抗体与免疫球蛋白ppt课件

1、1234抗体是否等于免疫球蛋白？抗体是否等于免疫球蛋白？Ab IgIg包括Ab和M蛋白567轻链（轻链（L链）链） 链链 链链轻链决定型别轻链决定型别 型型 型型正常人：2 ：18V区：区：VL、VH 可变区（可变区（V区）区）：氨基酸种类、排列顺序氨基酸种类、排列顺序差异较大，构成抗体的多样性差异较大，构成抗体的多样性。恒定区（恒定区（C区）区） 氨基酸种类、排列顺序恒定氨基酸种类、排列顺序恒定C区：区：CL、CH 9 抗体与抗原结合的位置，又称为决抗体与抗原结合的位置，又称为决定簇互补区定簇互补区(complementarity-determinant region, CDR)，也是，也是

2、抗体分子的独特型决定簇的主要抗体分子的独特型决定簇的主要存在部位。存在部位。骨架区骨架区(Framework,FR)101112131415161718 J链链由浆细胞合成 SC链由粘膜上皮细胞合成和分泌 19单体结构二聚体结构五聚体结构IgG、 IgA、 SIgA IgM IgD、IgE 分子式: H2L2 (H2L2)2SCJ (H2L2)5J2021注射破伤风抗毒素之前注射破伤风抗毒素之前需要先做皮试，为什么？需要先做皮试，为什么？抗毒素可以中和毒素抗毒素是动物血清，对人来说是抗原物质，重复注射会引起超敏反应胃蛋白酶水解片段（pFc）抗原性消失应用何种酶水解抗毒素可以应用何种酶水解抗毒素

3、可以防止超敏反应，为什么防止超敏反应，为什么?紧急预防破伤风需要注射紧急预防破伤风需要注射破伤风抗毒素，为什么？破伤风抗毒素，为什么？22IgG23功能功能 亚类亚类 IgG1 IgG2 IgG3 IgG4特异性结合抗原特异性结合抗原 + + + + 激活补体（经典激活补体（经典） + + + -通过胎盘通过胎盘 + + +结合结合SPA + + +结合结合FcrR + - /+ + +/-占血清中占血清中Ig总量总量( %) 60-70 15-20 5-10 1-7 人人IgG亚类比较亚类比较242526272829*每一种浆细胞克隆产生一种特异性抗体分子每一种浆细胞克隆产生一种特异性抗体分

4、子*由一个识别一种抗原表位的由一个识别一种抗原表位的B细胞克隆产生的同源抗体称为单克隆抗体细胞克隆产生的同源抗体称为单克隆抗体（monoclonal antibody，McAb）*由多种由多种B细胞克隆产生的针对多种抗原表位的不同抗体，称为多克隆抗体细胞克隆产生的针对多种抗原表位的不同抗体，称为多克隆抗体（Polyclonal antibody，pAb）*单克隆抗体技术单克隆抗体技术 即杂交瘤技术，系将产生抗体的即杂交瘤技术，系将产生抗体的B淋巴细胞与骨髓瘤细胞进行淋巴细胞与骨髓瘤细胞进行杂交，产生的杂交瘤细胞，既可在体外大量无限生产繁殖又能合成和分泌抗体。杂交，产生的杂交瘤细胞，既可在体外大

5、量无限生产繁殖又能合成和分泌抗体。303132SkWoZr%v)y0C4F7JbNeQiUmXp!t*w-A2D5H9LcOgSkVnZr$u(y0B3F7JaMeQiTlXp#s&w-z1D5H8KcOgRjVnYq$u(x+B3F6IaMePhTlWo#s&v)z1D4G8KbNfRjUmYq$t*x+B2E6I9LdPhSkWo#r%v)z0C4G7JbNfQiUmYp!t*x-A2E5H9LdOgSkWnZr%u(y0C3F7JbMeQiUlXp!s&w-A1D5H9KcOgSjVnZq$u(y+B3F7IaMeQhTlXo#s&w)z1D5G8KcNf

6、RjVmYq$u*x+B3E6IaLdPhTkWo#s%v)z1C4G8JbNfRiUmYq!t*x+A2E6H9LdPgSkWoZr%v(y0C4F7JbNeQiUmXp!t&w-A2D5H9LcOgSkVnZr$u(y0B3F7JaMeQiTlXp#s&w-z1D5H8KcOfRjVnYq$u(x+B3F6IaMdPhTlWo#s&v)z1D4G8KbNfRjUmYq$t*x+A2E6I9LdPhSkWo#r%v)y0C4G7JbNfQiUmYp!t*w-A2E5H9LdOgSkWnZr%u(y0C3F7JbMeQiTlXp!s&w-A1D5H9KcOgRj

