G14422过程，G3926抗原G17894G2588、TG994G37细胞的激G8975及G12879型G17728G6454以及G1825G11135G5191G15925的G12295定。G49G41G171G37G17902过对G11345细胞介G130441G708G44G471G163G709，G44G472，G44G47G25，G13971G11256G3363G8527因子TG49G41G162、G16837导型一G8699化G8706G2524G18250G76G49G50S、G10627加G8699G182502CG50X2、细胞间G21667附G2010子1G44CG36G481G12573G16837导性G16855G14422作用G2454G994G1825G11135细胞的增殖、G2010化及G1825G11135应答。此外，G1825G11135细胞G1881G49G41G171G37G8975性的G6925变G17836直接G5445G2721G1825G11135细胞的G8975化、增生和凋亡。因此G49G41G171G37的激G8975就可以作为炎性反应的触发器及关键G10627G14422，G16837导G7438G1319一系列炎性反应因子的G1147生A4A27A6，最终导致炎性病理损伤。CD1A是郎格汉斯细胞G708G47ANGG72RHANSCG72LLS，G47CG709的标记性G2010子，而G47C的抗原G17894G2588功G14033在变应性接触性皮炎的发病过程中起G18337G16213的作用。在变应性接触性皮炎发病过程中，每G12193细胞因子的作用都不是单一的，对变应性接触性皮炎的抑G2058或G13785G1431G17839作用G1075不是绝对的。G2520G12193细胞因子之间可以G17902过G2524成G2010泌G11468互G16855G14422、受G1319G15932G17810G11468互G16855G6523、生物学效应G11468互G5445G2721，G1186而G16855G14422变应性接触性皮炎病理过程的G17839G4649和G17728G5414。治疗变应性接触性皮炎的方法是在G16794G2047致病原的G2081提下，去G19512或避G1825再次接触致病原。然而在实际治疗中致病原往往难以发现，或G13785由于变应性接触性皮炎患G13785有时不G14033圆满G6925善他们G994变应原接触的现G10378，G1075不G14033随意G6925变工作G12193G12879，于是应用皮质激G13044治疗成为首选，G1306皮质激G13044的不良反应限G2058了其推广和应用。G11345芍为芍药的干燥根，G11345芍总苷TOTALGLUCOSG76DG72SOFPAG72ONY，TGP为G11345芍中提取的有效成G2010，G1039G16213含有芍药苷、芍药G1881酯苷、羟基芍药苷、苯甲酰A16A17A18A19A20A21A22A23A21A24A25A263芍药苷G12573单萜苷G12879化G2524物。G11345芍总苷的药理及临床研究发现，G11345芍总苷具有止痛、抗炎、保肝，以及多G17896G5464抑G2058自身G1825G11135反应G12573多G12193药理作用，对G12879风湿性关G14422炎、系统性红斑狼疮G12573自身G1825G11135病有确切疗效。现G1207药理研究证实，TGP可抑G2058大G21748腹腔巨噬细胞G2010泌TG49G41G162，G44G471G163，G44G472，G49O，G1147生抗炎作用A4A5A5A6。为了了解G11345芍总苷在变应性接触性皮炎治疗中的作用及可G14033G1147生的作用G7438G2058，本研究G17902过建立G4579G21748变应性接触性皮炎模型，G16278G4531G11345芍总苷对G4579G21748变应性接触性皮炎大G1319G5430态变化和G13464G13467病理学G6925变，G17902过G8991定细胞因TG49G41G162的G8712G5191以及G16278G4531G49G41G171G37、CD1A在G4579G21748G13831G13464G13467中的G15932G17810，G2033G8505G6518G16764G11345芍总G10988治疗变应性接触性皮炎的可G14033性及作用G7438G2058。A16A17A18A19A20A21A22A23A21A24A25A264材料和方法1G7460G70211G171G2172物实G20576用G4579G21748为G6208G5042大学G2319学G19510G2172物中G5527提G1391的G7130G7138G4579G2174842G2494，G19616G19608G2520G2334，G258G2620G21848，G1319G183372G24G22G19G。G12573G13435G726G9177G8917G2070。G4579G21748G20294G1871于G18341G4658G12560中，随意G20290G20147、G20290G8712，G2620G3272G9213G52422G19G263G15湿G5242G25G19G8。