如何用NCBI和uniprot数据库查找目的蛋白的氨基酸序列或目的基因的碱基序列mRNA序列

1、运用NCBI数据库査找目得蛋白序列或目得碱基序列第1种方法1、打开NCBI官网,并选择Gene菜单，如下图、% NC8I Resources 9 How To2NCBI Naoort3i Center (ofBFw Joj 匸小 心，/ AssemblyBoProiect Bosampie BoSystcTO Books Cinvar CloneCOfYserxGd Donans dtX3aP dbVar ESTGeneAll DatabasesSearchHCBI Homoto NCBIRoccMircQ List (A Z)All ResourcesChemicals & BoasssDa

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3、ta to you* computerFind hdp documents attend a class or watch a tutondPopular ReiourcPubMedBookah出PubMod CentralPbbUR H=-aHhElAST tJucteoiideGenomeSTIPGeneRoteinPubChcnProteinsSequence AnalyseTaxctiofnyTramog & TutoiialsVarsiionDevelopUs* NCBI APIs and code litxars to Duid 3 跑 icamngAnalyzeIdEify tJ

4、CBI tool fx your data analysis la皱ResearchExmore NCB learch arrf coliacooi pro；easNCBI AnnouncerdOVar JUy2016 Ca new 1000 G 的 ows arorrtsTXa dK5/or lUo 7fl 1；31st NCB k2 在搜索栏内输入想要查找得蛋白名称或基因名称，此处以基因RSK2为例，如图。Gano sources GenomeCaioaonasAnnctatedficnesPr*nworgSDqiKfco corcrxCCDSEraemslRetSeqRetScqGene

5、S3耐 CurrentChrTosome UxatSoM.more.CZ刖iCMTaeolar 20 曲Son Rele*ance -Send to -FiKea;F他场Pm Laai9 对汕 1 thscanlinuwl a rp4:哎d Hen*.S*reb deuiHDid you mean RSK2 as a gene srrtxa* Soatch Gone for RSK2 as a 4*fmCclHacreGene IODescnpticnLocztian/JasesMIMKS?AllQE BFS6KA3nbesonsi ;o(em S6 Mg) A3咖 X.CIS. HU-3.

6、ISFK-130W5ID 6107Wcn0 “HP ihunsilNO 000023 11htKPWKlB. MRX19. RSK.0149911 2026714RSK2. SGK.alO e)M2corhmertPtWRSK2E加跑3nboso曲 croton S6 畅0?Chrawoe X.MAPKAPK-10. MPK-9 Re*2I SearchIO 110651FoPfpyide 3euosW20 0000&S 7S&* 10)W 0C43M 4DmetOGV596 RSK. R?k.22S2S9U 22536371)SGOI dRSK igr rsk rk2Q RSK2 AMD (

7、3IWC匚rz6心Lnbosomii protein S6 kinaMS(MI M0 rps6*a3 Be rsk2ID 399376SOkDd poVpiHfe 3 L homeciou5 Homo sapiens rib3、在搜索结果中查找目得蛋白，注意Descripti o n栏会标明物种信息，以human为例.Aliase s 栏会列出目得蛋白得各种别名。Search resultsItems: 1 to 20 or 203O Sqp 合Iso 1 disuonlinugd of lEQlacfdPage 1 j of 11 NextLastNan-e/Gene IDDescripti

8、onLocationAliasesMIMF RPS6KA3ID 6197ribosorral protein S6 kinase A3 Homo sap心s (hurran)Chromosome X,NC 000023.11 (20149911.20267614, complement)CLS、HU3： ISPK-1, MAPKAPK1B MRX19. RSK RSK2. S6K-alpha3. p90-RSK2. po90RSK23000764在S e a rc h resul t页而中点击您要找得蛋白需称(Name / Gene ID),进入页而并在页而 找到“ ” “ NCBI R e

9、fer e nc e S e quences (R e fScq)”条目，并在其子条目中找到m RN A and Pr o t e in(s)”，查找mRNA碱基序列点击NM,查找蛋白氨基酸序列点击N P,以下以査找mRNA碱基序 列为例如下： NCBI Reference Sequences (RefSeq)日 RefSeqs maintained independently of Annotated Genomes-These reference sequences exist independently of genome builds. ExplainGenomic1. N G_007

10、48&*1 RefSeqGeneRange 5001.12 仃 22Dov/nload GenBank. FASTA. Seouenee Viewer (Gahics：mRNA and Protein(s1. NM 004586.2 NP 004577.1 ribosomal protein S6 kinase alpha-3See identical proteins and their annotated locations for NP 004577.1点击NM004 58 6 . 2进入页面后如下:LOCUS DEFB4ITI0H ACCESSION VERSION KEWRDSS0U

11、FCECiF.GAfTISHHomo sapiens ribosomal protein S6 kinase A3 (RPS6KA3), mRNANCBI Reference Sequence: NM_004586.2FASTA GimphicsGo to NM_0045867723 bp mKUA linear PRI 03-KAE-2016Kcmo sapiens ribosonal protein S6 kinase A3 (RPS6KA3), mKJLL ML004586 刃匸939339 XM_944U2KJLOO4586.2 GI: 56243494RcfScq Homo sapi