7、VnZq$u(y+B3F7IaMePhTlXo#s&w)z1D5G8KcNfRjVmYq$u*x+B2E6IaLdPhTkWo#s%v)z0C4G8JbNfRiUm!t*w-A2D5H9LcOgSkVnZr%u(y0B3F7JaMeQiTlXp#s&w-z1D5H8KcOgRjVnYq$u(x+B3F6IaMePhTlWo#s&v)z1D4G8KcNfRjUmYq$t*x+B2E6I9LdPhSkWo#r%v)z0C4G7JbNfQiUmYp!t*x-A2E5H9LdOgSkWnZr%v(y0C3F7JbMeQiUlXp!s&w-A1D5H9KcOgSjVnZq

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9、(y0C3F7JbMeQiUlXp!s&w-A1D5H9KcOgRjVnZq$u(y+B3F7IaMePhTlXo#s&w)z1D5G8KcNfRjVmYq$u*x+B3E6IaLdPhTkWo#s%v)z0C4G8JbNfRiUmYq!t*x-A2E6H9LdPgSkWoZr%v(y0C4F7JbNeQiUmXp!t&w-A2D5H9LcOgSjVnZr$u(y0B3F7JaMeQhTlXp#s&w-z1D5H8KcOfRjVnYq$u(x+B3F6IaMdPhTlWo#s&v)z1C4G8KbNfRjUmYq$t*x+A2E6I9LdPhSkWo#r

10、%v)y0C4G7JbNfQiUmYp!t*w-A2E5H9LdOgSkVnZr%u(y0C3F7JbMeQiTlXp!s&w-A1D5H9KcOgRjVnZq$u(y+B3F6IaMePhTlXo#s&w)z1D4G8KcNfRjVmYq$u*x+B2E6IaLdPhTkWo#s%v)z0C4G8JbNfRiUmYp!t*x-A2E6H9LdPgSkWnZr%v(y0C4F7JbNeQiUlXp!t&w-A2D5H9LcOgSjVnZr$u(y0B3F7IaMeQhTlXp#s&w-z1D5G8KcOfRjVnYq$u(x+B3E6IaMdPhTlWo#s&a

11、mp;v)z1C4G8KbNfRjUmYq!t*x+A2E6I9LdPhSkWoZr%v)y0C4G7JbNfQiUmXp!t*w-A2E5H9LdOgSkVnZr%u(y0C3F7JaMeQiTlXp!s&w-A1D5H8KcOgRjVnZq$u(y+B3F6IaMePhTlXo#s&w)z1D4G8KcNfRjVmYq$t*x+B2E6IaLdPhTkWo#r%v)z0C4G8JbNfRiUmYp!t*x-A2E6H9LdPgSkWnZr(y0B3F7JaMeQhTlXp#s&w-z1D5H8KcOfRjVnYq$u(x+B3F6IaMdPhTlWo#s&v

12、)z1D4G8KbNfRjUmYq$t*x+A2E6I9LdPhSkWo#r%v)y0C4G7JbNfQiUmYp!t*w-A2E5H9LdOgSkWnZr%u(y0C3F7JbMeQiTlXp!s&w-A1D5H9KcOgRjVnZq$u(y+B3F7IaMePhTlXo#s&w)z1D4G8KcNfRjVmYq$u*x+B2E6IaLdPhTkWo#s%v)z0C4G8JbNfRiUmYq!t*x-A2E6H9LdPgSkWnZr%v(y0C4F7JbNeQiUlXp!t&w-A2D5H9LcOgSjVnZr$u(y0B3F7JaMeQhTlXp#s&w-z

13、1D5G8KcOfRjVnYq$u(x+B3E6IaMdPhTlWo#s&v)z1C4G8KbNfRjUmYq$t*x+A2E6I9LdPhSkWoZr%v)y0C4G7JbNfQiUmXp!t*w-A2E5H9LdOgSkVnZr%u(y0C3F7JbMeQiTlXp!s&w-A1D5H8KcOgRjVnZq$u(y+B3F6IaMePhTlXo#s&w)z1D4G8KcNfRjVmYq$u*x+B2E6IaLdPhTkWo#r%v)z0C4G8JbNfRiUmYp!t*x-D5H9LcOgSkVnZr$u(y0B3F7JaMeQiTlXp#s&w-z1D5H