1G172G1039G16213G16809G2070和G16809G2070G114302，4G1120G11825基G8691苯G708SG76GG80AG36LDRG76CHG1856G2508G709、G11345芍总苷G708G4437G8886立G2338G2058药G1856G2508G709、G3332G3634G12871G7506G10267G708G9005G8755G1197G10734G2058药G13941G1233有限G1856G2508G709、TG49G41G162G40G47G44SG36G16809G2070G11430G708G8506汉G2350G3775G5515G1856G2508G709、G49G41G171G37、CD1AG1825G11135G13464化G16809G2070G11430G708G8506汉G2350G3775G5515G1856G2508G709。1G17G22实G20576器G7460G994G1214器皮肤圆G5430G6183G4392器G708直G5464G25G80G80G709、G11017子G2010G7524G3837G5191G708上G9035G12946G4506科学G1214器有限G1856G2508G709、数G7186G9228标G2357G4622G708G2283G1152G2283G18339G18339具G7438G11017有限G1856G2508G709、G5506G4392G7507G18250标G1214、G18250标G1214G8939G7507G7438。2实G20576方法2G171G4579G21748变应性接触性皮炎模型建立G7262G171G171DG49G41G37G18209G3803G7262G24G19G80GDG49G41G37加G18494G7261G1319G12227G8616的G1005G18242G994G8216G8024G8845G9163G2524G9094G24G19G80L，G18209成G19G17G24G8的DG49G41G37G9354G9094致敏。G45702G24G19G80GDG49G41G37加G18494G7261G1319G12227G8616的G1005G18242G994G8216G8024G8845G9163G2524G909412G24G80L，G18209成G19G172G8的DG49G41G37G9354G9094G16837发皮炎。2G171G172取G7130G7138G4579G217481G19G2494，G19616G19608G2520G2334，G4579G21748于实G20576G2081一G3837在腹部G3803皮2CG80G1041G17G24CG80，用8G8G11839化G19060G8712G9354G9094G14085G8623，生理G11428G8712G1817G2010G6842G8939。实G20576G12544一G3837、G12544G1120G3837在腹部去G8623部位均G2260G9046G5079G19G17G24G8的2G24G173G47DG49G41G37G9354G9094致敏。实G20576G12544G25G3837在G4579G21748的G5050G13831腹、G13831G13984G19766G9046G5079G19G172G8的2G19G173G47DG49G41G37G9354G9094G16837发皮炎。G2503G13831G9046G50792G19G173G47基质A29A30A31A32。建立G4579G21748变应性接触性皮炎模型。实G20576G12544G26G3837G16278G4531G4579G21748G5050G13831大G1319G5430态变化，G3800G8527后取G5050G13831皮损G15904G13464G13467病理G7828G7609，G2040定G17908模是否成功。2G172G11345芍总苷对G4579G21748变应性接触性皮炎模型的G5445G27212G172G171实G20576G2010G13464G726G17908模成功后，G222G2494G4579G21748被随G7438G2010为4G13464，G2010G2047为空G11345G13464、模A16A17A18A19A20A21A22A23A21A24A25A265型G13464、G3332G3634G12871G7506G13464和G11345芍总苷G13464，每G134648G2494。其中空G11345G13464未做任何G3800理。2G172G172DG49G41G37G16837发模型G726G4579G21748于实G20576G2081一G3837在腹部G3803皮2CG80G1041G17G24CG80，用8G8G11839化G19060G8712G9354G9094G14085G8623，实G20576G12544一G3837、G12544G1120G3837模型G13464、G3332G3634G12871G7506G13464和G11345芍总苷G13464G4579G21748在腹部去G8623部位均G2260G9046G5079G19G17G24G8的2G24G173G47DG49G41G37G9354G9094致敏。