12、ens (hunan)Homo sauaemEukzLryo-ta. Metazoa. Chordata. Cianiata, Vertcb匚“ta. Eutclcostomi; Kanunalia. Eutheria. Euarchonloglires; Priniates; Hap lor r bin i. Catatrhirii; Hoiriinidae : Homo.在页而下方会列出mRNA得序列:ORIGIN161121caaactgaag aagtcagtat caaagaaatt gcaatcacac atcatgt aaa ggaaggacat181gaaaaggcag atc

13、cttccca gtttgaactt ttaaaagtat tagggcaggg atcatttgga241aaggttttct tagttaaaaa aatctcaggc tctgatgcta ggcagcttta tgccatgaag301gtattgaaga aggccacact gaaagttcga gaccgagttc ggacaaaaat ggaacgtgat361atcttggtag aggttaatca tccttttatt gtcaagttgc attatgcttt tcaaactgaa421gggaagttgt atcttatttt ggattttctc aggggagga

14、g atttgtttac acgcttatcc481aaagaggtga tgttcacaga agaagatgtc aaattctact tggctgaact tgcacttgct541tt agaccatc tacatagcct gggaataatt t at agagact taaaaccaga aaat at actt601cttgatgaag aaggtcacat caagttaaca gatttcggcc taagtaaaga gtctattgac661catgaaaaga aggcatattc tttttgtgga actatatggctcc agaagtagtt721781at

15、 gettactg gtacactccc tttccaagga aaagatcgaa aagaaacaat gactatgatt841cttaaagcca aacttggaat gccacagttt ttgagtcctg aagcgcagag tcttttaega901atgcttttca agcgaaatcc tgcaaacaga ttaggtgcag gaccagatgg agttgaagaa961attaaaagac attcattttt ctcaacgata gaetggaata aactgtatag aagagaaatt1021catccgccat ttaaacctgc aacggg

16、cagg cctgaagata cattctattt tgatcctgag1081tttactgcaa aaactcccaa agattcacct ggcattccac ctagtgetaa tgcacatcag1141ctttttcggg ggtttagttt tgttgctatt acctcagatg atgaaagcca agetatgeag1201acagttggtg tacattcaat tgttcagcag ttacacagga acagtattca gtttactgat1261ggatatgaag taaaagaaga tattggagtt ggctcctact ctgtttgc

17、aa gagatgtata1321cataaageta caaacatgga gtttgcagtg aagattattg ataaaagcaa gagagaccca第2中方法io进入Uni plot数据库，在搜索栏中直接输入要查找得基因或蛋白名称，如下图:The mis引on of UniProt is to provide the scientific community with a comprehensive, high-quolity and freely acces引ble resource of piUniProtKBUniProt KnowledgebaseSwiss-Prot

18、(551,705):刃 Manually annotatedand reviewedTrEMBL (65.378.749)Automatically annotated and not reviev/ed.CiaHinc ortoH2O进入以下页而:UniRefSequence clustersUniParcSequence archiveProteomesSupporting dataUterature citationsTaxonomySubcellular locationss中0Cross-ref. databasesDiseasesKeywords艺力 入gXYXI IniPrnf

19、Hata.Uni Proc心2X心湖皿 Q Scare一 一 .“ BLAST Aln fU(rtaz/ID 吟)Mg一 亠一一 ”幅花詞UniProtKB results About Pro3 QQQ1 to of 30 A sfton EFilter byk Seeded (0)TrtBLPopular organisms anan 團 C*VyEMry nameJVoCeiw Ccoe *mcsOronismD 051912K56A?_MAW*KtMXontalWUiuim dlpt)d-3KUPKAPK1B, RK2hcw wpwt (Huwan)MOD P1W54K50A3.M3US

20、EMQ5omal protein S olptto-3RpsOka? HjpMpklb.砂2Mr musoAis :隔jn)MO阴x w0o*ne (3) 际O)El Q1S349K50A2_MJMaMMononiAl prctc狼 S Himm? olptio-2RPWCA? rAPKfiPKJC. RSK3Memo(Murvn)733O 8UT3KS6A2_MDUSeKbosonial fvotaln S6 hifwiMi alpha 2NUpkapklc.32M-n rratoAxs :Moufc?33XBA2r。莎82KKOUMLMAKMitoatt-activdtMl procoaf

21、iMfM 1MAPK1 ERK2, PRKMl. PRKM2(Hunan)3603tKr orgjincnaD P27301MltoQci*- actwoted protm3MAPK3 ERK1, PKM3Homo wpws (Huwanj379E 3095K*01_MOUSMitoQcn-flcttvotcd procn MnaM! IMopkl E*2. M0 PrtjnlMr rrwsoAn :358Did you nMdi* 依2? 3,373 fatUt：i) fco-dA?1 Reuewl K-a丄7710/gene=,YRPS6KA3/gene_synon7TTi=rrCLS; HU-3; ISPK-1; JitAPKAPKIB; MRS 19;P0O-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3ORIGIN1611211812413013614214815416016617217818419019611021108111411201126113