IdentificationofProteinRemainsinArchaeologicalPotsherdsProteomics

1、identification of protein remains in archaeological potsherds by proteomicscaroline solazzo, william w. fitzhugh, christian rolando, and caroline tokarsklreporter: rao huiyuncontent introduction experiment results and discussion application conclusion thinking about the paperintroductionmethods of p

2、rotein analysisstaining methodsantibody specificitygc and lc ? 13c and ? l5n stable isotope ratio measurements (isotope ratio mass spectrometry)peptide mass fingerprinting and peptide sequencing methodsproteomic technologies adapted to the small sample amountprotein extractioncontrolled hydrolysison

3、line nanochromatography- tandem mass spectrometrybioinformatics in this papermethodology development, evaluated on different residues from heated cetacean and pinniped muscle tissues and blubberspresents the results obtained on the archeological sample using the developed methodologyexperimentmethod

4、sprotein extraction (1% tfa)enzymatic hydrolysis (trypsin)sample analysis and database searchpeptide mass fingerprinting spectra maldi-tof-ms(基质辅助激光解吸电离飞行时间质谱基质辅助激光解吸电离飞行时间质谱)the peptide sequence spectra nano lc, nano esi-qq-ft-icr ms/ms（纳流量液相色（纳流量液相色谱，电喷雾傅立叶变换离子回旋共振串联质谱）谱，电喷雾傅立叶变换离子回旋共振串联质谱）the swi

5、ss prot database基质辅助激光解吸电离飞行时间质谱基质辅助激光解吸电离飞行时间质谱傅立叶变换离子回旋共振质谱傅立叶变换离子回旋共振质谱m/zfresult and discussionmaldi-tof-ms was used for the verification of protein extracting solution efficiency. nano lc, nano esi-qq-ft-icr ms/ms was used for peptide sequencing. result for maldi-tof-msresult for maldi-tof-msdi

6、scussion no other protein could be identified despite the presence of numerous peptides on the spectrum; this is probably the consequence of the poor harbor seal protein information in the databases (22 proteins in the swissprot database and 66 in the trembl database）. similar results were obtained

7、from the other analyzed cetacean and pinniped samples.result for nano lc, nano esi-qq-ft-icr ms/ms result for nano lc, nano esi-qq-ft-icr ms/ms result for nano lc, nano esi-qq-ft-icr ms/ms discussionthese results highlight that the proposed extraction and analysis methodologies succeed in extracting

8、 muscle tissue and blubber proteins of cetaceans and pinnipeds and allowed the identification of specific markers of seal species or cetacean and pinniped species. applicationsample (potsherd fragment)1200 - 1400 ad the lnupiat archeological site of point barrow in north alaska. 3cm width methodspro

9、tein extraction (1% tfa)enzymatic hydrolysis (trypsin)sample analysis and database searchthe peptide sequence spectra nano lc, nano esi-qq-ft-icr ms/ms（纳流量液相色（纳流量液相色谱，电喷雾傅立叶变换离子回旋共振串联质谱）谱，电喷雾傅立叶变换离子回旋共振串联质谱）the swiss prot databaseresult resultresultdiscussionas a result , it can be emphasized that t

10、hese two peptides give us significant information concerning the presence of marine species in the archeological potsherd and more precisely the presence of seal species. conclusionthis work clearly confirm the potential and support of ft-icr ms/ms in the case of difficult proteomic analysis such as art and archeological sample studies. these results underline and confirm archeological and ethnological studies proving the importance of seal hunting for inupiat eski