实G20576G12544六G3837在G4579G21748的G5050G13831腹、G13831G13984G19766G9046G5079G19G172G8的2G19G173G47DG49G41G37G9354G9094G16837发皮炎。G2503G13831G9046G50792G19G173G47的基质A29A30A31A32。2G17G22给药方法G11345芍总苷G13464G4579G21748G1319G18337用药G18339为G22G19GG5050G2503G4579G2174818G80GG18D药物G2070G18339按每千克G1319质G18339为中G2338人民共和国药典规定临床用G18339的2G19倍，G9354于G19G17G24G8羧甲基纤维G13044G9354G9094中，G4570药物浓G5242校正到实G20576中每G22G19GG4579G21748G1319质G18339灌胃G18339为4G80L，每次取G19G174G80L灌胃。G3332G3634G12871G7506G1319G18337用药G18339为G22G19GG5050G2503G4579G21748G19G17G199G80GG18DG708按成人每G3837G3332G3634G12871G75069G80G，G4579G21748用药G18339为2G19倍G709，G9354于G19G17G24G8羧甲基纤维G13044G9354G9094中，每次取G19G174G80L灌胃。模型G13464每次予以生理G11428G8712G19G174G80L灌胃。实G20576G12544一G3837起G2520G13464自G12544一次G9046药G20812G4579时灌胃，连续用药五G3837，每G3837一次。实G20576G12544六G3837G9046药G20812G4579时及G9046药后G25G4579时灌胃G2520一次。所有G4579G21748于G12544七G3837采用眼球摘G19512法G3800G8527并取血G3803用。G8991G18339G4579G21748双G13831中部厚G5242，用皮肤圆G5430G6183G4392器沿G4579G21748G5050G13831G13831缘中部G6183G4392，所取G5050G13831G13464G13467一G2010为G1120，置于福尔马林中G3803用。一部G2010切G10267G43G40染色，G13464G13467病理G7828G7609，一部G2010G15904G1825G11135G13464化G7828G7609。2G174G16278G4531指标2G174G171G13831厚G5242差G726数G7186G9228标G2357G4622G8991G18339G4579G21748双侧G13831中部的厚G5242G15计算G16837发后G5050G2503G13831厚G5242差。2G174G172G43G40染色及G16278G4531G3800G8527后取G4579G21748G5050G13831G13464G13467常规石蜡切G10267，G43G40染色。用光镜G16278G4531G43G40染色切G10267中有无病理变化和G2520G13464G13467间有无差异，并G16278G4531染色切G10267中细胞G12879型的变化及差异。2G174G17G22G49G41G171G37PG25G24在皮损G3800的G15932G17810G726G4579G21748G5050G13831G13464G13467G17839G15904常规石蜡包埋，连续切G10267。采用G1825G11135G13464化的方法G7828G8991G4579G21748G5050G13831皮损G3800G49G41G171G37PG25G24的G15932G17810G7262G174G17G22G171G9094G1319G18209置G7269G24G8乙醇9G24G80L纯乙醇加G24G80L蒸馏G8712、8G24G8乙醇G7088G24G80L纯乙A16A17A18A19A20A21A22A23A21A24A25A266醇加1G24G80L蒸馏G8712G709、G26G24G8乙醇G708G26G24G80L纯乙醇加2G24G80L蒸馏G8712G709、PG37SG18209方G726G49ACL8G17G19G，KCLG19G172G。12G43A8G50G49AA8PG50A11G22G17G25G22G，KG43A8PG50A11G19G1724G加1G19G19G19G80L蒸馏G8712、G1825G11135G13464化专用PG37SG708PG43G26G172G26G17G25G709G7261G19G19G19G80L蒸馏G8712中加G49ACL8G17G24G，G49AA8G43PG50A112G178G，G49AG43A8PG50A11G19G174GG708G3926果用的G3809G2524G8712的磷酸G11428，应加上G2010子式中G8712的含G18339G709、G19G17G191G48枸橼酸G11428缓冲G9094PG43G25G17G19G5050G2503，1G19G19G19G80L蒸馏G8712，枸橼酸三G49AG708CA13G43A12G49AA10G50A142G43A8G50G709G22G，枸橼酸G708CA13G43A15G50A14G43A8G50G709G19G174G、G7186色G9094G726在G16809管中先加1G80L1G104G43G53P反应缓冲G9094，然后依次加G1849G16809G2070G36G24G19UL，G16809G2070G37G24G19UL，G16809G2070CG24G19ULG9163G2260。