丹参对非酒精性脂肪肝大鼠血清MDASODTNFLeptin的影响精

1、丹参对非酒精性脂肪肝大鼠血清 MDA SOD TNFLeptin的影响【摘要】目的观察丹参对非酒精性脂肪肝大鼠血中丙二醛（MDA、过氧化物歧化酶（SOD、肿瘤坏死因子a （TNF a ）、瘦素（Leptin ）的调节作用。方法59只大鼠随机分为5组，第1组为正常饮食 对照组（正常组），11只，喂饲普通饲料；第2-5组为实验组，喂饲高脂饲 料。其中第2组为高脂饮食组（模型组），16只；第3、4组为丹参注射液治 疗大、小剂量治疗组（治疗组），各 11只；第5组为为还原型谷胱甘肽对照组（对照组），10只。治疗组大鼠每天丹参注射液 3.3ml/Kg、1.65ml/Kg ;对照 组每天还原型谷胱甘肽溶液

2、10ml/Kg ;正常组和模型组腹腔注射等量生理盐 水，连续8W观察肝组织的病理改变，比色法测定血清 MDA SOD放免法测定 血清中TNF a、Leptin含量。结果模型组大鼠肝脏脂肪沉着面积，沉着 程度及纤维化程度积分均较正常组明显增多，血清中MDA TNF a含量均较正常组明显升高（P0.01, P0.05）,而SOD Leptin含量较正常组明显减低（PvO.05）。丹参大剂量治疗组大鼠肝脏脂肪沉着面积积分较模型组明显减少（P0.05），血清中MDA含量较模型组明显降低（P0.05）, Leptin含量较模 型组明显升高（P0.05）。结论大剂量丹参可改善非酒精性脂肪肝大鼠肝脏脂肪变性

3、情 况，并能减少脂肪肝大鼠血中 MDA含量，提高Leptin的表达。【关键词】丹参；脂肪肝；大鼠；MDA SOD TNF LeptinAbstract: Objective To observe the effect of Dan she n on thenon alcoholic fatty liver rats MDA, SOD, TNF, Leptin in blood serum.Methods Fifty nine rats were ran domly divided into five groups,the normal group had eleven, the model g

4、roup had sixteen，the largedose Dan she n treatme nt group had eleve n, the low dose Dan she n treatme nt group had eleve n, and the con trol group had ten. The no rmal group rats were fed with com mon feedstuff. The rest groups rats were fed on the high fat diet .To last eight weeks,all rats were pu

5、t to death, tested the contents of MDA, SOD, TNF, Leptin in blood serum and observed pathologic change in rats liver. Result The model group rats liver happened fat pigmentation obviously, and could be found the liver fibrous dege nerati on. The contents of MDA, TNFain fatty liver rats in blood seru

6、m in creased ( P0.01, P0.05), andthe contents of SOD,Leptin degraded ( P0.05) . The fat pigmentation of the large dose Dan she n treatme nt group rats liver was decreased(P0.05) . The contents of MDA in blood serum of the large dose Dan she n treatme nt group were decreased obviously( to compare wit

7、h the model group: P0.05),a nd the contents of Lepti n were in creased obviously( to compare with the model group: P leptin试剂盒购自北方生物技术研究所。1.3 主要仪器电子天平FA2004 （上海精天天平厂），玻璃搅拌器（上海医用仪器厂）， 722紫外分光光度计（上海分析仪器厂制造），光学显微镜为日本 Olympus株 式会社产品，OlympusAU64（生化分析仪为日本株式会社产品。计数仪（西安二六二厂），冷冻离心机。2 方法2.1分组与模型制备大鼠正常喂养1周后随机分为5组，第1组为正常饮食对照组（正常 组），11只，服普通饲料；第25组为脂肪肝组（服高脂饲料，即基础饲料 77.5%，猪油8%胆固