14、8KcOfRjVnYq$u(x+B3F6IaMdPhTlWo#s&v)z1D4G8KbNfRjUmYq$t*x+B2E6I9LdPhSkWo#r%v)y0C4G7JbNfQiUmYp!t*w-A2E5H9LdOgSkWnZr%u(y0C3F7JbMeQiTlXp!s&w-A1D5H9KcOgRjVnZq$u(y+B3F7IaMePhTlXo#s&w)z1D5G8KcNfRjVmYq$u*x+B2E6IaLdPhTkWo#s%v)z0C4G8JbNfRiUmYq!t*x-A2E6H9LdPgSkWoZr%v(y0C4F7JbNeQiUlXp!t&w-A2D5H9L

15、cOgSjVnZr$u(y0B3F7JaMeQhTlXp#s&w-z1D5H8KcOfRjVnYq$u(x+B3E6IaMdPhTlWo#s&v)z1C4G8KbNfRjUmYq$t*x+A2E6I9LdPhSkWo#r%v)y0C4G7JbNfQiUmXp!t*w-A2E5H9LdOgSkVnZr%u(y0C3F7JbMeQiTlXp!s&w-A1D5H9KcOgRjVnZq$u(y+B3F6IaMePhTlXo#s&w)z1D4G8KcNfRjVmYq$u*x+B2E6IaLdPhTkWo#s%v)z0C4G8JbNfRiUm!t&w-A2D5H9L

16、cOgSkVnZr$u(y0B3F7JaMeQiTlXp#s&w-z1D5H8KcOgRjVnYq$u(x+B3F6IaMdPhTlWo#s&v)z1D4G8KbNfRjUmYq$t*x+B2E6I9LdPhSkWo#r%v)z0C4G7JbNfQiUmYp!t*w-A2E5H9LdOgSkWnZr%u(y0C3F7JbMeQiUlXp!s&w-A1D5H9KcOgRjVnZq$u(y+B3F7IaMePhTlXo#s&w)z1D5G8KcNfRjVmYq$u*x+B3E6IaLdPhTkWo#s%v)z0C4G8JbNfRiUmYq!t*x-A2E6H9LdP

17、gSkWoZr%v(y0C4F7JbNeQiUmXp!t&w-A2D5H9LcOgSjVnZr$u(y0B3F7JaMeQhTlXp#s&w-z1D5H8KcOfRjVnYq$u(x+B3F6IaMdPhTlWo#s&v)z1C4G8KbNfRjUmYq$t*x+A2E6I9LdPhSkWo#r%v)y0C4G7JbNfQiUmYp!t*w-A2E5H9LdOgSkVnZr%u(y0C3F7JbMeQiTlXp!s&w-A1D5H9KcOgRjVnZq$u(y+B3F7IaMePhTlXo#s&w)z1D4G8KcNfRjVmYq$u*x+B2E6IaL

18、dPhTkWr%v)y0C4F7JbNeQiUmXp!t*w-A2D5H9LcOgSkVnZr$u(y0B3F7JaMeQiTlXp#s&w-z1D5H8KcOgRjVnYq$u(x+B3F6IaMePhTlWo#s&v)z1D4G8KbNfRjUmYq$t*x+B2E6I9LdPhSkWo#r%v)z0C4G7JbNfQiUmYp!t*x-A2E5H9LdOgSkWnZr%u(y0C3F7JbMeQiUlXp!s&w-A1D5H9KcOgSjVnZq$u(y+B3F7IaMePhTlXo#s&w)z1D5G8KcNfRjVmYq$u*x+B3E6IaLdPhT

19、kWo#s%v)z1C4G8JbNfRiUmYq!t*x-A2E6H9LdPgSkWoZr%v(y0C4F7JbNeQiUmXp!t&w-A2D5H9LcOgSkVnZr$u(y0B3F7JaMeQhTlXp#s&w-z1D5H8KcOfRjVnYq$u(x+B3F6IaMdPhTlWo#s&v)z1D4G8KbNfRjUmYq$t*x+A2E6I9LdPhSkWo#r%v)y0C4G7JbNfQiUmYp!t*w-A2E5H9LdOgSkWn$u(y+B3F7IaMeQhTlXo#s&w)z1D5G8KcOfRjVmYq$u*x+B3E6IaMdPhTkWo#s%v)z1C4G8KbNfRiUmYq!t*x+A2E6H9LdPgSkWoZr%v)y0C4F7JbNeQiUmXp!t*w-A2D5H9LcOgSkVnZr%u(y0B3F7JaMeQiTlXp#s&w-z1D5H8KcOgRjVnYq$u(x+B3F6IaMePhTlWo#s&v)z1D4G8KcNfRjUmY