2G174G17G22G172G8505骤G726G25G19G263烤箱烘G10267G708G4579G21748G5050G13831G13464G13467石蜡切G10267G709G22G24G80G76N，G14085蜡G1120甲苯G22次，每次1G24G80G76N。梯G5242乙醇G7261G19G19G8乙醇2G104G248G80G76N，9G24G8乙醇2G104G248G80G76N，8G24G8乙醇1G104G248G80G76N，G26G24G8乙醇1G104G248G80G76N，G22G8G43A31G50A31室G92131G191G24G80G76N，蒸馏G8712G22G104G248G80G76N。抗原修G3809G726G4570切G10267浸G1849G19G17G191G48枸橼酸G11428缓冲G9094G708PG43G25G17G19G709，加热G708G5506G8886炉G709至沸腾后盖紧高压锅至气G1319喷出后12G80G76N，切断G11017源，冷却后，G6183开锅盖取出标本。PG37S冲G89392G80G76NG104G22次。G241G19G8G37SG36封闭G9094封闭，每个标本加G1849约1G19G19UL，G22G26G263G104G22G19G80G76N，甩去封闭G9094不G8939，G4570切G10267G2010为两G13464，一G13464均加一抗，一G13464为阴性对照，不加一抗G708一抗1G7261G19G19用PG37S稀释G709。4G263过夜。PG37SG8939G22G1042G80G76N。两G13464切G10267均加G1120抗G708山羊抗G4579G21748G44GG约1G19G19ULG709G15G22G26G2632G19G80G76N，PG37SG8939G22G1042G80G76N，滴加G16809G2070SG36G37CG22G26G2632G19G80G76N，PG37SG89394G104G24G80G76N，DG36G37G7186色，反应1G192G19S，蒸馏G8712G8939涤。G4570切G10267置于苏木G12946染色1G80G76N，取出用4G19G24G19G263G9213G8712G8939涤，9G24G8乙醇G248G80G76N，1G19G19G8乙醇G248G80G76N，G1120甲苯G248G80G76NG14085G8712，透G7138，待G1817G2010干燥，G7653胶封G10267后有棕黄色颗粒G11540染的细胞为G19463性细胞。G16278G4531G49G41G171G37PG25G24的G15932G17810。2G174G174G16278G4531CD1A在G4579G21748G5050G13831皮损G3800G13464G13467的G15932G17810G726方法G2528G49G41G171G37PG25G24。2G174G17G24血G9177TG49G41G162G7828G8991G4579G21748G3800G8527时采用眼球摘G19512法取血约1G17G24G80L，G19757置1G24G80G76N后，G22G19G19G19RG12175G55271G24G80G76N，G2010G12175血G9177，置于2G19G263G1924箱中待G7828，采用G18250G13864G1825G11135G2572附法G708G40G47G44SG36G709G8991定血G9177中细胞因子TG49G41G162G8712G5191。A16A17A18A19A20A21A22A23A21A24A25A2672G174G17G24G171G40G47G44SG36G16809G2070G11430G13464成G18250标G7507G708COATG72DG58G72LLSG7099G25G4392G15G18250标抗G1319工作G9094G708G40NG93YG80G72CONG77UGATG72G70912G80LG151G19G104标本稀释G9094G708SAG80PLG72G37UFFG72RG70912G80LG152G19G104浓G13565G8939涤G9094G708G58ASHG37UFFG72RG709G24G19G80LG15G12544一抗G1319工作G9094G708G37G76OTG76NYLATG72DG36NTG76G69ODYG70912G80L终止G9094G708STOPSOLUTG76ONG70912G80L。2G174G17G24G172G7828G8991G8505骤G726G6922G19610血G9177，2G19G263保G4396。标G1946G2709G9094G18209置G726G1363用G2081加G18491G80L蒸馏G8712G9163G2260，G18209成2G19NGG18G80L的G9354G9094。G16786标G1946管8管，G12544一管加标本稀释G90949G19G19UL，G12544G1120至G12544G1855管加G1849标本稀释G9094G24G19G19UL。在G12544一管中加G18492G19NGG18G80L的标G1946G2709G9354G90941G19G19ULG9163G2260后用加G7691器G2572出G24G19G19UL，G12239至G12544G1120管。G3926此反G3809做对倍稀释，G1186G12544七管中G2572出G24G19G19ULG5335去。G12544G1855管为空G11345对照。1G19G104标本稀释G9094用蒸馏G8712做1G7261G19倍稀释。G8939涤G9094；用G18337蒸G87121G7262G19稀释。加G7691G726每G4392G2520加G1849标G1946G2709或待G8991G7691G27091G19G19UL，G4570反应G7507G1817G2010G9163G2260后置G22G26G26312G19G80G76N。G8939G7507G726用G8939涤G9094G4570反应G7507G1817G2010G8939涤4G25次，G2533G9400G13452上G2372干。每G4392中加G1849G12544一抗G1319工作G90941G19G19UL，G4570反应G7507G1817G2010G9163G2260后置G22G26G263G25G19G2010G19059。G8939G7507G2528G2081。每G4392加G18250标抗G1319工作G90941G19G19UL，G4570反应G7507置G22G26G263G22G19G2010G19059。G8939G7507G2528G2081。每G4392加G1849G5225物工作G90941G19G19UL，置G22G26G263G7275G3800反应1G24G2010G19059。每G4392加G18491G19G19UL终止G9094G9163G2260。G22G19G2010G19059G1881用G18250标G1214在4G24G19NG80出G8991定G2572光G1552。2G174G17G24G17G22计算G13479果G726所有G50DG1552都应G1955G19512空G11345G1552再计算。以标G1946G27092G19G19G19、1G19G19G19、G24G19G19、2G24G19、12G24、G252G17G24、G221G172、G19PGG18G80L为G8190G3364标，G50DG1552为G13449G3364标，在G3364标G13452上G11023G3282，G11023出标G1946G7366G13459。根G6466G7691G2709G50DG1552在该G7366G13459G3282上G7609出G11468应的TG49G41含G18339。2G17G24统计学G3800理统计G17176G7021采用SPSS1G22G17G19统计G17731G1226G17839G15904G2010G7524G3800理。计G18339G17176G7021以均数G102标G1946差G15932G12046，PG728G19G17G19G24有统计学意G1053。A33A34A35A36A37A38A39A40A38A41A42A438结果1小鼠变应性接触性皮炎模型的建立用DG49G41G37G16837发的G4579G21748G5050G13831G13971G13972、G1817血，可见G13831部血管G6205G5364。G13464G13467病理G7186G12046G15932皮细胞间及细胞G1881G8712G13971，G14033发现G17751多的单一G7692细胞及少数多G5430G7692细胞的浸润。G11507皮G13479G13544G13464G13467G8712G13971，血管G2620G3272可见G17743G5242细胞浸润G708见G32821G709。G4579G21748变应性接触性皮炎模型成功建立。G32821G4579G21748变应性接触性皮炎临床G13464G13467病理G6925变G708G43G40G1041G19G19G709G36正常G4579G21748G13831外G16278。G37DG49G41G37G16837发的G4579G21748G13831外G16278G726G13831G13971G13972、G1817血，可见G13831部血管G6205G5364。C正常G4579G21748G13464G13831G13467病理G15932现。DDG49G41G37G16837发的G4579G21748G13831G13464G13467病理G15932现G15932皮细胞间及细胞G1881G8712G13971，G11507皮G13479G13544G13464G13467G8712G13971G15可见单一G7692细胞及少数多G5430G7692细胞的浸润。2各药物对鼠耳厚度的影响G13917眼G16278G4531，模型G13464G4579G21748G5050G13831G13971G13972、G1817血，可见G13831部血管G6205G5364。G3332G3634G12871G7506G13464及G11345芍总苷G13464G4579G21748G5050G13831G13971G13972不G7138G7186，无G7138G7186G1817血。G994模型G13464G11468G8616G3332G3634G12871G7506G13464及G11345芍总苷G13464G16837发后G5050G2503G13831厚G5242差G7138G7186G1955G4579。G3332G3634G12871G7506及G11345芍总苷G14033有效G6925善G13831G13971G13972G5785G1929G708见G159321G709。ABCDA33A34A35A36A37A38A39A40A38A41A42A439G159321G11345芍总苷对变应性接触性皮炎G4579G21748G13831厚G5242差的G5445G2721G708G91G102S，G1041G19A44A45G80G80G709G13464G2047NG2494G13831厚G5242差G708G5050G2503G709空G11345G134648G24G1742G24G102G19G17G24G22G26模型G134648G229G172G19G19G10211G17G2419G11345芍总苷G13464812G17G19G19G19G1021G17G19G19G19G3332G3634G12871G7506G1346481G19G17G22G22G22G1022G17G1982G8892G726空G11345G13464G994模型G13464G11468G8616PG728G19G17G19G24，G11345芍总苷G13464、G3332G3634G12871G7506G13464G994模型G13464G11468G8616PG728G19G17G19G24，有G7186G14891性差异。G11345芍总苷G13464G994G3332G3634G12871G7506G13464G11468G8616，PG730G19G17G19G24，无G7186G14891性差异。3各药物对组织病理学的影响模型G13464G43G40染色切G10267可见G726G15932皮细胞间及细胞G1881G8712G13971，G14033发现G17751多的单一G7692细胞及少数多G5430G7692细胞的浸润。G11507皮G13479G13544G13464G13467G8712G13971，血管G2620G3272可见G17743G5242细胞浸润。G3332G3634G12871G7506G13464及G11345芍总苷G13464G43G40染色切G10267可见G726G15932皮、G11507皮无G7138G7186G8712G13971，炎性细胞浸润G1955少G708见G32822G709。G32822G2520药物对G13464G13467病理的G5445G2721G708G43G40G1041G19G19G709ABCDA33A34A35A36A37A38A39A40A38A41A42A4310G36G726空G11345G13464。G37DG49G41G37模型G13464G15932皮细胞间及细胞G1881G8712G13971，G11507皮G13479G13544G13464G13467G8712G13971，可见单一G7692细胞及少数多G5430G7692细胞的浸润。CG3332G3634G12871G7506G13464G15932皮、G11507皮无G7138G7186G8712G13971，炎性细胞浸润G1955少。DG11345芍总苷G13464G15932皮、G11507皮无G7138G7186G8712G13971，炎性细胞浸润G1955少。4各药物对NFB、CD1A检测结果的影响G726G49G41G171G37PG25G24在变应性接触性皮炎皮损G16294质G5430成细胞G7692G2620和胞G8986G15932G17810。胞G8986及部G2010胞G7692可见棕黄色颗粒G8797G11540。G11345芍总苷G13464及G3332G3634G12871G7506G13464G49G41G171G37PG25G24G15932G17810G7138G7186G1955少甚至G9052G3845，空G11345G13464未见G49G41G171G37PG25G24G15932G17810见G3282G22。G3282G22G2520药物对G13831G13464G13467G49G41G171G37G15932G17810的G5445G2721G708SP染色G1042G19G19G709G36空G11345G13464。G37DG49G41G37模型G13464G16294质G5430成细胞胞G8986及部G2010胞G7692可见棕黄色颗粒G8797G11540。CG3332G3634G12871G7506G13464未见棕黄色颗粒G8797G11540。D；G11345芍总苷G13464G1177少G16780棕黄色颗粒G8797G11540。ABCDA33A34A35A36A37A38A39A40A38A41A42A4311CD1AG726在光镜下细胞膜或胞G8986G2588黄G16100色G7653G7537G10378G12373起的细胞为CD1AG19463性G47C。在模型G13464中细胞可见棕黄色染色颗粒，在G3332G3634G12871G7506G13464、G11345芍总苷G13464及空G11345G13464未见G7138G7186G15932G17810G708见G32824G709。G32824G2520药物对G13831G13464G13467CD1AG15932G17810的G5445G2721G708SP染色G1042G19G19G709G36空G11345G13464。G37DG49G41G37模型G13464可见棕黄色染色颗粒。CG3332G3634G12871G7506G13464未见棕黄色染色颗粒。DG11345芍总苷G13464未见棕黄色染色颗粒。5各药物对血清中细胞因子TNF的影响G726模型G13464G4579G21748血G9177中TG49G41G162G8712G5191G8616空G11345G13464G7138G7186G2331高，经G11345芍总苷及G3332G3634G12871G7506G3800理后的G4579G21748血G9177TG49G41G162G8712G5191G994模型G13464G11468G8616G7138G7186下G19489，G11345芍总苷及G3332G3634G12871G7506G14033G19489G1314变应性接触性皮炎G4579G21748血G9177中TG49G41G162含G18339，抑G2058了变应性接触性皮炎的炎症反应。G708见G159322G709。ABCDA33A34A35A36A37A38A39A40A38A41A42A4312G159322G2520G13464G4579G21748血G9177中TG49G41G162数G1552G8991定G708G91G102S，G104PGG18G80LG709G13464G2047NG708G2494G709TG49G41G162数G1552空G11345G1346481G178G25G24G102G19G17492模型G13464814G172G19G26G102G19G17G2612G11345芍总苷G1346488G17G19G19G19G102G19G1749G26G3332G3634G12871G7506G134648G25G17G22G222G102G19G17848G8892G726空G11345G13464G994模型G13464G11468G8616PG728G19G17G19G24，G11345芍总苷G13464、G3332G3634G12871G7506G13464G994模型G13464G11468G8616PG728G19G17G19G24，有G7186G14891性差异。实G20576G2081G2520G13464G4579G21748G1319G18337无G7138G7186差异，G2520G13464给药过程中G1319G18337G11065有下G19489，G1306差异无统计学意G1053，G16840G7138G11345芍总苷对G4579G21748生理及G1207G16886功G14033无G7138G7186G5445G2721，G2520G13464G2172物一G14336G10378G1929良G3921。A33A34A35A36A37A38A39A40A38A41A42A4313讨论变应性接触性皮炎G708G36LLG72RGG76CCONTACTDG72RG80ATG76TG76SG15G36CDG709是皮肤或粘膜接触致敏物后，由T细胞介导的G17843发性变态反应引起的炎症性皮肤病。在皮肤接触抗原以后，G7403格汉斯细胞G47CG15932G19766G48G43CG266G12879G2010子的G15932G17810增加，并G1000开G3999G1186G15932皮G2533局部淋巴G13479G12239G2172。在局部淋巴G13479，G47CG4570抗原提G2588给原G3999T细胞A46A47A48A49A45A50G15G17837G7691抗原就G17902过刺激G8975化G47CG4448成了G16837导致敏的过程，G13971G11256G3363G8527因子TG49G41G162G994G11345介G13044G44G471G163对G47C的G8975化、G12239G2172和抗原的提G2588起G18337G16213作用A46A45A50。G7403格汉斯细胞的抗原提G2588功G14033在变应性接触性皮炎的发病过程中起到G18337G16213的作用，CD1A是G7403格汉斯细胞的标记性抗G1319。G16294质G5430成细胞G708KCG709是G2454G994皮肤G1825G11135反应的G18337G16213细胞之一。G2520G12193刺激可G1363KCG2524成或G1147生多G12193生物G8975性G2010子G3926细胞因子及抗G5506生物